Mature Adipocyte-Derived Dedifferentiated Fat Cells Can Transdifferentiate Into Neural Cells And Promote Functional Recovery From Spinal Cord Injury-Induced Motor Dysfunction In Rodents

2009 Fiscal Year Final Research Report

• Project/Area Number: 19580348
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Nihon University
• Principal Investigator: KANO Koichiro Nihon University, 生物資源科学部, 准教授 (80271039)
• Research Collaborator: NOBUSUE Hiroyuki 日本大学, 生物資源科学部, 博士研究員 ; OKI Yoshunao 日本大学, 生物資源科学部, 助手 (70525667)
• Project Period (FY): 2007 – 2009
• Keywords: 脂肪細胞 / 脱分化 / 分化転換 / 神経系細胞

Research Abstract

When mature adipocytes were isolated from adipose tissue and grown in ceiling culture, transformation into fibroblast-like cells without lipid droplets occurred. These fibroblast-like cells, termed de-differentiated fat (DFAT) cells, could proliferate and could also redifferentiate into mature adipocytes. DFAT cells expressed neural markers such as nestin, NSE, βIII tubulin, NF, S100, GFAP and CNPase. Allografting of DFAT cells into spinal cord injury (SCI)-induced rodents led to significant recovery from hindlimb dysfunction. Grafted cells were detected at the injection site, and some of these cells expressed βIII tubulin. These results indicate that DFAT cells can transdifferentiate into neural cells, and contribute to the promotion of functional recovery. These findings also suggest that mature adipocytes could become a new source for cell replacement therapy to treat central nervous system disorders.

Research Products

• [Journal Article] Establishment and Characteristics of Porcine Preadipocyte Cell Lines Derived from Mature Adipocytes (2010)
  • Author(s): Nobusue H, Kano K
  • Journal Title: J Cell Biochem 109 Pages: 542-552
  • Peer Reviewed
• [Journal Article] Mature adipocyte-derived cells, dedifferentiated fat cells (DFAT), promoted functional recovery from spinal cord injury-induced motor dysfunction in rats. (2008)
  • Author(s): Ohta Y, Takenaga M, Tokura Y, Hamaguchi A, Matsumoto T, Kano K, Mugishima H, Okano H, Igarashi R
  • Journal Title: Cell Transplant 17 Pages: 877-86
  • Peer Reviewed
• [Journal Article] Mature Adipocyte Drived Dedifferentiated Fat Cells Exhabit Multilineage Potential (2008)
  • Author(s): Matsumoto T, Kano K, Kondo D, Fukuda N, Iribe Y, Tanaka N, Matsubara Y, Sakuma T, Satomi A, Otaki M, Ryu J, Mugishima H
  • Journal Title: 215 Pages: 210-222
  • Peer Reviewed
• [Journal Article] Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue. (2008)
  • Author(s): Nobusue H, Endo T, Kano K
  • Journal Title: Cell Tissue Res 332 Pages: 435-46
  • Peer Reviewed
• [Presentation] 成熟脂肪細胞に由来する多能性前駆細胞DFATの樹立と特性 (2009)
  • Author(s): 加野浩一郎
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟市
  • Year and Date: 2009-09-09
• [Presentation] 前駆脂肪細胞の分化程度は移植後の脂肪組織形成に影響を及ぼす (2008)
  • Author(s): 信末博行, 飯島明日香, 遠藤克, 加野浩一郎
  • Organizer: 日本畜産学会第109回大会
  • Place of Presentation: 水戸市
  • Year and Date: 2008-03-27
• [Presentation] GFPマウス成熟脂肪細胞に由来する前駆脂肪細胞株の樹立と脂肪組織形成 (2007)
  • Author(s): 信末博行, 遠藤克, 加野浩一郎
  • Organizer: 日本畜産学会第108回大会
  • Place of Presentation: 岡山市
  • Year and Date: 2007-09-26
• [Remarks]
  • URL: http://kenkyu-web.cin.nihon-u.ac.jp/Profiles/NA/0005684/profile.html