2008 Fiscal Year Final Research Report
A single nucleotide polymorphism genotyping method using a phosphate-binding tag nanomolecule
| Project/Area Number |
19590040
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
KINOSHITA Eiji Hiroshima University, 大学院・医歯薬学総合研究科, 准教授 (80304418)
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| Project Period (FY) |
2007 – 2008
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| Keywords | ゲノム / 遺伝子多型 / 一塩基多型(SNP) / リン酸 / 電気泳動 / フォスタグ(Phos-tag) |
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| Research Abstract |
研究代表者は, リン酸基に選択的な親和性をもつ機能性ナノ分子(フォスタグ, Phos-tag)を用いた新しい一塩基多型(SNP)検出法を開発した。この方法では, Phos-tagを共重合させた電気泳動用ポリアクリルアミドゲルを使用する。この方法により, ミニゲルクラスの電気泳動装置で, ほぼ100%の精度でSNPタイピングができる。本法が, 遺伝子多型の基礎研究分析や臨床診断に貢献できるものと期待している。
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Research Products
(30 results)
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[Journal Article] A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the dephosphorylation of phosphopeptides2009
Author(s)
Takiyama, K., Eiji Kinoshita, Kinoshita-Kikuta, E., Fujioka, Y., Kubo, Y., and Koike, T.
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Journal Title
Peer Reviewed
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[Journal Article] Synthetic ciguatoxins selectively activate NAv1. 8-derived chimeric sodium channels expressed in HEK293 cells2009
Author(s)
Yamaoka, K., Inoue, M., Miyazaki, K., Hirama, M., Kondo, C., Eiji Kinoshita, Miyoshi, H., and Seyama
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Journal Title
J. Biol. Chem. 284
Pages: 7597-7605
Peer Reviewed
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[Journal Article] A synthetic approach to develop peptide inhibitors selective for brain-type sodium channels on the basis of pompilidotoxin structure2009
Author(s)
Yokote, S., Setoguchi, R., Shimizu, E., Mishima, N., Kawahara, K., Kuniyasu, A., Shirasaki, T., Takahama, K., Konno, K., Kawai, N., Yamaoka, K., Eiji Kinoshita, and Nakayama, H.
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Journal Title
Heterocycles 79
Pages: 925-933
Peer Reviewed
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[Journal Article] Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes2009
Author(s)
Eiji Kinoshita, Kinoshita-Kikuta, E., Matsubara, M., Aoki, Y., Ohie, S., Mouri, Y., and Koike, T.
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Journal Title
Electrophoresis 30
Pages: 550-559
Peer Reviewed
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[Journal Article] FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway2008
Author(s)
Ishiai, M., Kitao, H., Smogorzewska, A., Tomida, J., Kinomura, A., Uchida, E., Saberi, A., Eiji Kinoshita, Kinoshita-Kikuta, E., Koike, T., Tashiro, S., Elledge, S.J., and Takata, M.
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Journal Title
Nat. Struct. Mol. Biol 15
Pages: 1138-1146
Peer Reviewed
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[Journal Article] Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE2008
Author(s)
Eiji Kinoshita, Kinoshita-Kikuta, E., Matsubara, M., Yamada, S., Nakamura, H., Shiro, Y., Aoki, Y., Okita, K., and Koike, T.
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Journal Title
Proteomics 8
Pages: 2994-3003
Peer Reviewed
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[Journal Article] Identification on membrane and characterization of phosphoproteins using an alkoxide-bridged dinuclear metal complex as a phosphate binding tag molecule2007
Author(s)
Nakanishi, T., Ando, E., Furuta, M., Eiji Kinoshita, Kinoshita-Kikuta, E., Koike, T., Tsunasawa, S., and Nishimura, O.
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Journal Title
J. Biomol. Tech. 18
Pages: 278-286
Peer Reviewed
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[Book] Single Nucleotide Polymorphisms
Author(s)
Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
Publisher
Zn(II)-Cyclen Polyacrylamide Gel Electrophoresis for SNP Detection(2d Edition, Chapter 11, Humana Press(in press))
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[Book] Single Nucleotide Polymorphisms
Author(s)
Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
Publisher
Phosphate-Affinity Polyacrylamide Gel Electrophoresis for SNP Genotyping(2d Edition, Chapter 11, Humana Press(in press))
