• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2008 Fiscal Year Final Research Report

Development of highly productive medicinal plants utilizing tissue-specific gene expression systems

Research Project

  • PDF
Project/Area Number 19590117
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionUniversity of Toyama

Principal Investigator

KUROSAKI Fumiya  University of Toyama, 大学院・医学薬学研究部(薬学), 准教授 (70143865)

Co-Investigator(Kenkyū-buntansha) YAMADA Kyoji  富山大学, 大学院・医学薬学研究部(薬学), 教授 (70200714)
LEE Jung-bum  富山大学, 大学院・医学薬学研究部(薬学), 助教 (40332655)
Project Period (FY) 2007 – 2008
Keywords薬用資源学 / 細胞内情報伝達系 / 遺伝子組み換え植物
Research Abstract

高等植物の二次代謝能の活性化にかかわる細胞内情報伝達系構成因子のうち、GTP結合タンパク質をコードする遺伝子を単離し器官特異的に過剰発現させて、物質生産への応用の可能性を検討した。ヘテロ三量体Gタンパクのαサブユニット遺伝子は刺激に対して殆んど応答しなかったが、単量体GTPase遺伝子の一部は転写レベルが上昇し、これを根組織で発現させた組み換え植物の分泌活性が顕著に上昇することを見出した。

  • Research Products

    (25 results)

All 2009 2008 2007

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (11 results)

  • [Journal Article] Cloning and characterization of a gene encoding Rac/Ro like monomeric guanosine 5'-triphosphate-binding protein from Scoparia dulcis2009

    • Author(s)
      Mitamura, T, Shite, M., Yamamura, Y., Kurosaki, F.
    • Journal Title

      Biol. Pharm. Bull 32( In press)

    • Peer Reviewed
  • [Journal Article] Cloning and characterization of Sdga gene encoding α-subunit of heterotrimeric GTP-binding protein complex in Scoparia dulcis2008

    • Author(s)
      Shite, M, Yamamura, Y., Hayashi, T, Kurosaki, F
    • Journal Title

      Biol. Pharm. Bull 31

      Pages: 2150-2153

    • Peer Reviewed
  • [Journal Article] Two RNA editing sites with cis-acting elements of moderate sequence identity are recognized by an identical site-recognition protein in tobacco chloroplasts2008

    • Author(s)
      Kobayashi, Y., Matsuo, M., Sakamoto, K., Wakasugi, T., Yamada, K, Obokata J
    • Journal Title

      Nucleic Acids Res 31

      Pages: 752-754

    • Peer Reviewed
  • [Journal Article] Effects of topical application of tacrolimus on acute itch-associated responses in mice2008

    • Author(s)
      Nakano T, Andoh T, Tayama M., Kosaka M., Lee J.-B, and Kuraishi Y
    • Journal Title

      Biol. Pharm. Bull 31

      Pages: 752-754

    • Peer Reviewed
  • [Journal Article] Different dorsal horn neurons responding to histamine and allergic itchstimuli2008

    • Author(s)
      Nakano T, Andoh T, Lee J.-B, Kuraishi Y.
    • Journal Title

      Neuroreport 19

      Pages: 723-726

    • Peer Reviewed
  • [Journal Article] Activation of proteinase-activated receptors induces itch associated response through histamine-dependent and independent pathways in mice2008

    • Author(s)
      Tsujii K, Andoh T, Lee J.-B, Kuraishi Y.
    • Journal Title

      J. Pharmacol. Sci 108

      Pages: 385-388

    • Peer Reviewed
  • [Journal Article] Enhanced secretory activity of Atropa belladonna hairy root culture over-expressing ADP-ribosylation factor gene2008

    • Author(s)
      Asakura, Y, Seki, H, Muranaka, T, Yamamura, Y, Kurosaki, F.
    • Journal Title

      Biol. Pharm. Bull 31

      Pages: 1465-1468

    • Peer Reviewed
  • [Journal Article] Structure and antiviral activity of an acidic polysaccharide from an edible blue-green alga2008

    • Author(s)
      Kanekiyo K, Hayashi K, Lee J.-B, Takenaka H, and Hayashi T
    • Journal Title

      Nostoc flagelliforme. Yakugaku Zasshi 128

      Pages: 725-731

    • Peer Reviewed
  • [Journal Article] Cloning and expression of Dcga gene encoding α-subunit of GTP binding protein in carrot seedlings2007

    • Author(s)
      Asakura, Y., Kurosaki, F
    • Journal Title

      30

      Pages: 1800-1804

    • URL

      Biol. Pharm. Bull

    • Peer Reviewed
  • [Journal Article] Cloning and expression of cDNAs encoding ADP-ribosylation factor in carrot seedling2007

    • Author(s)
      Asakura, Y, Ishigaki, E, Sugiyama, R., Kurosaki, F.
    • Journal Title

      Plant Science 172

      Pages: 189-195

    • Peer Reviewed
  • [Journal Article] Cloning and expression of calmodulin gene inScoparia dulcis.2007

    • Author(s)
      Saitoh, D, Asakura, Y, Kasidimoko, N, K, Shite, M, Sugiyama, R,, Lee, J.-B., Hayashi, T, Kurosaki F.
    • Journal Title

      Biol. Pharm. Bull. 30

      Pages: 1161-1163

    • Peer Reviewed
  • [Journal Article] Effect of partial desulfation and oversulfation of sodium spirulan on the potency of anti-herpetic activities2007

    • Author(s)
      Lee J.-B., Hou X., Hayashi K., Hayashi T.
    • Journal Title

      Carbohydr. Polym 69

      Pages: 651-658

    • Peer Reviewed
  • [Journal Article] Cordyceps militaris grown on germinated soybeans2007

    • Author(s)
      Ohta Y., Lee J.-B., Hayashi K., Fujita A., Park D.-K., Hayashi T.
    • Journal Title

      J. Agric. Food Chem 55

      Pages: 10194-10199

    • Peer Reviewed
  • [Journal Article] Anti-herpes virus target of an acidic polysaccharide, nostoflan, from an edible alga2007

    • Author(s)
      Kanekiyo K., Hayashi K., Takenaka H., Lee J.-B., Hayashi T.
    • Journal Title

      Nostoc fragelliforme. Biol. Pharm. Bull 30

      Pages: 1573-1575

    • Peer Reviewed
  • [Presentation] 海藻由来硫酸化多糖の生物活性2008

    • Author(s)
      李貞範, 林京子, 林利光
    • Organizer
      第17回天然薬物の開発と応用シンポジウム
    • Place of Presentation
      福岡
    • Year and Date
      20081113-14
  • [Presentation] ツルムラサキ(Basella rubra)由来抗ウイルス活性多糖に関する研究2008

    • Author(s)
      水越悠輔, 李貞範, 林京子, 林利光
    • Organizer
      日本生薬学会第55回年会
    • Place of Presentation
      長崎
    • Year and Date
      20080919-20
  • [Presentation] Methyl jasmonate influences secondary metabolism and protein expression in Scoparia dulcis2008

    • Author(s)
      3Lee J.-B., Suzuki S., Nkembo KM., Kayagi K., and Hayashi T.
    • Organizer
      7th Joint Meeting of GA, AFERP, ASP, PSE & SIF
    • Place of Presentation
      Athens, Greece
    • Year and Date
      20080803-08
  • [Presentation] Usefulness of algal polysaccharides for viral infectious diseases2008

    • Author(s)
      Ohta Y., Lee J.-B., Hayashi K., Takeshita A., and Hayashi T.
    • Organizer
      7th Joint Meeting of GA, AFERP, ASP, PSE & SIF
    • Place of Presentation
      Athens, Greece
    • Year and Date
      20080803-08
  • [Presentation] Anti-influenza A virus activity of an acidic polysaccharide from a blue-green alga Nostoc flagelliforme2008

    • Author(s)
      Hayashi K., Kanekiyo K., Ohta Y., Lee J.-B., Takenaka H., and Hayashi T
    • Organizer
      7th Joint Meeting of GA, AFERP, ASP, PSE & SIF
    • Place of Presentation
      Athens, Greece
    • Year and Date
      20080803-08
  • [Presentation] Signal transduction mechanisms involved in induced biosynthesis of defense-related compound in carrot2008

    • Author(s)
      Shite M, Yamamura Y, Kurosaki F
    • Organizer
      5th International Conference on Plant Metabolomics
    • Place of Presentation
      Yokohama
    • Year and Date
      20080715-18
  • [Presentation] Real-time analysis of cell wall degrading enzymes expression in plant-pathogenic fungus Fusarium verticillioides upon infection2008

    • Author(s)
      7Yamamura Y, Kurosaki F
    • Organizer
      5th International Conference on Plant Metabolomics
    • Place of Presentation
      Yokohama
    • Year and Date
      20080715-18
  • [Presentation] 緑藻ヒトエグサMonostroma nitidum由来硫酸化多糖の構造と生物活性2008

    • Author(s)
      李貞範, 小泉誠志, 林京子, 田中茂男, 林利光
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      20080326-28
  • [Presentation] 空心蓮子草Alternanthera philoxeroides由来ウイルス活性物質の作用特性2008

    • Author(s)
      Rattananthongkom Ariya, 李貞範, 林京子, 林利光
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      20080326-28
  • [Presentation] 褐藻イトヨレモクSargassum trichophyllum由来生物活性多糖の探索2008

    • Author(s)
      竹下亜澄, 李貞範, 林京子, 林利光
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      20080326-28
  • [Presentation] メチルジャスモン酸で誘導されるScoparia dulcisのジテルペン生合成活性化に関わる情報伝達関連遺伝子群のクローニング2008

    • Author(s)
      志手真人, 山村良美, 林利光, 黒崎文也
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      20080326-28

URL: 

Published: 2010-06-10   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi