2010 Fiscal Year Final Research Report
Studies on mechanism of the expression of aquaglyceroporins by insulin and contribution of aquaglyceroporins to glycerol metabolism
| Project/Area Number |
19590150
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
USUI Shigeyuki Gifu Pharmaceutical University, 薬学部, 准教授 (40176665)
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| Co-Investigator(Kenkyū-buntansha) |
HIRANO Kazuyuki 岐阜薬科大学, 薬学部, 教授 (90057365)
IGUCHI Kazuhiro 岐阜薬科大学, 薬学部, 助教 (10295545)
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| Project Period (FY) |
2007 – 2010
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| Keywords | アクアポリン3 / グリセロール / インスリン / エピネフリン / マグネシウム / トラフィッキング / プロテインキナーゼA / プロテインキナーゼC |
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| Research Abstract |
Expression and functional mechanisms of aquaporin (AQP) 3 was investigated using human colon carcinoma cell line (Caco-2). Forkhead box a2 (Foxa2) was suggested to be one of the transcriptional regulators for AQP3 gene expression mediated by insulin. Signal transducers, adenylyl cyclase, protein kinase A, mitogen-activated protein kinase kinase 1/2, and mitogen- and stress-activated protein kinase 1, were demonstrated to be involved in the signaling pathway for regulating transcription of the aquaporin 3 gene and cAMP response element-binding protein is one of the transcriptional regulators for aquaporin 3 gene expression mediated by magnesium ion. Protein kinase C was shown to modulate the externalization of AQP3 by epinephrine.
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