2009 Fiscal Year Final Research Report
The expression and function of double-stranded RNA-dependent protein kinase (PKR) in rat intestinal epithelial cells
| Project/Area Number |
19590201
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
DOI Yoshiaki University of Occupational and Environmental Health, Japan, 医学部, 教授 (30258602)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Hiroyuki 産業医科大学, 医学部, 准教授 (30335806)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 細胞分化 / 小腸上皮細胞 / アポトーシス / 二本鎖RNA依存性プロテインキナーゼ(PKR) / ハイドロコルチゾン / STAT-1 |
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| Research Abstract |
Intestinal epithelial cells (IECs) are exposed to microbial and viral products, and serve as essential barriers to them. Since double-stranded RNA-dependent protein kinase (PKR) is involved in cellular antiviral response, cell differentiation and apoptosis, we tried to investigate the expression and roles of PKR in rat IECs. In this study, we showed that the expression of PKR in IECs of adult rats. We also demonstrated that PKR was expressed in cultured rat IECs. The level of PKR protein expression and the activity of alkaline phosphatase (ALP) increased in the cultured IECs in a time-dependent manner. Treatment with PKR inhibitor decreased ALP activity in the IECs. The addition of synthetic double-stranded RNA induced apoptosis in a dose-dependent manner in these cells. Treatment with hydrocortisone also provoked suppression of PKR expression in such cells. Thus, we concluded that PKR is expressed in IECs as potent barriers to antigens and is a possible modulator of the differentiation and apoptosis in rat IECs.
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