• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2009 Fiscal Year Final Research Report

Mechanism of Nucleotide Binding Domain engine in ABC transporters

Research Project

  • PDF
Project/Area Number 19590215
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKeio University (2008-2009)
Osaka Medical College (2007)

Principal Investigator

SOHMA Yoshiro  Keio University, 医学部, 准教授 (60268183)

Co-Investigator(Kenkyū-buntansha) KUBOTA Takahiro  大阪医科大学, 医学部, 教授 (10084906)
SOHMIYA Koichi  大阪医科大学, 医学部, 助教 (20319544)
YAMAMOTO Daisuke  大阪医科大学, 医学部, 准教授 (50240106)
Research Collaborator HWANG T.-c  米ミズーリ大学, 医学部, 教授
Project Period (FY) 2007 – 2009
KeywordsABCトランスポータ / CFTR / チャネル / Nucleotide Binding Domain / ATP加水分解 / ゲーティング / アニオン / 二量体化
Research Abstract

Physiologically important ABC transporters share Nucleotide Binding Domain (NBD) to make a driving force for displaying their functions. From a series of functional data, we proposed a putative structure of the NBD engine of CFTR in one of the transitional states during its gating cycle. We also suggested a possibility that NBD engine utilized thermal motion energy of water molecules as well as ATP-hydrolysis energy. In addition, we obtained a reconstituted three dimensional structure of CFTR using a single particle analysis, which leads us to an upcoming direct observations of conformational changes of NBD engine.

  • Research Products

    (9 results)

All 2010 2009 2008

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (6 results)

  • [Journal Article] State-dependent modulation of CFTR gating by pyrophosphate.2009

    • Author(s)
      Tsai MF, Shimizu H, Sohma Y, Li M, Hwang TC
    • Journal Title

      J. Gen. Physiol. 133

      Pages: 405-419

    • Peer Reviewed
  • [Journal Article] Threedimensional reconstruction of human cystic fibrosis transmembrane conductance regulator chloride channel revealed an ellipsoidal structure with orifices beneath the putative transmembrane domain.2008

    • Author(s)
      Mio K, Ogura T, Mio M, Shimizu H, Hwang TC, Sato C, Sohma Y
    • Journal Title

      J. Biol. Chem. 283

      Pages: 30300-30310

    • Peer Reviewed
  • [Journal Article] Potentiation of BK channels by α5β1 integrin activation in arteriolar smooth muscle.2008

    • Author(s)
      Wu X, Yang Y, Gui P, Sohma Y, Meininger GA, Davis GE, Braun AP, Davis MJ
    • Journal Title

      J Physiol 586(6)

      Pages: 1699-1713

    • Peer Reviewed
  • [Presentation] olecular mechanism of a multi-functional Cl- channel CFTR. Symposium "Regulation of various physiological functions by chloridetransporting proteins"2010

    • Author(s)
      Sohma Y
    • Organizer
      87th Annual Meeting, The Physiological Society of Japan
    • Place of Presentation
      Morioka, Japan
    • Year and Date
      20100519-20100521
  • [Presentation] Functional Probing of an ABC Transpoter, Cystic Fibrosis Transmembraneconduc tance Regulator (CFTR) Channel by Small Aromatic Carboxylic Acids.2010

    • Author(s)
      Sohma Y, Nakamura Y, Yu YC, Furukawa T, Matsuzaki Y, Abe Y, Yasui M
    • Organizer
      83rd Annual Meeting of The Japanese Pharmacological Society
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      20100316-20100318
  • [Presentation] 3D STRUCTURE OF HUMNAN CFTR CHLORIDE CHANNEL REVEALED AN ELLIPSOIDAL FORM WITH ORIFICES BENEATH THE PUTATIVE TRANSMEMBRANE DOMAIN.2009

    • Author(s)
      Sohma Y, Mio K, Ogura T, Mio M, Shimizu H, Hwang TC, Sato C.
    • Organizer
      XXXVI International Congress of Physiological Sciences (IUPS2009) Function of Life: Elements and Integration
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20090727-20090801
  • [Presentation] Three dimensional reconstruction of CFTR chloride channel using single particle analysis.2009

    • Author(s)
      Mio K, Ogura T, Mio M, Shimizu H, Hwang TC, Sato C, Sohma Y
    • Organizer
      53th Biophysical Society Annual Meeting
    • Place of Presentation
      Boston, Massachusetts, USA
    • Year and Date
      20090228-20090304
  • [Presentation] Two distinct gating cycles of CFTR chloride channels.2009

    • Author(s)
      sai MF, Shimizu H, Sohma Y, Li M, Hwang TC.
    • Organizer
      53th Biophysical Society Annual Meeting
    • Place of Presentation
      Boston, Massachusetts, USA
    • Year and Date
      20090228-20090304
  • [Presentation] Mechanism of ATP hydrolysisdriven NBD gating engine in CFTR channel. Symposium "Dynamic Picture of Ion Channel upon Gating"2008

    • Author(s)
      Sohma Y
    • Organizer
      46th Annual Meeting, The Biophysical Society of Japan
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      20081203-20081205

URL: 

Published: 2011-06-18   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi