Analyses of molecular mechanisms underlying circadian rhythm generation and synchronization by rapid and efficient gene transduction in vivo

2008 Fiscal Year Final Research Report

• Project/Area Number: 19590235
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Kinki University
• Principal Investigator: HAYASAKA Naoto Kinki University, 医学部, 講師 (80368290)
• Co-Investigator(Renkei-kenkyūsha): NAKAJIMA Yoshihiro 独立行政法人産業技術総合研究所, セルエンジニアリング部門, 主任研究員 (10291080) ; AKASHI Makoto 佐賀大学, 医学部, 助教 (30398119)
• Project Period (FY): 2007 – 2008
• Keywords: 生体リズム

Research Abstract

GeneChipにより網羅的に採取した概日振動遺伝子群の中で、発現が組織特異的で且つ機能が特に注目される遺伝子改変動物を計7種類作製した。そのひとつRGS遺伝子の発現を抑制する2種の独立のノックダウンマウスを作製した。双方のマウスに共通して、活動リズムの周期変化、肝臓、膵臓の病理学的異常と糖代謝異常などを認めた。以上の結果より、概日時計に関与する新規のシグナル伝達系の存在が示唆された。

Research Products

• [Journal Article] MAP kinase is a functional component of the autonomous circadian system in the suprachiasmatic nucleus (2008)
  • Author(s): Hayasaka N, Akashi M, Yamazaki S, Node M
  • Journal Title: J Neurosci 28(18) Pages: 4619-23
  • Peer Reviewed
• [Journal Article] Modulatory effects of 5-fluorouracil on the rhythmic expression of arcadian clock genes : a possible mechanism of chemotherapy-induced arcadian rhythm disturbances (2008)
  • Author(s): Terazono H, Hamdan A, Matsunaga N, Hayasaka N, Kail H, Egawa T, Makino K, Smgeyoshi Y, Koyanagi S, Ohdo S
  • Journal Title: Biochem. Pharmacol 75(8) Pages: 1616-22
  • Peer Reviewed
• [Presentation] 3Dイメージングを用いた特定神経局在・ネットワーク解析への新たなアプローチ (2008)
  • Author(s): 早坂直人
  • Organizer: 日本時間生物学会
  • Place of Presentation: 岡山
  • Year and Date: 2008-11-09
• [Presentation] Three-dimensional imaging of Neural networks in brain : An approach for studying localization, interaction and function of specific neurons in a single circuit (2008)
  • Author(s): Hayasaka N
  • Organizer: 20^<th> Anniversary Meeting Society for Research on Biological Rhythms
  • Place of Presentation: Florida, USA
  • Year and Date: 2008-05-19
• [Presentation] Predominant expression of circadian-related genes in the SCN-denved astrocytes : implication of astrocyte functions in the circadian oscillator (2007)
  • Author(s): Hayasaka N
  • Organizer: 2^<nd> World Congress of Chronobiology
  • Place of Presentation: Tokyo
  • Year and Date: 2007-11-09