Mechanism of transformation of vascular smooth muscle cell-MicroRNA as regulating factor

2009 Fiscal Year Final Research Report

• Project/Area Number: 19590240
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Gunma University
• Principal Investigator: NAKAMURA Akio Gunma University, 大学院・医学系研究科, 講師 (30282388)
• Co-Investigator(Kenkyū-buntansha): KOHAMA Kazuhiro 群馬大学, 大学院・医学研究科, 教授 (30101116) ; ISHIKAWA Ryoki 群馬大学, 大学院・医学研究科, 講師 (20212863) ; OKAGAKI Tsuyoshi 三重大学, 大学院・生物資源学科, 教授 (80185412) ; KISHI Hiroko 山口大学, 大学院・医学研究科, 講師 (40359899)
• Project Period (FY): 2007 – 2009
• Keywords: 血管平滑筋 / 細胞分化 / 脱分化 / マイクロRNA / 細胞遊走

Research Abstract

The micro RNA (miRNA) of non-coding RNA bind to a target mRNA after transcription of the gene and restrain the translation. It has been clarified that this gene silencing mechanism is processed among various species from plant to mammalian widely. Especially, it is thought that miRNA plays an important role on the way of the cell differentiation. The regulation of gene expression for two phenotypes (contraction and proliferation) of vascular smooth muscle cell (VSMC) is not well known. We established that the platelet-derived growth factor (PDGF) and sodium butyrate (NaB) stimulated de-differentiation and differentiation of VSMC, respectively. The regulation of gene expression required for the above two phenotypic modulations is not well known. We found the up-regulated miRNA by using microarray from each two phenotype of VSMC to induce with these medicines. The two up-regulated miRNA according to the de-differentiation stimulated the cell migration.

Research Products

• [Journal Article] Phosphatase coincident with cessation of cytoplasmic streaming in Physarum polycephalum (2010)
  • Author(s): Okada, C., Nakamura, A., Tomioka, S., Kohama, K., Kaneko, T. S.
  • Journal Title: Cell Biol.Int. (impress)
  • Peer Reviewed
• [Journal Article] Calcium wave for cytoplasmic streaming of Physarum polycephalum (2010)
  • Author(s): Yoshiyama, S., Ishigami, M., Nakamura, A., Kohama, K.
  • Journal Title: Cell Biol. Int. 34 Pages: 35-40
  • Peer Reviewed
• [Journal Article] Stimulatory effects of arachidonic acid on myosin ATPase activity and contraction of smooth muscle via myosin motor domain (2010)
  • Author(s): Katayama, T., Watanabe, M., Tanaka, H., Hino, M., Miyakawa, T., Ohki, T., Ye, L-H., Xie, C., Yoshiyama, S., Nakamura, A., Ishikawa, R., Tanokura, M., Oiwa, K., Kohama, K.
  • Journal Title: Am.J.Physiol.Heart Circ.Physiol. 298 Pages: H1505-514
  • Peer Reviewed
• [Journal Article] Calcium regulation of non-kinase and kinase activities of recombinant myosin light-chain kinase and its mutants (2009)
  • Author(s): Xie, C., Zhang, Y., Wang, H., Matsumoto, A., Nakamura, A., Ishikawa, R., Yoshiyama, S., Hayakawa, K., Kohama, K., Gao, Y.
  • Journal Title: IUBMB Life 61 Pages: 1092-1098
  • Peer Reviewed
• [Journal Article] Non-kinase Activity of MLCK in Elongated Filopodia Formation and Chemotaxis of Vascular Smooth Muscle Cells toward Sphingosylphosphorylcholine (2009)
  • Author(s): Wang, H-H., Nakamura, A., Matsumoto, A., Yoshiyama, S., Qin, X., Ye, LH., Xe, C., Zhang, Y., Gao, Y., Ishikawa, R., Kohama, K.
  • Journal Title: Am.J.Physiol.Heart Circ.Physiol. 296 Pages: H1683-1693
  • Peer Reviewed
• [Journal Article] The non-kinase activity of myosin light chain kinase in regulating smooth muscle contraction (2009)
  • Author(s): Nakamura, A.
  • Journal Title: Nippon Yakurigaku Zasshi. 133 Pages: 144-148
• [Journal Article] Plectin 1 links intermediate filaments to costameric sarcolemma through β?synemin, α-dystrobrevin and actin (2008)
  • Author(s): Hijikata, T., Nakamura, A., Isokawa, K., Imamura, M., Yuasa K., Ishikawa, R., Kohama, K., Takeda, S., Yorifuji, H.
  • Journal Title: J.Cell Sci. 121 Pages: 2062-2074
  • Peer Reviewed
• [Journal Article] Blebbistatin Inhibits the Chemotaxis of Vascular Smooth Muscle Cells through Breaking the Interaction between Myosin II and Actin (2008)
  • Author(s): Wang, H-H., Tanaka, H., Qin, X., Zhao, T., Ye, L., Okagaki, T., Katayama, T., Nakamura, A., Ishikawa, R., Thatcher, S., Wright, G., Kohama, K.
  • Journal Title: J.Physiol.Heart Circ. Physiol. 294 Pages: H2060-H2068
  • Peer Reviewed
• [Journal Article] Role of non-kinase activity of myosin light- chain kinase in regulating smooth muscle contraction, a review dedicated to Dr. Setsuro Ebashi (2008)
  • Author(s): Nakamura, A., Xie, C., Zhang, Y., Gao, Y., Wang, H-H., Ye, L-H., Kishi, H., Okagaki, T., Yoshiyama, S., Hayakawa, K., Ishikawa, R., Kohama, K.
  • Journal Title: Biochem.Biophys.Res.Commun. 369 Pages: 135-43
  • Peer Reviewed
• [Journal Article] Drebrin attenuates the interaction between actin and myosin-V. (2007)
  • Author(s): Ishikawa, R., Katoh, K., Takahashi, A., Xie, C., Oseki, K., Watanabe, M., Igarashi, M., Nakamura, A., Kohama K.
  • Journal Title: Biochem.Biophys.Res.Commun. 359 Pages: 398-401
  • Peer Reviewed
• [Journal Article] Identification of substrates for transglutaminase in Physarum polycephalum, an acellular slime mold, upon cellular mechanical damage (2007)
  • Author(s): Wada. F., Hasegawa, H., Nakamura, A., Sugimura, Y., Kawai, Y., Sasaki, N., Shibata, H., Maki, M., Hitomi, K.
  • Journal Title: FEBS J. 274 Pages: 2766-2777
  • Peer Reviewed
• [Presentation] The novel calmodulin-binding site of the smooth muscle myosin light chain kinase (2010)
  • Author(s): Nakamura, A., Matsumoto, A., Xie, C., Yoshiyama, S., Ishikawa, R., Kohama, K.
  • Organizer: The 83^<rd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Osaka
  • Year and Date: 2010-03-16
• [Presentation] Insulin signaling of diabetic mother rats and their offspring (2010)
  • Author(s): Nasu, R., Kohama, T., Nakamura, A., Kohama, K.
  • Organizer: The 83^<rd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Osaka
  • Year and Date: 2010-03-16
• [Presentation] Calcium regulation of the ATPase activities of scallop and physarum myosins : role of essential light chain (2010)
  • Author(s): Zhang Y., Nakamura A., Kawamichi, H., Kohama K.
  • Organizer: The 83^<rd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Osaka
  • Year and Date: 2010-03-16
• [Presentation] 非筋細胞ミオシンIIの10S構造形成に必要な尾部領域の探索 (2010)
  • Author(s): 木卜貴之, 中村彰男, 矢澤道生, 村上洋太, 高橋正行
  • Organizer: 2010年生体運動研究合同班会議
  • Place of Presentation: 東京(中央大学)
  • Year and Date: 2010-01-09
• [Presentation] Structural and function of Physarum CBP40 (2009)
  • Author(s): Nakamura A.
  • Organizer: 21^<th> IUBMB International Congress of Biochemistry and Molecular Biology and 12^<th> FAOBMB Congress
  • Place of Presentation: Shanghai, China
  • Year and Date: 20090802-20090807
• [Presentation] Calcium regulation of non-kinase and kinase activities of myosin light-chain kinase (2009)
  • Author(s): Zhang Y., Xie C., Matsumoto, A., Nakamura A., Zhao, Y., Kohama K., Gao Y.
  • Organizer: 21^<th> IUBMB International Congress of Biochemistry and Molecular Biology and 12^<th> FAOBMB Congress
  • Place of Presentation: Shanghai, China
  • Year and Date: 20090802-20090807
• [Presentation] 平滑筋ミオシン軽鎖キナーゼの新しい機能の研究 (2009)
  • Author(s): 謝策, 中村彰男, 小濱一弘
  • Organizer: 第32回日本バイオレオロジー学会年会
  • Place of Presentation: 桐生市市民文化会館
  • Year and Date: 20090604-20090605
• [Presentation] Non-kinase activity of MLCK in inducing filopodia and chemotaxis of vascular smooth muscle cells toward sphingosylphosphoryl- choline (2009)
  • Author(s): Wang H.-H., Nakamura A., Matsumoto A., Yoshiyama S., Zin X., Ye L.-H., Xie C., Zhang Y., Gao Y., Ishikawa R., Kohama K.
  • Organizer: The 82^<nd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20090316-20090318
• [Presentation] Cell motility of vascular smooth muscel cells exposed to the cigarette smoke components (2009)
  • Author(s): Yoshiyama S., Wang H.-H., Nakamura A., Kohama K.
  • Organizer: The 82^<nd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20090316-20090318
• [Presentation] Sliding of F-actin on myosin II under the conditions fully saturated with variety of actin-binding proteins (2009)
  • Author(s): Ishikawa R., Takahashi A., Nakamura A., Kohama K.
  • Organizer: The 82^<nd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20090316-20090318
• [Presentation] The analysis of calcium inhibition by constructing hybrid myosins between physarum and smooth muscle myosins (2009)
  • Author(s): Zhang Y., Kawamichi H., Nakamura A., Nyitray L., Kohama K.
  • Organizer: The 82^<nd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20090316-20090318
• [Presentation] 種々のアクチン結合蛋白質が飽和結合する条件での in vitro motility assay (2009)
  • Author(s): 石川良樹, 中村彰男, 小浜一弘
  • Organizer: 2009年生体運動研究合同班会議
  • Place of Presentation: 東京
  • Year and Date: 20090109-20090111
• [Presentation] Role of Essential Light Chain in Regulating the ATPase Activities of Scallop and Physarum Myosins (2009)
  • Author(s): Zhang Y., Nakamura A., Kawamichi, H., Kohama K.
  • Organizer: 49^<th> American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2009-12-06
• [Presentation] Characterization of the non-kinase activity of smooth muscle myosin light chain kinase (2008)
  • Author(s): Xie C., Nakamura A., Zhang Y., Gao Y., Ishikawa R., Yoshiyama S., Kohama K.
  • Organizer: 48^<th> annual meeting of the American Society for Cell Biology
  • Place of Presentation: San Francisco (Moscone Center)
  • Year and Date: 20081213-20081217
• [Presentation] A novel phosphatase from Physarum polycephalum III (2008)
  • Author(s): Okada C., Nakamura A., Yoshiyama S., Kohama K., Kaneko T.
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学大会合同大会
  • Place of Presentation: 神戸(神戸ポートアイランド)
  • Year and Date: 20081209-20081212
• [Presentation] Actin-bundles as a transporter of nano-sized cargoes (2008)
  • Author(s): Yoshiyama S., Ishikawa R., Nakamura A., Higashi-Fujime S., Takatsuki H., Rice K., Blough E. R., Kohama K.
  • Organizer: 2^<nd> International Symposium on Bio-nano- systems
  • Place of Presentation: Tokyo(東京大学)
  • Year and Date: 20081031-20081102
• [Presentation] In vitro sliding on myosin II of F-actin under the conditions fully saturated with variety of actin-binding proteins (2008)
  • Author(s): Ishikawa R., Takahashi A., Nakamura A., Kohama K.
  • Organizer: 2^<nd> International Symposium on Bio-nano-systems
  • Place of Presentation: Tokyo(東京大学)
  • Year and Date: 20081031-20081102
• [Presentation] Calcium inhibition of Physarum myosin in associated with its conformational change (2008)
  • Author(s): Zhang Y., Nakamura A., Yoshiyama S., Kohama K., Kawamichi H., Farkas L., Nyitray L.
  • Organizer: 2008第55回 北関東医学会総会
  • Place of Presentation: 群馬
  • Year and Date: 20080925-20080926
• [Presentation] The non-kinase activity of myosin light chain kinase in regulating smooth muscle contraction (2008)
  • Author(s): Nakamura A., Xie C., Matsumoto A., Zhang Y., Gao Y., Kohama K.
  • Organizer: 10^<th> Meeting of the European Calcium Society
  • Place of Presentation: Leuven, Belgium
  • Year and Date: 20080917-20080920
• [Presentation] 平滑筋ミオシン軽鎖キナーゼの非キナーゼ活性 (2008)
  • Author(s): 中村彰男, 松本篤, 謝策, 張月, 吉山伸司, 小浜一弘
  • Organizer: 第79回日本動物学会年会
  • Place of Presentation: 福岡(福岡大学)
  • Year and Date: 20080905-20080907
• [Presentation] 質量分析装置を用いたミオシン軽鎖キナーゼのリン酸化部位の決定 (2008)
  • Author(s): 中村彰男, 岸博子, 松本篤, 石川良樹, 吉山伸司, 早川晃一, 小林誠, 小濱一弘
  • Organizer: 第118回薬理学会関東部会・第10回応用薬理シンポジウム
  • Place of Presentation: 東京(星薬科大)
  • Year and Date: 20080607-20080608
• [Presentation] The regulatory effect of Ca^<2+>/calmodulin on the actin-binding activity of smooth muscle myosin light chain kinase (2008)
  • Author(s): Zhang Y., Xie C., Nakamura A., Gao Y., Ishikawa R., Yoshiyama S., Matsumoto A., Kohama K.
  • Organizer: The 81^<st> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20080317-20080319
• [Presentation] Expression and characterization of the kinase-dead mutants of myosin light chain kinase (2008)
  • Author(s): Xie C., Nakamura A., Zhang Y., Gao Y., Kawano K., Ishikawa R., Matsumoto A., Tanaka H., Kohama K.
  • Organizer: The 81^<st> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20080317-20080319
• [Presentation] Dynamics of actomyosin and intracellular Ca^<2+> during cell movement, in case of Physarum polycephalum (2008)
  • Author(s): Yoshiyama S., Ishigami M., Kohama K.
  • Organizer: The 81^<st> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20080317-20080319
• [Presentation] Non-kinase activity of MLCK in elongated filopodia formation and chemotaxis of vascular smooth muscle cells in response to sphingosylphosphorylcholine (2008)
  • Author(s): Wang H-H., Nakamura A., Matsumoto A., Hayakawa K., Yoshiyama S., Qin X., Tanaka H., Ye L., Li S., Ishikawa R., Kohama K.
  • Organizer: The 81^<st> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20080317-20080319
• [Presentation] Arachidonic acid binds to heavy chain of smooth muscle myosin and stimulates ATPase activity (2008)
  • Author(s): Katayama T., Miyakawa T., Yoshiyama S., Nakamura A., Tanaka H., Tanokura M., Kohama K.
  • Organizer: The 81^<st> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20080317-20080319
• [Presentation] Brain specific actin-binding protein Drebrin inhibits the cofilin-dependent actin dynamics (2008)
  • Author(s): Ishikawa R., Katoh K., Ebihara S., Takahashi A., Igarashi M., Nakamura A., Kohama K.
  • Organizer: The 81^<st> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20080317-20080319
• [Presentation] Screening of phenotype-specific genes of vascular smooth muscle cell (VSMC) and their characterization (2008)
  • Author(s): Miyamoto H., Nagahama R., Nakamura A., Kohama K., Okagaki T.
  • Organizer: The 81^<st> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20080317-20080319
• [Presentation] The non-kinase activity of myosin light chain kinase in regulating smooth muscle contraction (2008)
  • Author(s): Nakamura A.
  • Organizer: The 81^<st> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama
  • Year and Date: 20080317-20080319
• [Presentation] Blebbistatin inhibits the chemotaxis of vascular smooth muscle cells through breaking the interaction between smooth muscle myosin II and actin (2008)
  • Author(s): Wang H-H., Tanaka H., Qin X., Zhao T., Lihong Ye, Okagaki T., Katayama T., Nakamura A., Ishikawa R., Thatcher S. E., Wright G. L., Kohama K.
  • Organizer: The Joint Biophysical Society 52^<nd> Annual Meeting and 16th International Biophysics Congress
  • Place of Presentation: California
  • Year and Date: 20080202-20080206
• [Presentation] ドレブリンによるコフィリン活性阻害 (2008)
  • Author(s): 石川良樹, 海老原利枝, 加藤薫, 五十嵐道弘, 中村彰男, 小浜一弘
  • Organizer: 2008年生体運動研究会合同班会議
  • Place of Presentation: 仙台
  • Year and Date: 20080107-20080109
• [Presentation] Drebrin による ADF/cofilin 活性阻害 (2007)
  • Author(s): 石川良樹, 加藤薫, 高橋あゆみ, 中村彰男, 小浜一弘
  • Organizer: 第45回 日本生物物理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20071221-20071225
• [Presentation] A novel phosphatase from Physarum polycephalum II. (2007)
  • Author(s): Okada, C., Nakamura, A., Yoshiyam, S., Kohama, K., Kaneko, K.
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 20071211-20071215
• [Presentation] 培養血管平滑筋細胞を使った形質変換特異的遺伝子のスクリーニング (2007)
  • Author(s): 長濱良治, 宮本洋行, 中村彰男, 小濱一弘, 岡垣壮
  • Organizer: 15^<th> 日本血管生物医学会学術大会
  • Place of Presentation: Fukuoka
  • Year and Date: 20071129-20071130
• [Presentation] セリン・スレオニンキナーゼによるコンビナントミオシン軽鎖キナーゼのリン酸化部位の決定 (2007)
  • Author(s): 中村彰男, 岸博子, 松本篤, 小林誠, 小濱一弘
  • Organizer: 15^<th> 日本血管生物医学会学術大会
  • Place of Presentation: Fukuoka
  • Year and Date: 20071129-20071130
• [Presentation] Determination of phosphorylation site of smooth muscle myosin light chain kinase by various Ser/Thr kinases (2007)
  • Author(s): Nakamura, A., Kishi, H., Matsumoto, A., Ishikawa, R., Kobayashi, S., Kohama, K.
  • Organizer: Joint meeting of the 40th Japanese Society of Developmental Biologists & the 59th Japan Society for Cell Biology
  • Place of Presentation: Fukuoka
  • Year and Date: 20070528-20070530
• [Presentation] ドレブリンはアクチン細胞骨格流動促進因子コフィリンの活性を阻害する (2007)
  • Author(s): 石川良樹, 中村彰男, 小浜一弘
  • Organizer: 第117回薬理学会関東部会
  • Place of Presentation: 東京
  • Year and Date: 2007-10-06
• [Book] Int.Rev.Cell Mol.Biol. 276(Cell and molecular biology of the fastest myosins) (2009)
  • Author(s): Higashi-Fujime, S., Nakamura, A.
  • Total Pages: 301-347