2009 Fiscal Year Final Research Report
Angiogenic potential of multipotent neural stem cells
| Project/Area Number |
19590262
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Meiji Pharmaceutical University |
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Principal Investigator |
OISHI Kazuhiko Meiji Pharmaceutical University, 薬学部, 教授 (80203701)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 神経幹細胞 / 内皮細胞 / 血管腔構造 / 三次元培養 / 分化 / 脳血管 / 平滑筋細胞 |
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| Research Abstract |
We found that CD44^+ CD90^+ cells isolated from primary neurospheres can form vascular tube structures in vitro. NSCs isolated from the mouse embryonic cortex formed neurospheres when cultured in serum-free medium containing 20ng/ml basic fibroblast growth factor (bFGF). CD44^+ CD90^+ cells were enriched from the neurospheres using an EPICS ALTRA flow cytometer, and antibodies against CD44 and CD90. The purified CD44^+ CD90^+ cells generated neurospheres, and differentiated into neurons and astrocytes. When the cells were inoculated into collagen gels and cultured with 20% fetal bovine serum plus bFGF for 7 d, vascular tube-like structures were formed. These results indicate that CD44^+ CD90^+ cells have the ability to generate neurospheres and to form vascular tubes.
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