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2008 Fiscal Year Final Research Report

Analysis of physiological function of vaults

Research Project

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Project/Area Number 19590314
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKagoshima University

Principal Investigator

AKIYAMA Shinichi  Kagoshima University, 大学院・医歯学総合研究科, 教授 (60117413)

Co-Investigator(Kenkyū-buntansha) CHE Xiao-fang  鹿児島大学, 大学院・医歯学総合研究科, 助教 (10437973)
Project Period (FY) 2007 – 2008
KeywordsMVP / Vault / LRP / osmotic stress / PI3K / Akt
Research Abstract

Vaultは、細胞内で一番大きなリボ核蛋白体であるが、その生理機能は現在まで不明である。今回の研究で、大腸癌細胞に高浸透圧ストレスを加えると、MVPの発現が亢進すること、MVPが発現した細胞は、高浸透圧に対して耐性になることを見出した。
MVP の発現は、腎臓や腸管などの恒常的に浸透圧変化の大きい組織において認められている。腎臓の腎髄質細胞は、高濃度のNaClにさらされ、500mM以上の塩濃度になることもある。高い塩濃度はアポトーシスを誘導するが、細胞はvaultの発現を亢進することにより、高浸透圧によって誘導されるアポトーシスを回避している可能性がある。

  • Research Products

    (38 results)

All 2009 2008 2007 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (20 results) Book (2 results)

  • [Journal Article] Molecular basis for the induction of an angiogenesis inhibitor, thrombospondin-1, by 5-FU2008

    • Author(s)
      Zhao H-Y, Che X-F, Akiyama S(他12名)
    • Journal Title

      Cancer Res 68

      Pages: 7035-7041

    • Peer Reviewed
  • [Journal Article] Hyperosmotic Stress Up-regulates the Expression of Major Vault Protein in SW620 Human Colon Cancer Cells. Exp2008

    • Author(s)
      Ikeda R, Che X-F, Akiyama S(他16名)
    • Journal Title

      Cell Res 314

      Pages: 3017-3026

    • Peer Reviewed
  • [Journal Article] Down regulation of c-Myc and induction of an angiogenesis inhibitor, thrombospondin-1, by 5-FU in KM12C cells2008

    • Author(s)
      Zhao H-Y, Che X-F, Akiyama S(他12名)
    • Journal Title

      Cancer Lett. 270

      Pages: 156-163

    • Peer Reviewed
  • [Journal Article] Copper Transport Systems are Involved in Multidrug Resistance and Drug Transport2008

    • Author(s)
      Furukawa T, Akiyama S(他3名)
    • Journal Title

      Curr Med Chem 15

      Pages: 3268-3278

    • Peer Reviewed
  • [Journal Article] Suppression of the proliferation of cancer cell lines, KB-3-1 and K562 cells preceded by a decrease in intracellular pH caused by phenoxazine derivatives2008

    • Author(s)
      Che X-F, Akiyama S, Tomoda A
    • Journal Title

      Oncol Rep 19

      Pages: 1253-1258

    • Peer Reviewed
  • [Journal Article] Thymidine phosphorylase inhibits the expression of proapoptic protein BNIP32008

    • Author(s)
      Ikeda R, Che X-F, Akiyama S(他13名)
    • Journal Title

      Biochem Biophys Res Commun 370

      Pages: 220-224

    • Peer Reviewed
  • [Journal Article] Anti-angiogenic effect of 5-Fluorouracil-based drugs against human colon cancer xenografts2008

    • Author(s)
      Ooyama A, Akiyama S(他4人)
    • Journal Title

      Cancer Lett 267

      Pages: 26-36

    • Peer Reviewed
  • [Journal Article] Apoptosis induction preceded by mitochondrial depolarization in multiple myeloma cell line U266 by 2-aminophenoxazine-3-one2008

    • Author(s)
      Shirato K, Che X-F, Akiyama S(他5人)
    • Journal Title

      Biol Pharm Bull 31

      Pages: 62-67

    • Peer Reviewed
  • [Journal Article] Alterations in the glycoform of cisplatin-resistant human carcinoma cells are caused by defects in the endoplasmic reticulum-associated degradation system2008

    • Author(s)
      Nakagawa H, Akiyama S(他11人)
    • Journal Title

      Cancer lett 270(2)

      Pages: 295-301

    • Peer Reviewed
  • [Journal Article] Copper-Transporting P-Type ATPase, ATP7A, Confers Multidrug Resistance and Its Expression Is Related to Resistance to SN-38 in Clinical Colon Cancer2007

    • Author(s)
      Owatari S, Che X-F, Akiyama S(他12人中)
    • Journal Title

      Cancer Res 67

      Pages: 4860-4868

    • Peer Reviewed
  • [Journal Article] Reversal of P-Glycoprotein-Mediated Multidrug Resistance by Sipholane Triterpenoids2007

    • Author(s)
      Jain S, Akiyama S(他6人)
    • Journal Title

      J. Nat. Prod 70

      Pages: 928-931

    • Peer Reviewed
  • [Journal Article] Involvement of Mitogen-activated protein kinase signaling pathways in microcystin-LR-induced apoptosis after its selective uptake mediated by OATP1B1 and OATP1B3 : microcystin-lr induces the apoptosis via mapk2007

    • Author(s)
      Komatsu M, Akiyama S(他6人)
    • Journal Title

      Toxicol. Sci 97

      Pages: 407-416

    • Peer Reviewed
  • [Journal Article] ABCB6 Localizes toboth the outer mutocondrial membrane and the plasma membrane2007

    • Author(s)
      Jill K. Paterson, Akiyama S(他11人)
    • Journal Title

      Biochemistry 33

      Pages: 9443-9452

    • Peer Reviewed
  • [Journal Article] Reversal effect of BM-cyclin 1 on multidrug resistance in C-A120 cells2007

    • Author(s)
      Wang L, Akiyama S(他4人)
    • Journal Title

      Anti-cancer Drug 18

      Pages: 1015-1021

    • Peer Reviewed
  • [Journal Article] Isolation and characterization of arsenite-resistant human epidermoid carcinoma KB cells2007

    • Author(s)
      Tachiwada T, Che X-F, Akiyama S(他10人)
    • Journal Title

      Oncol Rep 18

      Pages: 721-727

    • Peer Reviewed
  • [Journal Article] Cepharanthine is a potent reversal agent for MRP7 (ABGG10)-mediated multidrug resistance

    • Author(s)
      Ying Zhou, Shin-ichi Akiyama(他11人)
    • Journal Title

      Biochem Pharmacol (in press)

    • Peer Reviewed
  • [Presentation] Gemcitabinenの耐性と核酸輸送蛋白質第31回日本分子生物学会年会2008

    • Author(s)
      古川龍彦
    • Organizer
      第81回日本生化学大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20081200
  • [Presentation] チミジンホスホリラーゼ発現腫瘍細胞におけるNF-κBを介したIL-8の発現亢進機講2008

    • Author(s)
      田畑祥
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議
    • Year and Date
      2008-10-29
  • [Presentation] ヒト大腸癌における5-FUによる血管新生阻害因子TSP-1の誘導2008

    • Author(s)
      趙紅業
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-29
  • [Presentation] 膵癌細胞株MIAPaCa-2由来Gemcitabine耐性細胞の耐性機構の解析2008

    • Author(s)
      古川龍彦
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-29
  • [Presentation] survivinペプチド(89-104アミノ酸)によるATL細胞のアポトーシス誘導2008

    • Author(s)
      車暁芳
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-29
  • [Presentation] 低酸素によるヒト線維芽細胞でのPGISの高発現2008

    • Author(s)
      王嘉
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-29
  • [Presentation] 腫瘍微小血管に対する5-FUとその活性化酵素の作用2008

    • Author(s)
      秋山伸一
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-28
  • [Presentation] p53R2標的siRNAは悪性黒色の腫瘍増殖を抑制して抗癌剤感受性を高める2008

    • Author(s)
      松下茂人
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-28
  • [Presentation] 膵癌細胞株MIAPaCa-2由来Gemcitabine耐性細胞の耐性機構の解析2008

    • Author(s)
      古川龍彦
    • Organizer
      第12回がん分子標的治療研究会総会
    • Place of Presentation
      学術総合センター(東京)
    • Year and Date
      2008-06-27
  • [Presentation] 第29回癌免疫外科研究会2008

    • Author(s)
      秋山伸一
    • Place of Presentation
      ホテル日航東京
    • Year and Date
      2008-06-19
  • [Presentation] 籃藻毒マイクロシスチンLRの肝臓特異的毒性の発現機構第30回日本分子生物学学会年会・第80回日本生化学大会2007

    • Author(s)
      小松正治
    • Organizer
      合同大会
    • Place of Presentation
      パシフィコ横浜ヨコハマグランドインンターコンチネンタルホテル
    • Year and Date
      2007-12-14
  • [Presentation] 銅輸送体ATP7A発現による抗癌剤耐性の機構第30回日本分子生物学学会年会・第80回日本生化学大会2007

    • Author(s)
      古川龍彦
    • Organizer
      合同大会
    • Place of Presentation
      パシフィコ横浜ヨコハマグランドインンターコンチネンタルホテル
    • Year and Date
      2007-12-11
  • [Presentation] Anti-angiogenic effect of 5-fluorouacil based drug against human colon cancer xenograft2007

    • Author(s)
      Akio Ooyama
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-10-05
  • [Presentation] Thymidine phosphorylase suppresses the expression of proapoptic protein BNIP32007

    • Author(s)
      Ryuji Ikeda
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-10-05
  • [Presentation] The effect of thymidine phosphorylase, 5-FU and hypoxia on tumor microenvironments.(シンポジウム)2007

    • Author(s)
      Shin-chi Akiyama
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-10-04
  • [Presentation] A copper transporter ATP7A mediate multidrug resistance and the vesicle transport system2007

    • Author(s)
      Tatsuhiko Furukawa
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-10-04
  • [Presentation] Induction of angiogenesis inhibitor thrombospondin-1 by 5-FU in human colon cancer cells2007

    • Author(s)
      Hong-Ye Zhao
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-10-03
  • [Presentation] Increased expression of PGIS in two human fibroblast cell lines under hypoxic condition2007

    • Author(s)
      Jia Wang
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-10-03
  • [Presentation] Molecular basis for the induction of interleukin-8 by thmidine phosphorylase2007

    • Author(s)
      Sho Tabata
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-10-03
  • [Presentation] The effect of hypoxia on gene expression in human fibroblast WI-38 cells2007

    • Author(s)
      Xiao-Fang Che
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-10-03
  • [Book] 日本臨床がん薬物療法学(増刊号)2009

    • Author(s)
      秋山伸一
  • [Book] 消化器がん化学療法2008

    • Author(s)
      秋山伸一
    • Publisher
      日本メディカルセンター

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Published: 2010-06-10   Modified: 2016-04-21  

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