腫瘍に対する免疫監視機構におけるDNAM-1の機能の解明

2007 Fiscal Year Annual Research Report

• Project/Area Number: 19590382
• Research Institution: University of Tsukuba
• Principal Investigator: 田原 聡子 University of Tsukuba, 大学院・人間総合科学研究科, 助教 (20360589)
• Keywords: DNAM-1 / CD226 / CD155 / CD112 / 免疫監視 / 腫瘍 / メチルコラントン / 化学癌誘発剤

Research Abstract

【目的】
DNAM-1は細胞傷害性T細胞およびNK細胞に強く発現しており、腫瘍細胞などに発現するリガンド(CD155またはCD112)を認識して接着し、腫瘍細胞に細胞傷害を誘導する。本研究では腫瘍に対する免疫監視におけるDNAM-1の機能を明らかにするため、DNAM-1欠損マウスを用いて解析した。
【方法・結果】
化学癌誘発剤(メチルコラントレン: MCA)による腫瘍の発生に対する免疫監視機構におけるDNAM-1の機能について検討した。MCA25μgを皮下に接種すると、DNAM-1欠損マウスでは腫瘍の発生率が野生型マウスと比較して有意に亢進し、生存率が低下することを明らかにした。
【研究意義】
DNAM-1は腫瘍の発生に対する免疫監視機構において重要な役割を有することが示された。

Research Products

• [Journal Article] Expression of a Splicing Isoform of MAIR-V(CD300LF), an inhibitory immunoglobulin-like receptor on myeloid cells. (2008)
  • Author(s): Can I, et. al.
  • Journal Title: Hybridoma 27:1 Pages: 59-61
  • Peer Reviewed
• [Journal Article] Caspase-independent cell death by CD300LF(MAIR-V), an inhibitory immunoglobulin-like receptor on myeloid cells (2008)
  • Author(s): Can I, et. al.
  • Journal Title: Journal of Immunology 180:1 Pages: 207-13
  • Peer Reviewed
• [Journal Article] Activation of neutropbils by a novel thiggering immunoglobulin-like receptor MAIR-IV. (2007)
  • Author(s): Nakano T, et. al.
  • Journal Title: Molecular immunology 45(1) Pages: 289-94
  • Peer Reviewed
• [Journal Article] Dual assemblies of an activating immune receptor, MAIR-II, With ITAM-bearing adapters DAP12 and FcR chain on peritoneal macrophages. (2007)
  • Author(s): Nakahashi C, et. al.
  • Journal Title: Journal of Immunology 15;178(2) Pages: 765-70
  • Peer Reviewed
• [Journal Article] A critical role of LEA-1 in the development of Th17 cells and induction of experimental autoimmune encephalomyelitis. (2007)
  • Author(s): Wang Y, et. al.
  • Journal Title: Biochem Biophys Res Commun 23;353 Pages: 857-62
  • Peer Reviewed
• [Presentation] Negative regulation of IgA production by the Fcα/μ recepto, an Fc receptor for IgA and IgM. (2008)
  • Author(s): KURITA Naoki, HONDA Shin-ichiro, CHO Yukiko, USUI Kenta, TAKESHITA Kie, TAHARA-HANAOKA Satoko, SHIBUYA Kazuko and SHIBUYA Akira.
  • Organizer: 第3回JSI-RCAI免疫ワークショップ
  • Place of Presentation: 理化学研究所・横浜研究所
  • Year and Date: 2008-03-14
• [Presentation] Activation of neutrophils by a novel triggering immunoglobulin-1ike receptor MAIR-IV (2008)
  • Author(s): Takako Nakano, Satoko Tahara-Hanaoka, Chigusa Nakahashi-Oda, Ismail Can, Naoya Totsuka, Kaori Hitomi, Satoru Someya, Shin-ichiro Honda, Kazuko Shibuya, and Akira Shibuya.
  • Organizer: Keystone Symposia
  • Place of Presentation: Keystone USA
  • Year and Date: 2008-02-25
• [Presentation] IgM suppresses humoral immune responses against T-independent antigens via the Fcα/μR (2007)
  • Author(s): HONDA Shin-ichiro, CHO Yukiko, USUI Kenta, KURITA Naoki, TAKESHITA Kie^1, TAKAHASHI Satoru, KINOSHITA Taroh, FUJITA Teizo、 TAHARA-HANAOKA Satoko, SHIBUYA Kazuko and SHIBUYA Akira.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] Caspase-independent cell death by MAIR-V(CD300LF), immunoglobulin-like receptor on myeloid cells (2007)
  • Author(s): Ismail Can, Satoko Tahara-Hanaoka, Chigusa Nakahashi, Takako Nakano, Kaori Hitomi, Shin-ichiro Honda, Kazuko Shibuya, Akira Shibuya
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] A critical role of DNAM-1 in tumor immunosurveillance (2007)
  • Author(s): Iguchi Akiko, SHIBUYA Kazuko, KAI Hirayasu, TAHARA-HANAOKA Satoko, HONDA Shinichiro, SHIBUYA Akira.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] Analysis for the variants of Fcα/μR, a novel Fc receptor for IgA and IgM, expressed in the kidney and testis. (2007)
  • Author(s): USUI Kenta, HONDA Shin-ichiro, KURITA Naoki, MIYAMOTO Akitomo, CHO Yukiko, TAHARA-HANAOKA Satoko, SHIBUYA Kazuko, SHIBUYA Akira
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] Negative regulation of IgA production by the Fca/mR, an Fc receptor for IgA and IgM. (2007)
  • Author(s): KURITA Naoki, HONDA Shin-ichiro, CHO Yukiko, USUI Kenta, TAKESHITA Kie, TAHARA Satoko, SHIBUYA Kazuko, SHIBUYA Akira.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] A critical role of LFA-1 in the development of Th17 cells and induction of experimental autoimmune encep halomyelitis. (2007)
  • Author(s): SHIBATA Kai^1, WANG Yinan^1, KAI Hirayasu^1, TAHARA-HANAOKA Satoko^1, HONDA Shin-ichiro^1, SHIBUYA Akira^1, SHIBUYA Kazuko.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] Critical role of DNAM-1(CD226) in the development of Th1 cells. (2007)
  • Author(s): KAI Hirayasy, IGUCHI Akiko, WANG Yinan, YAMASHITA Yumi, YASUI Teruhito, KIKUTANI Hitoshi, TAHARA-HANAOKA Satoko HONDA Shin-ichiro, SHIBUYA Akira, SHIBUYA Kazuko。
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] A novel triggering neutrophil receptor, MAIR-IV, transduces activating signal via FcRγ-independent as well as dependent pathways. (2007)
  • Author(s): Takako Nakano, Satoko Tahara-Hanaoka, Chigus a Nakahashi, Can Ismail, Naoya Totsuka, Kaori Hitomi, Satoru Someya, Shin-ichiro Honda, Kazuko Shibuya, Akira Shibuya.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] ITIM-bearing Immunoglobulin-like receptor, MAIR-I(CD300a) modulates the inflammatory responses in murine sepsis. (2007)
  • Author(s): Chigusa Nakahashi-Oda, Satoko Tahara-Hanaoka, Kaori Hitomi, Takako Nakano, Can Ismail, Naoya Totsuk a, Teruhito Yasui, Hitoshi Kikutani, Shin-ichiro Hond Kazuko Shibuya, Akira Shibuya.
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] Molecular characterization of Fcα/μR, a novel Fc receptor for IgA and IgM. (2007)
  • Author(s): CHO Yukiko, HONDA Shin-ichiro, USUI Kenta, KURITA Naoki, TAKESHITA Kie, TAHARA-HANAOKA Satoko, SHIBUYA Kazuko and SHIBUYA Akira
  • Organizer: 第37回 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] ITIMを持つ免疫受容体MAIR-Vの機能解析 (2007)
  • Author(s): Ismail Can, Satoko Tahara-Hanaoka, Shin-ichiro Honda, Kazuko Shibuya, Akira Shibuya.
  • Organizer: 第69回 日本血液学会
  • Place of Presentation: 神奈川
  • Year and Date: 2007-10-12
• [Presentation] 好中球の活性化を制御する新規ペア型受容体MAIR-IV (2007)
  • Author(s): 中埜 貴子、田原 聡子、中橋 ちぐさ、Ismail Can、戸塚 直也、本多 伸一郎、渋谷 和子、渋谷 彰
  • Organizer: 第69回 日本血液学会
  • Place of Presentation: 神奈川
  • Year and Date: 2007-10-12
• [Presentation] ヘルパーT細胞免疫応答におけるDNAM-1の役割 (2007)
  • Author(s): 甲斐平康、渋谷和子、井口研子、田原聡子、本多伸一郎、渋谷彰
  • Organizer: KTCC
  • Place of Presentation: 京都
  • Year and Date: 2007-06-16
• [Presentation] Control of Germinal Center Reaction against TIndependent Antigens by the Fc(alpha)/(mu)Receptor. (2007)
  • Author(s): Akira Shibuya, Ukiko Cho, Satoko Tahara-Hanaoka, kazuko Shibuiya, Shin-ichiro Honda.
  • Organizer: 94th Annual Meeting The American Association of Immunologists.
  • Place of Presentation: USA
  • Year and Date: 2007-05-21
• [Presentation] Physical and functional association of MAIR-II with ITAM containing adapters FcRγ chain as well as DAP12 in activated macroph (2007)
  • Author(s): A Shibuya, C Nakahashi, N Totsuka, S Tahara-Hanaoka.
  • Organizer: Keystone Symposia
  • Place of Presentation: Colorado, USA
  • Year and Date: 2007-04-14