DNA vaccine targeting both liver and blood stage of virulent strain Plasmodium

2008 Fiscal Year Final Research Report

• Project/Area Number: 19590429
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Kyushu University
• Principal Investigator: HAMANO Shinjiro Kyushu University, 医学研究院, 助教 (70294915)
• Co-Investigator(Kenkyū-buntansha): HIMENO Kunisuke 九州大学, 医学研究院, 教授 (50112339) ; HISAEDA Hajime 九州大学, 医学研究院, 准教授 (50243689) ; HIGO Hiroo 九州大学, 医学研究院, 助教 (80117225)
• Project Period (FY): 2007 – 2008
• Keywords: ユビキチン / プロテアソーム / マラリア / DNAワクチン / CD8^+T細胞 / 遺伝子銃

Research Abstract

マウスマラリアP. yoelii 強毒株 (XL) のスポロゾイト感染系におけるUB-CSP とUB-MSP1によるキメラDNA ワクチンによって、マラリア虫血症および生存率の顕著な延長効果が認められ、P. yoelii XL に対する感染防御能の賦与が明らかとなった。

Research Products

• [Journal Article] Concurrent infection with Heligmosomoides polygyrus modulates murine host response against Plasmodium berghei ANKA infection (2008)
  • Author(s): Tetsutani K, Ishiwata K, Torii M, Hamano S, Hisaeda H, Himeno K
  • Journal Title: Am J Trop Med Hyg 79(6) Pages: 819-22
  • Peer Reviewed
• [Journal Article] Critical contribution of immunoproteasomes in the induction of protective immunity against Trypanosoma cruzi in mice vaccinated with a plasmid encoding a CTL epitope fused to green fluorescence protein (2008)
  • Author(s): Chou B, Hisaeda H, Shen J, Duan X, Imai T, Tu L, Murata S, Tanaka K, Himeno K
  • Journal Title: Microbes Infect 10(3) Pages: 241-50
  • Peer Reviewed
• [Journal Article] Malaria parasites require TLR9 signaling for immune evasion by activating regulatory T cells (2008)
  • Author(s): Hisaeda H, Tetsutani K, Imai T, Moriya C, Tu L, Hamano S, Duan X, Chou B, Ishida H, Aramaki A, Shen J, Ishii KJ, Coban C, Akira S, Takeda K, Yasutomo K, Torii M, Himeno K
  • Journal Title: J Immunol 180(4) Pages: 2496-503
  • Peer Reviewed
• [Journal Article] Antigen-specific CD8^+ T cells induced by the ubiquitin fusion degradation pathway (2008)
  • Author(s): Imai T, Duan X, Hisaeda H, Himeno K
  • Journal Title: Biochem Biophys Res Commun 365(4) Pages: 758-63
  • Peer Reviewed
• [Journal Article] Ubiquitin-fusion degradation pathway : a new strategy for inducing CD8 cells specific for mycobacterial HSP65 (2008)
  • Author(s): Shen J, Hisaeda H, Chou B, Yu Q, Tu L, Himeno K
  • Journal Title: Biochem Biophys Res Commun 365(4) Pages: 621-7
  • Peer Reviewed
• [Journal Article] Immune therapy against tumors based on the ubiquitin-proteasome pathway (2007)
  • Author(s): Himeno K, Hisaeda H
  • Journal Title: Fukuoka Igaku Zasshi(Review. Japanese) 98(8) Pages: 312-9
• [Journal Article] Malaria parasite induces tryptophan-related immune suppression inmice (2007)
  • Author(s): Tetsutani K, To H, Torii M, Hisaeda H, Himeno K
  • Journal Title: Parasitology 134(Pt7) Pages: 923-30
  • Peer Reviewed
• [Journal Article] Identification of Trypanosoma cruzi sublineages by the simple method of single-stranded conformation DNA polymorphism (SSCP) (2007)
  • Author(s): Higo H, Miura S, Agatsuma T, Mimori T, Yanagi T, Iwagami M, de Arias AR, Matta V, Hirayama K, Takeuchi T, Tada I, HimenoK
  • Journal Title: Parasitol Res 100(5) Pages: 1023-31
  • Peer Reviewed
• [Presentation] 消化管寄生虫感染が引き起こす免疫修飾の解析-マラリアとの共感染モデルを用いて (2008)
  • Author(s): 鉄谷耕平
  • Organizer: 第77回日本寄生虫学会大会
  • Place of Presentation: ブリックホール国際会議場、長崎
  • Year and Date: 2008-04-04
• [Presentation] Genetic control of resistance to intestinal amebiasis in inbred mice (2008)
  • Author(s): 濱野真二郎
  • Organizer: 第77回日本寄生虫学会大会
  • Place of Presentation: ブリックホール国際会議場、長崎
  • Year and Date: 2008-04-04
• [Presentation] Activation of regulatory T cells by dendritic cells in mice infected with malaria parasites (2007)
  • Author(s): Hisaeda Hajime
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: グランドプリンスホテル新高輪、東京
  • Year and Date: 2007-11-20
• [Presentation] Involvement of CD8+ T cells in protective immunity against murine malaria blood-stage infection (2007)
  • Author(s): IMAI Takashi
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: グランドプリンスホテル新高輪、東京
  • Year and Date: 2007-11-20
• [Presentation] Application of ubiquitin fusion degradation pathway in DNA vaccination against Trypanosoma cruzi (2007)
  • Author(s): Chou Bin
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: グランドプリンスホテル新高輪、東京
  • Year and Date: 2007-11-20
• [Presentation] マラリア原虫によるToll-likereceptor 9 を介した制御性T 細胞の活性化 (2007)
  • Author(s): 久枝一
  • Organizer: 第76回日本寄生虫学会大会
  • Place of Presentation: 大阪大学
  • Year and Date: 2007-03-29
• [Presentation] Malaria parasite induces tryptophan related immune suppression in mice (2007)
  • Author(s): 鉄谷耕平
  • Organizer: 第76回日本寄生虫学会大会
  • Place of Presentation: 大阪大学
  • Year and Date: 2007-03-29
• [Presentation] マラリアの免疫抑制におけるヘモゾイン関与 (2007)
  • Author(s): 守家慈子
  • Organizer: 第76回日本寄生虫学会大会
  • Place of Presentation: 大阪大学
  • Year and Date: 2007-03-29
• [Presentation] マウスマラリア赤血球ステージ感染制御におけるCD8+T 細胞の関与 (2007)
  • Author(s): 今井孝
  • Organizer: 第76回日本寄生虫学会大会
  • Place of Presentation: 大阪大学
  • Year and Date: 2007-03-29
• [Presentation] トキソプラズマの感染における免疫プロテアソームの関わり (2007)
  • Author(s): 屠麗萍
  • Organizer: 第76回日本寄生虫学会大会
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2007-03-29
• [Presentation] PCRによるTrypanosoma cruziとT. rangeliの鑑別 (2007)
  • Author(s): 肥後廣夫
  • Organizer: 第76回日本寄生虫学会大会
  • Place of Presentation: 大阪大学
  • Year and Date: 2007-03-29
• [Book] 寄生虫による病気in家庭の医学(第6版) (2008)
  • Author(s): 濱野真二郎
  • Total Pages: 1523-1535
  • Publisher: 保健同人社