Molecular characterization of a novel cyanide-sensitive quinol oxidase of Trypanosoma cruzi

2009 Fiscal Year Final Research Report

• Project/Area Number: 19590434
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nagoya City University
• Principal Investigator: SUZUKI Takashi Nagoya City University, 大学院・医学研究科, 研究員 (70305530)
• Research Collaborator: OHSHIMA Shigeru 名古屋市立大学, 大学院・医学研究科, 研究員 ; SUZUKI Mitsuko 名古屋市立大学, 大学院・医学研究科, 研究員 (50507613)
• Project Period (FY): 2007 – 2009
• Keywords: クルーズトリパノソーマ / 酵素 / ミトコンドリア / 増殖

Research Abstract

In this analysis, properties of Trypanosoma cruzi alterative oxidase (TcrAOX) were analyzed. Phylogenetic analysis demonstrated that TcrAOX molecule was placed distantly from other AOX molecules. When TcrAOX was expressed in Escherichia coli cells, the E.coli cells showed increased growth, suggesting an oxidase activity of TcrAOX. In T.cruzi cells, TcrAOX was localized in mitochondria. Over-expression of TcrAOX in T.cruzi cells caused increased growth. Depletion trials of both alleles of TcrAOX failed. Thus TcrAOX was considered to possess an important role for T.cruzi-growth, possibly oxidase activity, while its substrate did not seem to be quinol.

Research Products

• [Journal Article] Purification and kinetic characterization of recombinant alternative oxidase from Trypanosoma brucei brucei. (2010)
  • Author(s): Kido Y, Sakamoto K, Nakamura K, Harada M, Suzuki T, Yabu Y, Saimoto H, Yamakura F, Ohmori D, Moore A, Harada S, Kita K.
  • Journal Title: Biochim Biophys Acta. Epub
  • Peer Reviewed
• [Journal Article] Genomic-scale prioritization of drug targets: the TDR Targets database. (2008)
  • Author(s): Aguero F, Al-Lazikani B, Aslett M, Berriman M, Buckner FS, Campbell RK, Carmona S, Carruthers IM, Chan AW, Chen F, Crowther GJ, Doyle MA, Hertz-Fowler C, Hopkins AL, McAllister G, Nwaka S, Overington JP, Pain A, Paolini GV, Pieper U, Ralph SA, Riechers A, Aguero F, Al-Lazikani B, Aslett M, Berriman M, Buckner FS, Campbell R
  • Journal Title: Nat Rev Drug Discov. 7(11) Pages: 900-7
  • Peer Reviewed
• [Journal Article] Trypanosoma brucei vacuolar protein sorting 41 (VPS41) is required for intracellular iron utilization and maintenance of normal cellular morphology. (2007)
  • Author(s): Lu S, Suzuki T, Iizuka N, Ohshima S, Yabu Y, Suzuki M, Wen L, Ohta N.
  • Journal Title: Parasitology. 134 Pages: 1639-47
  • Peer Reviewed
• [Presentation] Molecular analysis of "movement"-related genes of African trypanosome species. (2009)
  • Author(s): Suzuki T, Ohshima S, Ohashi M, Yabu Y, Miura Y.
  • Organizer: 第78回日本寄生虫学会大会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-27
• [Presentation] (2007)
  • Author(s): Aguero F, Berriman M, Buckner F S, Hertz-Fowler C, Nwaka S, Pain A, Ralph S A, Riecheres A, Roos D S, Shanmugam D, Suzuki T, Verlindre C L, Van Voorhis W C.
  • Organizer: COST B22 Annual Congress
  • Place of Presentation: Dundee, UK.
  • Year and Date: 2007-06-10