2009 Fiscal Year Final Research Report
The investigation on expression and regulation of pathogenic invasive gene in dermatophytes using new transformation system.
| Project/Area Number |
19590457
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Teikyo University |
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Principal Investigator |
MAKIMURA Koichi Teikyo University, 医学部, 准教授 (00266347)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Tsuyoshi 帝京大学, 医真菌研究センター, 講師 (80424331)
NISHIYAMA Yayoi 帝京大学, 医療技術学部, 教授 (10082231)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 病原性 / 白癬菌 / 動物モデル / 形質転換 / 病原因子 / 遺伝子破壊 / 遺伝子診断 |
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| Research Abstract |
Recently the number of patients affected by dermatophytosis are estimated more than 20% in Japan. For investigation on invasive (pathogenic) factor of dermatopytes, development of methods for destruction of target gene of the fungus is essential. In this study we constructed the original methods which are useful for investigation of invasive factor gene in major dermatophytes ; Trichophyton mentagrophytes using animal infectious models. At first we reported the new gene transformation system (Agrobacterium method) using Agrobacterium tumefaciens. Next we construct G418 as the marker of the multiple genes transformation. Finally we developed the Tmku80-destroyed Trichophyton, #49 strain. This strain has destroyed gene Tmku80 which is homolog of ku80 gene of T. mentagrophytes TIMM2789. By making #49 strain, specific gene destruction became possible, and also we could select target gene freely. At the same time we could compare the pathogenesis between modified gene strain and wild type strain of Trichophyton using experimental animal infectious models. Continuously we developed and reported a gene diagnosis system which is useful for human dermatophytosis. The result of this study would contribute to the analysis of clinical condition of dermatophytosis and progress of protection, diagnosis and therapy of the infection.
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[Journal Article] Comparative study of direct PCR, microscopic examination, and culture-based morphological methods for detection and identification of dermatophytes in nail and skin samples2009
Author(s)
Uchida T, Makimura K, Ishihara K, Goto H, Tajiri Y, Okuma M, Fujisaki R, Uchida K, Abe S, Iijima M
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Journal Title
ournal of Dermatology 36(4)
Pages: 202-208
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[Journal Article] Revised culture based identification system for Malassezia Journal of Clinical Microbiology2007
Author(s)
Kaneko T, Makimura K, Abe M, Shiota R, Nakamura Y, Kano R, Hasegawa A, Sugita T, Shibuya S, Watanabe S, Yamaguchi H, Abe S, Okamura N
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Journal Title
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