2009 Fiscal Year Final Research Report
Development of methods of clinical experiment and biomarkers for the assessment of cardiovascular drugs
| Project/Area Number |
19590541
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
UEDA Shinichiro University of the Ryukyus, 大学院・医学研究科, 教授 (80285105)
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| Co-Investigator(Renkei-kenkyūsha) |
OHYA Yusuke 琉球大学, 大学院・医学研究科, 教授 (30240964)
YASU Takanori 琉球大学, 大学院・医学研究科, 准教授 (40265278)
URATA Hidenori 福岡大学, 筑紫病院, 教授 (30289524)
MATSUOKA Hidehiro 久留米大学, 医学部, 准教授 (80248393)
YAMAKAWA Ken 琉球大学, 大学院・医学研究科, 助教 (00363664)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 心血管薬 / 薬効評価システム / サロゲートマーカー |
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| Research Abstract |
1. We assessed methods of the measurement of endothelial function by flow-mediated vasodilatation and of aortic stiffness by pulse wave velocity. We also standardized the method of flow-mediated vasodilatation, which had varied among researchers.
2. We estimated the association of insulin sensitivity measured by glucose clamp technique and serum metabolic marker such as adiponectin.
3. The methods of experiments in humans for the assessment of effects of elevated free fatty acids on angiotensin II forming activity in leukocytes, endothelial function, insulin sensitivity, and leukocyte activation was established. We tested effects of several cardiovascular drugs in this lipid loading experiment mimicking visceral fat obesity. We found that elevated FFA caused endothelial dysfunction, microcirculatory dysfunction (leukocyte activation), insulin resistance partly via leukocyte renin-angiotensin system. This experimental protocol may be useful for the assessment of anti-atherosclerotic drugs. Leukocyte angiotensin II forming activity could be a biomarker for visceral fat obesity linking to vascular dysfunction.
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Research Products
(20 results)
