Development of a novel DNA amplification methods by loop-mediated isothermal amplification in human herpesvirus infections.

2009 Fiscal Year Final Research Report

• Project/Area Number: 19590577
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Fujita Health University (2008-2009); Fujita Health University College (2007)
• Principal Investigator: IHIRA Masaru Fujita Health University, 医療科学部, 准教授 (10290165)
• Co-Investigator(Kenkyū-buntansha): SUGIYAMA Hiroko 藤田保健衛生大学, 医学研究科, 研究員 (10387714) ; ENOMOTO Yoshihiko 藤田保健衛生大学, 医学研究科, 研究員 (00387713) ; YOSHIKAWA Tetsushi 藤田保健衛生大学, 医学部, 准教授 (80288472) ; ASANO Yoshizo 藤田保健衛生大学, 医学部, 教授 (40131180)
• Project Period (FY): 2007 – 2009
• Keywords: 臨床検査医学

Research Abstract

We constructed the rapid diagnostic methods using LAMP were developed for all 8 human herpesvirus infections. Viral DNA was detected in serum without DNA extraction by using LAMP. Furthermore, we developed type specific LAMP methods, which could discriminate between VZV vaccine strain and wild-type strain or between HHV-6 variant A and variant B. Qprobe is expected to become a new method for quantification of viral DNA by the LAMP method.

Research Products

• [Journal Article] Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients. (2010)
  • Author(s): Ihira M, Sugiyama H, Enomoto Y, Higashimoto Y, Sugata K, Asano Y, Yoshikawa T.
  • Journal Title: J Virol Methods. 167(1) Pages: 103-106
  • Peer Reviewed
• [Journal Article] Exanthem subitum-associated encephalitis : nationwide survey in Japan. (2009)
  • Author(s): Yoshikawa T, Ohashi M, Miyake F, Fujita A, Usui C, Sugata K, Suga S, Hashimoto S, Asano Y.
  • Journal Title: Pediatr Neurol. 41(5) Pages: 353-8
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification for discriminating between human herpesvirus 6A and B. (2008)
  • Author(s): Ihira M, Ohta A, Sugata K, Suga S, Asano Y, Yoshikawa T.
  • Journal Title: J Virol Methods. 154(1-2) Pages: 223-225
  • Peer Reviewed
• [Journal Article] Elevated serum cytokine levels are associated with human herpesvirus 6 reactivation in hematopoietic stem cell transplantation recipients. (2008)
  • Author(s): Fujita A, Ihira M, Suzuki R, Enomoto Y, Sugiyama H, Sugata K, Suga S, Asano Y, Yagasaki H, Kojima S, Matsumoto K, Kato K, Yoshikawa T.
  • Journal Title: J. Infect. 57(3) Pages: 241-248
  • Peer Reviewed
• [Journal Article] Discriminating between varicella-zoster virus vaccine and wild-type strains by loop-mediated isothermal amplification. (2008)
  • Author(s): Higashimoto Y, Ihira M, Ohta A, Inoue S, Usui C, Asano Y, Yoshikawa T.
  • Journal Title: J Clin Microbiol 46(8) Pages: 2665-2670
  • Peer Reviewed
• [Journal Article] Human herpesvirus 6 infection in adult living related liver transplant recipients. (2008)
  • Author(s): Ohashi M, Sugata K, Ihira M, Asano Y, Egawa H, Takada Y, Uemoto S, Yoshikawa T.
  • Journal Title: Liver Transpl. 14(1) Pages: 100-109
  • Peer Reviewed
• [Journal Article] Rapid detection of human herpesvirus 8 DNA using loop-mediated isothermal amplification. (2007)
  • Author(s): Kuhara T, Yoshikawa T, Ihira M, Watanabe D, Tamada Y, Katano H, Asano Y, Matsumoto Y.
  • Journal Title: J Virol Methods. 144(1-2) Pages: 79-85
  • Peer Reviewed
• [Journal Article] Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method. (2007)
  • Author(s): Ihira M, Akimoto S, Miyake F, Fujita A, Sugata K, Suga S, Ohashi M, Nishimura N, Ozaki T, Asano Y, Yoshikawa T.
  • Journal Title: J Clin Virol. 39(1) Pages: 22-26
  • Peer Reviewed
• [Presentation] Qprobeを用いた定量的HSV LAMP法の開発 (2010)
  • Author(s): 井平勝
  • Organizer: 第2回LAMP研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-06
• [Presentation] Factors in Association with Three Important Herpesviruses (CMV, HHV-6, and EBV) Infection after Hematopoietic Stem Cell Transplantation (2009)
  • Author(s): Yoshikawa T
  • Organizer: 14^<th> International Conference on Immunobiology and Prophylaxis of Human Herpesvirus Infections
  • Place of Presentation: Japan Kobe
  • Year and Date: 2009-10-06
• [Presentation] Direct detection of EBV DNA in serum by loop mediated isothermal amplification in patients with infectious mononucleosis. (2009)
  • Author(s): Sugiyama H
  • Organizer: 34th Annual International Herpesvirus Workshop
  • Place of Presentation: USA Ithaca
  • Year and Date: 2009-07-29
• [Presentation] Direct detection of HHV-6 DNA in serum by variant specific loop mediated isothermal amplification in hematopoietic stem cell transplant recipients. (2009)
  • Author(s): Ihira M
  • Organizer: 34th Annual International Herpesvirus Workshop
  • Place of Presentation: USA Ithaca
  • Year and Date: 2009-07-27
• [Presentation] Variant特異的HHV-6 LAMP法による造血幹細胞移植後HHV-6感染モニタリング (2009)
  • Author(s): 井平勝
  • Organizer: 第1回LAMP研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-07
• [Presentation] Variant特異的HHV-6 LAMP法による造血幹細胞移植後HHV-6感染モニタリング (2008)
  • Author(s): 井平勝
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-27
• [Presentation] Direct detection of HHV-6 DNA from serum by variant specific Loop mediated isothermal amplification method (LAMP) after hematopoietic stem cell transplantation (HSCT). (2008)
  • Author(s): Ihira M
  • Organizer: 6th International Conference on HHV-6&7
  • Place of Presentation: USA Boltimore
  • Year and Date: 2008-06-20
• [Presentation] Monitoring of human herpesvirus load in peripheral blood of hematopoietic stem cell transplant recipients. (2007)
  • Author(s): Ihira M
  • Organizer: IDSA
  • Place of Presentation: USA San Diego
  • Year and Date: 2007-10-06