2009 Fiscal Year Final Research Report

Toxicokinetics of organophosphorus insecticide-Development and application of the analytical method for the metabolites by CE-MS-

Research Project

Project/Area Number	19590678
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Legal medicine
Research Institution	University of the Ryukyus
Principal Investigator	FUKE Chiaki University of the Ryukyus, 医学部, 准教授 (60173374)
Research Collaborator	NOGUTI Masamiti 琉球大学, 大学院・医学研究科
Project Period (FY)	2007 – 2009
Keywords	キャピラリー電気泳動質量分析法 / 有機リン系農薬 / 代謝物 / 生体試料 / 中毒
Research Abstract	We developed a determination method for the dialkylphosphates (DAPs), metabolites of organophosphate insecticides, using capillary electrophoresis/mass spectrometer (CE/MS) and applied this method to serum and urine collected from poisoned patients with organophosphate insecticide. The 7 DAPs were detected by CE-MS. In all of 29 caces of organophosphate insecticide poisoning, the expected DAPs were detected using this method. O,S-DMTP was also detected. The phosphate type and single or mix formulation of ingested organophosphate insecticide could be distinguished by determination of DAPs. There was a relation between DMP concentration in the serum and the degree of toxic symptoms.