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2010 Fiscal Year Final Research Report

Reconstruction of the benzodiazepine screening using the new technologies of mass spectrometry

Research Project

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Project/Area Number 19590682
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionNippon Medical School

Principal Investigator

HAYASHIDA Makiko  Nippon Medical School, 医学部, 准教授 (60164977)

Co-Investigator(Kenkyū-buntansha) TERADA Masaru  東邦大学, 医学部, 准教授 (30104649)
KUDO Keiko  九州大学, 医学研究科, 講師 (10186405)
KURISAKI Emiko  福島県立医科大学, 医学部, 講師 (30106356)
UEKUSA Kyoko  日本医科大学, 医学部, テクニカルスタッフ (50409215)
YAMAGUCHI Koji  日本医科大学, 医学部, 助教 (90465344)
Project Period (FY) 2007 – 2010
Keywordsベンゾジアゼピン系薬物 / 質量分析 / LC-TOF / LC-MS-MS / GC-MS / スクリーニング / 生体試料 / 固相抽出
Research Abstract

Toxicological analysis with mass chromatography-spectrometry has spread widely and quickly with the progress in ionizing methods. This study was carried out by including new developments in mass chromatography-spectrometry research for forensic toxicology. Time-of-flight (TOF) mass spectrometry (MS) coupled with liquid chromatography (LC) has been developed for screening and for determining benzodiazepines with an exact mass database. All benzodiazepines have similar chemical structures and molecular weights, and, thus, show similar mass spectra and protonated molecule ions. Discrimination of mass spectrometry at low resolving power with LC-MS is usually difficult.
Benzodiazepines are widely used in medical practice in Japan, and data acquired from LC/TOF-MS measurements of 130 drugs, including benzodiazepines, were used to create an exact mass database. This database comprises molecular formulae, calculated exact masses, retention times and calibrations. In the analysis of samples from patients who had taken many drugs, selectivity was improved when the LC/TOF-MS exact mass database was used.
Comprehensive screening with LC/quadrupole TOF is the most effective method in forensic toxicology for analyzing the samples from cases of multidrug overdose. New developmental mass chromatography-spectrometry equipment is required because of the more precise and exact analyses in forensic toxicology.

  • Research Products

    (19 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] 法医中毒学における質量分析研究の新展開2011

    • Author(s)
      林田眞喜子,大野曜吉
    • Journal Title

      日本医科大学医学会雑 7

      Pages: 8-15

    • Peer Reviewed
  • [Journal Article] Fatal human poisoning with Padan^<TM> : a cartap-containing pesticide.2010

    • Author(s)
      Kurisaki E, Kato N, Ishida T, Matsumoto A, Shinohara K, Hiraiwa K
    • Journal Title

      Clin Toxicol 48

      Pages: 153-155

    • Peer Reviewed
  • [Journal Article] 分析ラボ紹介-日本医科大学法医学教室2010

    • Author(s)
      林田真喜子,植草協子,仁平信,大野曜吉
    • Journal Title

      中毒研究 23

      Pages: 68-72

  • [Journal Article] Urinary morphine and codeine concentrations after ingestion of bean-jam buns decorated with poppy seeds.2010

    • Author(s)
      Yamaguchi K, Hayashida M, Hayakawa Y, Nihira M, Ohno Y
    • Journal Title

      Forensic Toxicology 29

      Pages: 69-71

    • Peer Reviewed
  • [Journal Article] Fatal human poisoning with Padan^<TM> : a cartap-containing pesticide.2010

    • Author(s)
      Kurisaki E, Kato N, Ishida T, Matsumoto A, Hiraiwa K
    • Journal Title

      Clinical Toxicology 48

      Pages: 153-155

    • Peer Reviewed
  • [Journal Article] TOF-MS accurate mass database for benzodiazepine screening.2009

    • Author(s)
      Hayashida M, Takino M, Uekusa K, Terada M, Kurisaki E, Kudo K
    • Journal Title

      Legal Med 11

      Pages: S423-S425

    • Peer Reviewed
  • [Journal Article] GC/MS analysis of a herbal dietary supplement containing ephedrine.2009

    • Author(s)
      Uekusa K, Ono T, Hayashida M, Nihira M, Ohno Y
    • Journal Title

      Legal Med 11

      Pages: S573-S575

    • Peer Reviewed
  • [Journal Article] An accidental case of acute poisoning due to Kampo herbal medicine ingestion.2009

    • Author(s)
      Ono T, Hayashida M, Uekusa K, Cui FL, Hayakawa H, Nihira M, Ohno Y
    • Journal Title

      Legal Med 11

      Pages: 132-135

    • Peer Reviewed
  • [Journal Article] Construction of calibration-locking databases for rapid and reliable drug screening by gas chromatography-mass spectrometry.2009

    • Author(s)
      Kudo K, Ishida, T, Hikiji W, Hayashida M, Uekusa K, Usumoto Y, Tsuji A, Ikeda N
    • Journal Title

      Forensic Toxicol 27

      Pages: 21-31

    • Peer Reviewed
  • [Journal Article] Rapid and quantitative screening method for 43 benzodiazepines and their metabolites, zolpidem and zopiclone in human plasma by liquid chromatography/mass spectrometry with a small particle column2009

    • Author(s)
      Ishida T, Kudo K, Hayashida M, Ikeda N
    • Journal Title

      J of Chromatogr B 877

      Pages: 2652-2657

    • Peer Reviewed
  • [Presentation] 剖検試料中セルトラリン,ニトラゼパム,アルプラゾラムのLC/MS分析2009

    • Author(s)
      林田眞喜子,植草協子,工藤恵子,大野曜吉
    • Organizer
      日本法中毒学会第28年会
    • Place of Presentation
      金沢
    • Year and Date
      20090600
  • [Presentation] ベンゾジアゼピン系薬物を含む多剤併用薬物中毒症例へのTOF-MS薬物スクリーニングの応用2009

    • Author(s)
      林田眞喜子,滝埜昌彦,植草協子,工藤恵子,寺田賢,栗崎恵美子,大野曜吉
    • Organizer
      日本法中毒学会第28年会
    • Place of Presentation
      金沢
    • Year and Date
      20090600
  • [Presentation] FOCUSカラムを用いたアコニチン系アルカロイドのLC/MS分析2009

    • Author(s)
      林田眞喜子,植草協子,工藤恵子,石田知己,大野曜吉
    • Organizer
      第93次日本法医学会学術全国集会
    • Place of Presentation
      大阪
    • Year and Date
      20090500
  • [Presentation] Application of Time-of-Flight Mass Spectrometry (TOF-MS) to Multiple-Drug Poisoning Cases.2009

    • Author(s)
      Hayashida M, Takino M, Kudo K, Terada M, Ohno Y
    • Organizer
      The International Association of Forensic Toxicologists 47th International meeting
    • Place of Presentation
      Geneve
    • Year and Date
      20090000
  • [Presentation] TOF-MS accurate mass database for benzodiazepine screening.2008

    • Author(s)
      Hayashida M, Takino K, Uekusa K, Terada M, Kurisaki E, Kudo K
    • Organizer
      7th International Symposium Advances in Legal Medicine
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      20080900
  • [Presentation] エチゾラムとトリアゾラム及び代謝物のLC/MS-MS分析による識別と定量2008

    • Author(s)
      林田眞喜子,植草協子,栗崎恵美子,寺田賢,工藤恵子,滝埜昌彦,他
    • Organizer
      第92次日本法医学会総会
    • Place of Presentation
      長崎県長崎市
    • Year and Date
      20080400
  • [Presentation] UPLCを用いたベンゾジアゼピン系スクリーニング法2007

    • Author(s)
      林田眞喜子,寺田賢,栗崎恵美子,工藤恵子,仁平信,他
    • Organizer
      日本中毒学会総会
    • Place of Presentation
      東京都品川区
    • Year and Date
      20070700
  • [Presentation] LC/TOF-MS及び精密質量データベースを用いたベンゾジアゼピン系薬物のスクリーニング2007

    • Author(s)
      林田眞喜子,滝埜昌彦,寺田賢,仁平信,他
    • Organizer
      日本法中毒学会第26年会
    • Place of Presentation
      宮崎県延岡市
    • Year and Date
      20070600
  • [Remarks] ホームページ等

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Published: 2012-01-26   Modified: 2016-04-21  

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