2009 Fiscal Year Final Research Report
Identification of Genomic Locus Related to Sensitivity Against Anticancer Drug and Predisposition of Pancreatic Cancer Using Comprehensive Analysis
| Project/Area Number |
19590751
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
SASAHIRA Naoki The University of Tokyo, 医学部附属病院, 助教 (30401102)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TADA Minoru 東京大学, 医学部附属病院, 講師 (80302719)
ASAOKA Yoshinari 東京大学, 医学部附属病院, 助教 (90431858)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Keywords | 膵臓病学 / 高密度SNPアレイ |
|---|
| Research Abstract |
We analyzed genome-wide copy number alterations and loss of heterozygosity (LOH) in 25 established pancreatic cancer cell lines using a high-density single nucleotide polymorphism (SNP) array and found eight amplified regions and eight deleted regions in at least 2 cell lines. We then examined representative genes at the eight amplified loci in matched pairs of pancreatic cancer and normal tissues. The amplification was detected in 1 (7.1%) to 5 (35.7%) of 14 microdissected tissue specimens. Then we analyzed the relationship between SNP of cytochrome P450 and the susceptibility of pancreatic cancer. Genetic polymorphism of CYP2C19 was associated with the susceptibility to pancreatic cancer, but it was not significant statistically. In the case of bile duct cancer, it was proved to be significant.
|
