Role of microRNA in embryonic stem cell differentiation into cardiovascular cells

2008 Fiscal Year Final Research Report

• Project/Area Number: 19590827
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TAKAHASHI Tomosaburo Kyoto Prefectural University of Medicine, 医学研究科, 助教 (20381973)
• Project Period (FY): 2007 – 2008
• Keywords: 心臓病態学

Research Abstract

本研究では、最近、新しい細胞機能制御機構として注目されている機能性非翻訳RNA であるmiRNAの胚性幹細胞の心血管細胞分化における役割を検討し、心血管細胞分化に伴い発現が変動する多数のmiRNA を同定し、その内、miR-1が筋細胞分化に促進的に働き、さらにこのmiRNA生成系が細胞分化・血管内皮細胞機能に深く関わっていることを明らかにした。

Research Products

• [Journal Article] Altered expression of Na(+) transporters at the mRNA level in rat normal and hypertrophic myocardium (2009)
  • Author(s): Yamamoto T, Shirayama T, Takahashi T, Matsubara H
  • Journal Title: Heart Vessels 24 Pages: 54-62
• [Journal Article] 【虚血性心疾患 診断と治療の進歩】最近の話題心臓再生医療の現状と将来 (2009)
  • Author(s): 竹原有史, 天野克也, 高橋知三郎, 王英正, 松原弘明
  • Journal Title: 日本内科学会雑誌 98 Pages: 343-350
• [Journal Article] Downregulation of Dicer expression by serum withdrawal sensitizes human endothelial cells to apoptosis (2008)
  • Author(s): Asada S, Takahashi T, Isodono K, Adachi A, Imoto H, Ogata T, Ueyama T, Matsubara H, Oh H.
  • Journal Title: Am J Physiol Heart Circ Physiol 295 Pages: H2512-2521
• [Journal Article] MURC, a muscle-restricted coiled-coil protein that modulates the Rho/ROCK pathway, induces cardiac dysfunction and conduction disturbance (2008)
  • Author(s): Ogata T, Ueyama T, Isodono K, Tagawa M, Takehara N, Kawashima T, Harada K, Takahashi T, Shioi T, Matsubara H, Oh H.
  • Journal Title: Mol Cell Biol 28 Pages: 3424-3436
• [Journal Article] Controlled delivery of basic fibroblast growth factor promotes human cardiosphere-derived cell engraftment to enhance cardiac repair for chronic myocardial infarction (2008)
  • Author(s): Takehara N, Tsutsumi Y, Tateishi K, Ogata T, Tanaka H, Ueyama T, Takahashi T, Takamatsu T, Fukushima M, Komeda M, Yamagishi M, Yaku H, Tabata Y, Matsubara H, Oh H.
  • Journal Title: J Am Coll Cardiol 52 Pages: 1858-1865
• [Journal Article] Crossveinless-2 Controls Bone Morphogenetic Protein Signaling during Early Cardiomyocyte Differentiation in P19 Cells (2008)
  • Author(s): Harada K, Ogai A, Takahashi T, Kitakaze M, Matsubara H, Oh H.
  • Journal Title: J Biol Chem 283 Pages: 26705-26713
• [Journal Article] MURC, a muscle-restricted coiled-coil protein, is involved in the regulation of skeletal myogenesis (2008)
  • Author(s): Tagawa M, Ueyama T, Ogata T, Takehara N, Nakajima N, Isodono K, Asada S, Takahashi T, Matsubara H, Oh H.
  • Journal Title: Am J Physiol Cell Physiol 295 Pages: C490-498
• [Journal Article] Skeletal muscle-derived progenitors capable of differentiating into cardiomyocytes proliferate through myostatin-independent TGF-beta family signaling (2008)
  • Author(s): Nomura T, Ueyama T, Ashihara E, Tateishi K, Asada S, Nakajima N, Isodono K, Takahashi T, Matsubara H, Oh H.
  • Journal Title: Biochem Biophys Res Commun 365 Pages: 863-869
• [Journal Article] Rationale and design of the KYOTO HEART study : effects of valsartan on morbidity and mortality in uncontrolled hypertensive patients with high risk of cardiovascular events (2008)
  • Author(s): Sawada T, Takahashi T, Yamada H, Dahlof B, Matsubara H.
  • Journal Title: J Hum Hypertens
• [Journal Article] 【"難病"診療の最前線】心血管難病に挑む再生医療 (2008)
  • Author(s): 的場聖明, 高橋知三郎, 松原弘明
  • Journal Title: 京都府立医科大学雑誌 117 Pages: 783-792
• [Journal Article] 心臓血管における組織工学ヒト心臓由来多能性幹細胞とbFGF徐放生体吸収ゼラチンゲルシート同時移植による心筋再生医療 (2008)
  • Author(s): 竹原有史, 天野克也, 高橋知三郎, 王英正, 夜久均, 田畑泰彦, 松原弘明
  • Journal Title: 人工臓器 37 Pages: S-32
• [Journal Article] New targeted angiogenic strategy : bursting bubbles (2007)
  • Author(s): Takahashi T, Matsubara H
  • Journal Title: Circ Res 101 Pages: 232-233
• [Journal Article] Stage-specific role of endogenous Smad2 activation in cardiomyogenesis of embryonic stem cells (2007)
  • Author(s): Kitamura R, Takahashi T, Nakajima N, Isodono K, Asada S, Ueno H, Ueyama T, Yoshikawa T, Matsubara H, Oh H.
  • Journal Title: Circ Res 101 Pages: 78-87
• [Journal Article] Central role of calcium-dependent tyrosine kinase PYK2 in endothelial nitric oxide synthase-mediated angiogenic response and vascular function (2007)
  • Author(s): Matsui A, Okigaki M, Amano K, Adachi Y, Jin D, Takai S, Yamashita T, Kawashima S, Kurihara T, Miyazaki M, Tateishi K, Matsunaga S, Katsume A, Honshou S, Takahashi T, Matoba S, Kusaba T, Tatsumi T, Matsubara H.
  • Journal Title: Circulation 116 Pages: 1041-1051
• [Journal Article] Clonally amplified cardiac stem cells are regulated by Sca-1 signaling for efficient cardiovascular regeneration (2007)
  • Author(s): Tateishi K, Ashihara E, Takehara N, Nomura T, Honsho S, Nakagami T, Morikawa S, Takahashi T, Ueyama T, Matsubara H, Oh H.
  • Journal Title: J Cell Sci 120 Pages: 1791-1800
• [Journal Article] Enhanced activity of ventricular Na+-HCO3-cotransport in pressure overload hypertrophy (2007)
  • Author(s): Yamamoto T, Shirayama T, Sakatani T, Takahashi T, Tanaka H, Takamatsu T, Spitzer KW, Matsubara H.
  • Journal Title: Am J Physiol Heart Circ Physiol 293 Pages: H1254-H1264
• [Journal Article] Human cardiac stem cells exhibit mesenchymal features and are maintained through Akt/GSK-3beta signaling (2007)
  • Author(s): Tateishi K, Ashihara E, Honsho S, Takehara N, Nomura T, Takahashi T, Ueyama T, Yamagishi M, Yaku H, Matsubara H, Oh H.
  • Journal Title: Biochem Biophys Res Commun 352 Pages: 635-641
• [Journal Article] Skeletal myosphere-derived progenitor cell transplantation promotes neovascularization in delta-sarcoglycan knockdown cardiomyopathy (2007)
  • Author(s): Nomura T, Ashihara E, Tateishi K, Asada S, Ueyama T, Takahashi T, Matsubara H, Oh H.
  • Journal Title: Biochem Biophys Res Commun 352 Pages: 668-674
• [Journal Article] Osteopontin is a myosphere-derived secretory molecule that promotes angiogenic progenitor cell proliferation through the phosphoinositide 3-kinase/Akt pathway (2007)
  • Author(s): Ogata T, Ueyama T, Nomura T, Asada S, Tagawa M, Nakamura T, Takahashi T, Matsubara H, Oh H.
  • Journal Title: Biochem Biophys Res Commun 359 Pages: 341-347
• [Journal Article] Spironolactone modulates expressions of cardiac mineralocorticoid receptor and 11beta-hydroxysteroid dehydrogenase 2 and prevents ventricular remodeling in post-infarct rat hearts (2007)
  • Author(s): Takeda M, Tatsumi T, Matsunaga S, Hayashi H, Kimata M, Honsho S, Nishikawa S, Mano A, Shiraishi J, Yamada H, Takahashi T, Matoba S, Kobara M, Matsubara H.
  • Journal Title: Hypertens Res 30 Pages: 427-437
• [Journal Article] 【冠動脈疾患をめぐる最近の話題】心筋梗塞への再生医療Update骨髄単核球を利用した細胞治療の現状 (2007)
  • Author(s): 五十殿弘二, 浅田聡, 高橋知三郎, 辰巳哲也, 松原弘明
  • Journal Title: 内科 100 Pages: 529-535
• [Presentation] A novel but evolutionary conserved muscle-restricted coiled-coil protein, MURC, regulates myogenic differentiation in C2C12 cells (2008)
  • Author(s): Tagawa M, Ueyama T, Ogata T, Nakajima N, Itodono K, Asada S, Takehara N, Takahashi T, Matsubara H, Oh H.
  • Organizer: 第72回日本循環器学会総会・学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20080300
• [Presentation] A Novel Muscle-restricted Coiled-coil Protein, MURC, Induces Cardiac Dysfunction Accompanied with an Altered Gene Expression Profile (2008)
  • Author(s): Ogata T, Ueyama T, Isodono K, Tagawa M, Takehara N, Kawashima T, Shioi T, Takahashi T, Harada K, Matsubara H, Oh H.
  • Organizer: 第72回日本循環器学会総会・学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20080300
• [Presentation] Cellular functions of Dicer in human endothelial cells (2008)
  • Author(s): Asada S, Takahashi T, Isodono K, Imoto H, Adachi A, Ueyama T, Matsubara H, Oh H.
  • Organizer: 第72回日本循環器学会総会・学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20080300
• [Presentation] Crossveinless-2 antagonizes the bone morphogenetic protein signaling to regulate cardiac mesodermal specification and differentiation in P19Cl6 embryonic carcinoma cells (2008)
  • Author(s): Harada K, Takahashi T, Matsubara H, Oh H.
  • Organizer: 第72回日本循環器学会総会・学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20080300
• [Presentation] Dose-dependent Functional Recovery of Human Cardiac Stem Cell Transplantation Combined with Control-Delivery of Angiogenic Growth Factor in Chronic Myocardial Infarction (2008)
  • Author(s): Takehara N, Tsutsumi Y, Tateishi K, Ogata T, Ueyama T, Takahashi T, Tanaka H, Komeda M, Yamagishi M, Yaku H, Tabata Y, Matsubara H, Oh H.
  • Organizer: 第72回日本循環器学会総会・学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20080300
• [Presentation] PARM-1, a Novel Transmembrane Molecule Involved in Endoplasmic Reticulum Stress-Induced Apoptosis in Cardiac Myocytes (2008)
  • Author(s): Isodono K, Takahashi T, Ogata T, Asada S, Imoto H, Adachi A, Ueyama T, Matsubara H, Oh H.
  • Organizer: 第72回日本循環器学会総会・学術集会
  • Place of Presentation: 福岡
  • Year and Date: 20080300
• [Presentation] 心筋・骨格筋特異的microRNA-1は、骨格筋細胞分化を制御する (2007)
  • Author(s): 中島規雄, 高橋知三郎, 北村亮治, 五十殿弘二, 浅田聡, 上山知己, 松原弘明, 王英正
  • Organizer: 第36回日本心脈管作動物質学会
  • Place of Presentation: 徳島
  • Year and Date: 20070000
• [Presentation] 胚性幹細胞における内因性Smad2活性化による心筋細胞分化の時期特異的な相反する制御 (2007)
  • Author(s): 北村亮治, 高橋知三郎, 中島規雄, 浅田聡, 五十殿弘二, 上山知己, 松原弘明, 王英正
  • Organizer: 第36回日本心脈管作動物質学会
  • Place of Presentation: 徳島
  • Year and Date: 20070000
• [Presentation] やさしい再生医療-幹細胞と心筋再生- (2007)
  • Author(s): 高橋知三郎, 王英正, 松原弘明
  • Organizer: 第71回日本循環器学会総会・学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20070000
• [Presentation] MicroRNA-1 Facilitates Skeletal Myogenic Differentiation without Affecting Osteoblastic and Adipogenic Differentiation (2007)
  • Author(s): Nakajima N, Takahashi T, Kitamura R, Isodono K, Asada S, Ueyama T, Matsubara H, Oh H.
  • Organizer: 第71回日本循環器学会総会・学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20070000
• [Presentation] Endogenous TGF-β Negatively Regulates Cardiac Differentiation through Smad2 Activation in Embryonic Stem Cells (2007)
  • Author(s): Kitamura R, Takahashi T, Nakajima N, Asada S, Isodono K, Ueno H, Ueyama T, Matsubara H, Oh H.
  • Organizer: 第71回日本循環器学会総会・学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20070000
• [Presentation] Myostatin-independent Activin Type II Receptor Ligands Signaling Regulate the Growth of Postnatal Muscle-derived Cardiac Progenitor Cells (2007)
  • Author(s): Nomura T, Ueyama T, Tateishi K, Asada S, Takahashi T, Matsubara H, Oh H.
  • Organizer: 第71回日本循環器学会総会・学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20070000