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2009 Fiscal Year Final Research Report

Making conditional knockout mouse of the gene coding inversin and the significance of inversion in the cilia-dependent renal disease

Research Project

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Project/Area Number 19590965
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionTokyo Women's Medical University

Principal Investigator

TSUCHIYA Ken  Tokyo Women's Medical University, 医学部, 准教授 (00246472)

Co-Investigator(Renkei-kenkyūsha) YOSHIDA Takumi  東京女子医科大学, 医学部, 助教 (80297449)
TSUCHIYA Mariko  東京女子医科大学, 医学部, 准教授 (00266826)
Project Period (FY) 2007 – 2009
KeywordssiRNA / 左右軸 / ノックアウトマウス / 繊毛 / 嚢胞腎 / inv遺伝子
Research Abstract

Vertebrate organisms have a common left-right asymmetry of their visceral organs. However, all unpaired organs of the chest and abdomen, such as heart, stomach, spleen and liver, develop from the midline in the fetus and localize to their normal positions in the adult. The phenotype of the inv mouse is a consistent mirror-image reversal of the left-right polarity (situs inversus) and cystic formation of the kidneys. In this study, the targeting vector of the inversion of embryonic turning (inv) for conditional knockout mounting interferon-induced promoter was constructed. Genomic southern hybridization indicated that these gene spans the whole deleted region, implying that the homozygous inv mice have intragenic deletion of this gene. We also analyzed the physiologic and pathophysiological findings of mice and renal tubular cells kidney associated with an inversion of embryonic turning. The results suggested that there might be a possibility that a cytoskeletal abnormality was involved in the mechanism and production of both structural abnormalities, inversion and cyst formation in the kidney.

  • Research Products

    (7 results)

All 2010 2009 2008 2007

All Journal Article (7 results) (of which Peer Reviewed: 7 results)

  • [Journal Article] Klotho reduces apoptosis in experimental ischaemic acute kidney injury via HSP-70.2010

    • Author(s)
      Sugiura H, Yoshida T, Mitobe M, Yoshida S, Shiohira S, Nitta K, Tsuchiya K
    • Journal Title

      Nephrol Dial Transplant 25

      Pages: 60-68

    • Peer Reviewed
  • [Journal Article] Suppression of mafA mRNA with siRNA prevents adipose cell differentiation in 3T3-L1 cells.2009

    • Author(s)
      Tsuchiya M, Maeda A, Suzuki A, Yasuda K, Yoshida T, Nitta K, Tsuchiya K*
    • Journal Title

      Int J Mol Med 23

      Pages: 725-732

    • Peer Reviewed
  • [Journal Article] Activating transcription factor 3 ameliorates oxidative stress-induced cell death in human kidney cells and in experimental renal ischemia reperfusion injury.2008

    • Author(s)
      Yoshida T, Sugiura H, Mitobe M, Nishimura S, Shirota S, Tsuchiya K, Nobori K, Nitta K, Akiba T
    • Journal Title

      J Am Soc Nephrol 19

      Pages: 217-214

    • Peer Reviewed
  • [Journal Article] The effect of eicosapentaenoic acid on renal function and volume in patients with ADPKD.2008

    • Author(s)
      Higashihara E, Nutahara K, Horie S, Muto S, Hosoya T, Hanaoka K, Tsuchiya K, (12人中7番目)
    • Journal Title

      Nephrol Dial Transplant 23

      Pages: 2847-2852

    • Peer Reviewed
  • [Journal Article] Increase of integrin-linked kinase activity in cultured podycytes upon stimulation with plasma from patients with recurrent FSGS.2008

    • Author(s)
      Hattori M, Akioka Y, Chikamoto H, Kobayashi N, Tsuchiya K, (8人中5番目)
    • Journal Title

      Am J Transplant 8

      Pages: 1550-1556

    • Peer Reviewed
  • [Journal Article] In vivo suppression of mafA mRNA with siRNA and analysis of the resulting alteration of the gene expression profile in mouse pancreas by the microarray method.2007

    • Author(s)
      Tsuchiya M, Yoshida T, Taniguchi S, Yasuda K, Maeda A, Hayashi A, Tanaka J, Shigemoto M, Nitta K, Tsuchiya K*
    • Journal Title

      Biochem Biophys Res Commun 356

      Pages: 29-135

    • Peer Reviewed
  • [Journal Article] Protective effect of carbon monoxide donor compounds in endotoxin-induced acute renal failure.2007

    • Author(s)
      Shiohira S, Yoshida T, Sugiura H, Shirota S, Tsuchiya K, Akiba T, Nitta K
    • Journal Title

      Am J Nephrol 27

      Pages: 441-446

    • Peer Reviewed

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Published: 2011-06-18   Modified: 2016-04-21  

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