Regeneration research of peripheral nerve injury via macrophage stimulated by oxidized galectin-1

2009 Fiscal Year Final Research Report

• Project/Area Number: 19591004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Nihon institute of Medical Science
• Principal Investigator: YOSHIMURA Kazunori Nihon institute of Medical Science, 保健医療学部, 教授 (20158497)
• Co-Investigator(Renkei-kenkyūsha): KAMETANI Fuyuki (財)東京都医学研究機構, 東京都精神医学総合研究所, 主任研究員 (70186013)
• Research Collaborator: KADOYA Toshihiko 前橋工科大学, 工学部・生物工学科, 教授 (40551875) ; SAKAMOTO Yasushi 埼玉医科大学, 医学部・中央研究施設, 施設長・教授 (80178582) ; KITAMURA Kunio 埼玉医科大学, 保健医療学部・健康医療科学科, 教授 (70049857) ; MIYAZAKI Takashi 埼玉医科大学, 医学部・地域医学医療センター, 助教 (30265417)
• Project Period (FY): 2007 – 2009
• Keywords: 神経病態生化学 / 神経再生促進

Research Abstract

We identified several kind of proteins related with signal transduction of oxidized galectin-1 in macrophages stimulated with oxidized galectin-1 by fluorescence two dimensional difference gel electrophoreis (2D DIGE). The proteins were arginase, GRP78, transaldolase, glia maturation factor, and malate dehydrogenase. We have been investigating about macrophages stimulated by oxidized galecin-1 in detail with Real Time RT-PCR, Western blot, Calcium Imaging, and proteome analysis.

Research Products

• [Journal Article] Biological significance of protein modification in aging and calorie restriction. (2010)
  • Author(s): Nakamura A, Kawamura K, Kametani F, Nakamoto H, Goto S.
  • Journal Title: Ann New York Acad Sci (in press)
  • Peer Reviewed
• [Journal Article] Aberrant expressions of aquaporin-1 in association with capillarized sinusoidal endotherial cells in cirrhotic rat live. (2010)
  • Author(s): Yokomori H., Oda M., Yoshimura K., Watanabe S., Hibi T.
  • Journal Title: Med Mol Morphol. 43(1) Pages: 6-12
  • Peer Reviewed
• [Journal Article] Amyloid fibrils formed by selective N-, C-terminal sequences of mouse apolipoprotein A-II. (2010)
  • Author(s): Sawashita J, Kametani F, Hasegawa K, Tsutsumi-Yasuhara S, Zhang B, Yan J, Mori M, Naiki H, Higuchi, K.
  • Journal Title: Biochim Biophys Acta 1794 Pages: 1517-1529
  • Peer Reviewed
• [Journal Article] A possible connection between psychosomatic symptoms and daily rhythmicity in growth hormone secretion in healthy Japanese students. (2009)
  • Author(s): Nagane, M., Yoshimura, K., Watanabe, SI., Nomura, M.
  • Journal Title: J Circadian Rhytms 7 Pages: 10
  • Peer Reviewed
• [Journal Article] Caveolin-1 and Rac regulate endothelial capillary-like tubular formation and fenestral constraction in sinusoidal endothelial cells. (2009)
  • Author(s): Yokomori, H., Oda, M., Yoshimura, K., Nagai, T., Fujimaki, K., Watanabe, S., Hibi, T.
  • Journal Title: Liver Int 29(2) Pages: 266-276
  • Peer Reviewed
• [Journal Article] A new monoclonal antibody, A3B10, specific for astrocyte-lineage cells recognizes calmodulin-regulated spectrin-associated protein 1. (2009)
  • Author(s): Yamamoto, K., Yoshimura, K., Kitada, K., Nakahara, J., Seiwa, C., Ueki, T., Shimoda, Y., Ishige, A., Watanabe, K., Asou, H.
  • Journal Title: J Neurosci Res 87(2) Pages: 503-513
  • Peer Reviewed
• [Journal Article] Hereditary rimmed vacuole myopathy showing interstitial amyloid deposition in muscle tissue. (2009)
  • Author(s): Kojima Y, Sakai K, Ishida C, Asaka T, Hamaguchi T, Nozaki I, Fukushima K, Tsuchiya A, Kametani F, Yazaki M, Okino S, Yamada M.
  • Journal Title: Muscle & Nerve 40 Pages: 472-475
  • Peer Reviewed
• [Journal Article] Truncation and pathogenic mutations facilitate the formation of intracellular aggregates of TDP-43. (2009)
  • Author(s): Nonaka T, Kametani F, Arai T, Akiyama H, Hasegawa M.
  • Journal Title: Hum Mol Genet 18 Pages: 3353-3364
  • Peer Reviewed
• [Journal Article] Identification of casein kinase-1 phosphorylation sites on TDP-43. (2009)
  • Author(s): Kametani F, Nonaka T, Suzuki T, Arai T, Dohmae N, Akiyama H, Hasegawa M.
  • Journal Title: Biochem Biophys Res Commun 382 Pages: 405-409
  • Peer Reviewed
• [Journal Article] Methylene blue and dimebon inhibit aggregation of TDP-43 in cellular models. (2009)
  • Author(s): Yamashita M, Nonaka T, Arai T, Kametani F, Buchman VL, Ninkina N, Bachurin SO, Akiyama H, Goedert M, Hasegawa M.
  • Journal Title: FEBS Lett 583 Pages: 2419-2424
  • Peer Reviewed
• [Presentation] Proteomic analysis of macrophages stimulated with oxidized galectin-1. (2007)
  • Author(s): Kazunori Yoshimura, Fuyuki Kametani, Kayo Fujimaki, Takashi Miyazaki, Hidenori Horie, Shu-Ichi Watanabe, Toshihiko Kadoya
  • Organizer: 47th Annual Meeting of The American Society for Cell Biology. Annual Meeting Program. No. 2015
  • Place of Presentation: Washington
  • Year and Date: 20071201-20070105
• [Presentation] Proteomic analysis of human astrocytes treated with propofol. (2007)
  • Author(s): Toshiko Maruo, Kazunori Yoshimura, Kayo Fujimaki, Masahio Nomura, Shu-Ichi Watanabe, Masahiko Suzuki, Hideki Miyao
  • Organizer: 2007 Annual Meeting for Experimental Biology. Abstracts, Abstract No. 791.
  • Place of Presentation: Washington Convention Center, 801 Mount Vernon Place, NW, Washington, DC
  • Year and Date: 2007-04-30
• [Presentation] Sho Matsushita. Cerebral metabolism of glucose in chronic intermittent hyoxia: a nuclear magnetic resonance spectroscopy study.
  • Author(s): Masami Nishina, Masahiko Suzuki, Naomi Takehara, M Matsushita, Kazunori Yoshimura
  • Organizer: Program/Abstract#482. 5/RR38. Society for Neuroscience 2008 Meeting.
  • Place of Presentation: Washington Convention Center, C. Washington Convention Center, 801 Mount Vernon Place, NW, Washington, DC
• [Presentation] Analysis of the topology of the microsomal epoxide hydrolase on the cell surface with monoclonal antibodies with different epitope specificiteis.
  • Author(s): Hongying Duan, Kazunori Yoshimura, Nobuharu Kobayashi, Akira Takagi, Masanori Matsui, Satoshi Ohno, Kazuo Sugiyama, Christophe Morisseau, Bruce D. Hammock, Toshitaka Akatsuka
  • Organizer: Program/Abstract #479. 31. Experimental Biology 2008 Meeting.
  • Place of Presentation: San Diego Convention Center, CA, U.S.A.