• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2007 Fiscal Year Annual Research Report

原因遺伝子と酸化ストレスから見た肺高血圧の分子基盤:遺伝子改変マウスを用いた検討

Research Project

Project/Area Number 19591202
Research InstitutionMie University

Principal Investigator

三谷 義英  Mie University, 医学部附属病院, 講師 (60273380)

Co-Investigator(Kenkyū-buntansha) 新保 秀人  三重大学, 大学院・医学系研究科, 教授 (70179076)
丸山 一男  三重大学, 大学院・医学系研究科, 教授 (20181828)
澤田 博文  三重大学, 医学部附属病院, 診療等従事者 (30362354)
Keywords肺高血圧 / 酸化ストレス / 遺伝子異常 / 骨形成蛋白 / 転写因子
Research Abstract

1動物モデルの作成:BMPR2(+/-)マウス,Nrf2(-/-)マウス及びダブルノックアウトマウスに慢性低酸素暴露を行い動物モデルの作成を行った。BMPR2(+/-)の低酸素暴露肺高血圧は増悪せず,Nrf2(-/-)マウスは肺高血圧が増悪した。ダブルノックアウトマウスでは,Nrf2(-/-)の低酸素暴露肺高血圧がさらに増悪した。さらに,右室肥大,組織学的評価よる肺血管病変も増悪した。
2Western blot,RT-PCR,免疫染色による解析:各種酸化ストレス関連蛋白は,低酸素暴露Nrf2(-/-)マウスで亢進し,さらにダブルノックアウトで亢進した。また,prepro-ET1産生,PDE5,はさらに亢進し,eNOS発現は低下した。3ゲルシフトアッセイ:NF-kB-DNA結合は,肺高血圧,血管病変と一致して,より増悪を認めた。

  • Research Products

    (12 results)

All 2008 2007

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (5 results)

  • [Journal Article] Suture repair of pectus excavatum at the time of cardiac surgery on an infant2008

    • Author(s)
      Takabayashi S, Mitani Y, et. al.
    • Journal Title

      Ann Thorac Surg 85

      Pages: 651-653

    • Peer Reviewed
  • [Journal Article] Fate of the‘opened'arterial duct:Lessons learned from bilateral pulmonary artery banding for hypoplastic left heart syndrome under the continuous infusion of prostaglandin E12007

    • Author(s)
      Mitani Y, Ohashi H, et. al.
    • Journal Title

      J Thorac Cardiovasc Surg 133

      Pages: 1653-1654

    • Peer Reviewed
  • [Journal Article] A nuclear factor-κB inhibitor pyrrolidine dithiocarbamate ameriolates pulmonary hypertension in rats.2007

    • Author(s)
      Sawada H, Mitani Y, et. al.
    • Journal Title

      Chest 133

      Pages: 1265-1274

    • Peer Reviewed
  • [Journal Article] A Novel Inhibitor of Inducible Nitric Oxide Synthase,ONO-1714,Does Not Amehorate Hypoxia-induced Puhnoary Hypertension in Rats.2007

    • Author(s)
      Jiang BH, Mitani Y, et. al.
    • Journal Title

      Lung 185

      Pages: 303-308

    • Peer Reviewed
  • [Journal Article] Palliative management of a low-birth-weight infant with congenitally corrected transposition of the great arteries,severe restrictive foramen ovale,hypoplasia of the morphologically right ventricle,ventricular septaldefect,and stenoinsufficiency of the tricuspid valve2007

    • Author(s)
      Takabayashi S, Mitani Y, et. al.
    • Journal Title

      Gen Thorac Cardiovasc Surg 55

      Pages: 163-166

    • Peer Reviewed
  • [Journal Article] Reversal of increased pulmonary arterial pressure associated with systemic venous collaterals after tonsillectomy in a Fontan candidate after Glenn procedure:Impact of obstructive sleep apnea on Fontan circulation.2007

    • Author(s)
      Sawada H, Mitani Y, et. al.
    • Journal Title

      J Thorac Cardiovasc Surg 133

      Pages: 1371-1373

    • Peer Reviewed
  • [Journal Article] Reduced regional right ventricular wall motion after transventricular repair of tetralogy of Fallot.2007

    • Author(s)
      Takabayashi S, Mitani Y, et. al.
    • Journal Title

      J Thorac Cardiovasc Surg 133

      Pages: 1656-1658

    • Peer Reviewed
  • [Presentation] In Vivo Plaque Composition and Morphology in Coronary Artery Lesions in the Adolescents and Young Adults Long after Kawasaki Disease:Lessons Learned from Virtual Histology-lntravascular Ultrasound Study2007

    • Author(s)
      Mitani Y, Ohashi H, et. al.
    • Organizer
      American Heart Association
    • Place of Presentation
      Orland, US
    • Year and Date
      2007-11-05
  • [Presentation] 先天性心疾患の治療計画におけるSildenafilの有用性(第1報):non-PH群への応用2007

    • Author(s)
      三谷 義英, 他
    • Organizer
      日本小児循環器学会総会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-05
  • [Presentation] 骨髄由来細胞と造血幹細胞のマウス実験的肺高血圧への関与2007

    • Author(s)
      加藤 太一、三谷 義英, 他
    • Organizer
      日本小児循環器学会総会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-04
  • [Presentation] Glenn術後のFontan calldidateに合併した肺静脈閉塞の原因と予後2007

    • Author(s)
      大橋 啓之、三谷 義英, 他
    • Organizer
      日本小児循環器学会総会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-04
  • [Presentation] Role of Bosentan in the treatment of pulmonary vascular diseases associated with congenital heart disease2007

    • Author(s)
      Mitani Y
    • Organizer
      The 4th Scientific symposium for pulmonary hypertension
    • Place of Presentation
      Kyoto
    • Year and Date
      2007-06-10

URL: 

Published: 2010-02-04   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi