Aging of human hematopoietic stem cells - Establishment of aging evaluation method of hematopoietic stem cell-

2008 Fiscal Year Final Research Report

• Project/Area Number: 19591257
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kagoshima University
• Principal Investigator: KAWANO Yoshifumi Kagoshima University, 大学院医歯学総合研究科, 教授 (20260680)
• Co-Investigator(Renkei-kenkyūsha): OKAMOTO Yasuhiro 鹿児島大学, 医学部・歯学部附属病院, 講師 (30398002) ; HIRAO Atsushi 金沢大学, がん研究所, 教授 (90343350)
• Project Period (FY): 2007 – 2008
• Keywords: ヒト造血幹細胞 / 造血細胞移植術 / 細胞加齢 / 活性酸素種 / 加齢マーカー

Research Abstract

ヒト造血幹細胞のテロメア長は経年的な漸減傾向を示さないため加齢の指標に利用しにくいこと、ヒト造血幹細胞内に蓄積される活性酸素種は実際の造血幹細胞移植によって短期的には変動しないことを確認した。マウス造血幹細胞で証明されている加齢(老化)マーカーはヒト造血幹細胞では指標にならないことが判明した。

Research Products

• [Journal Article] Severe Neurotoxicities in a Case of Charcot-Marie-Tooth Disease Type 2 Caused by Vincristine for Acute Lymphoblastic Leukemia (2008)
  • Author(s): Nishikawa Takuro, Kawakami, Kiyoshi, Kumamoto, Takashi, Tonooka, Shiro, Abe Akiko, Hayasaka, Kiyoshi, Okamoto Yasuhiro, Kawano Yoshifumi
  • Journal Title: Journal of Pediatric Hematology/Oncology 30 Pages: 519-521
• [Journal Article] Identification of stem cells during prepubertal spermatogenesis via monitoring of nucleostemin promoter activity (2008)
  • Author(s): Ohmura M, Naka K, Hoshii T, Muraguchi T, Shugo H, Tamase A, Uema N, Ooshio T, Arai F, Takubo K, Nagamatsu G, Hamaguchi I, Takagi M, Ishihara M, Sakurada K, Miyaji H, Suda T, Hirao A.
  • Journal Title: Stem Cells 26 Pages: 3237-3246
• [Journal Article] Bone marrow transplantation in children with severe Aplastic anemia using a conditioning regimen containing 3 Gy of total body irradiation, cyclophosphamide with or without antilymphocyte globulin (2007)
  • Author(s): Inagaki J, Nagatoshi Y, Kawano Y, Saito Y, Takahashi D, Nagayama J, Shinkoda Y, Hirata H, Okamura J
  • Journal Title: Pediatr Transplantation 11 Pages: 180-186
• [Presentation] Reactive Oxygen Species in Human Bone Marrow CD34-positive Cells at Day 30 post-Bone Marrow Transplantation were not Increased Compared to those in Grafts at Donation (2008)
  • Author(s): Yuichi Kodama, Yasuhiro Okamoto, Takuro Nishikawa, Yuichi Shinkoda, Takayuki Tanabe, Yoshifumi Kawano
  • Organizer: Keystone Symposia
  • Place of Presentation: Stemcells, cancer, and aging, Singapore
  • Year and Date: 20081004-1129