Development of a novel one-step procedure for articular cartilage repair using minced cartilage and mesenchymal stem

2009 Fiscal Year Final Research Report

• Project/Area Number: 19591750
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Chiba University
• Principal Investigator: NAKAGAWA Koichi Chiba University, 大学院・医学研究院, 講師 (30400823)
• Project Period (FY): 2007 – 2009
• Keywords: 関節病学

Research Abstract

[Objective] To develop a novel implantation procedure without in vitro culture, the effect of co-implantation of minced cartilage and synovial cells on the chondrogenic differentiation of the synovial tissue-derived mesenchymal stem cells was investigated.
[Methods] Rabbit synovial cells were isolated by enzyme treatment for 1h and embedded in fibrin gel. The fibrin gel implants containing only synovial cells (SC group), both minced articular cartilage and synovial cells (AC+SC group) or neither cartilage nor synovial cells (NC group) were implanted subcutaneously in nude mice and then examined the histological and biochemical findings.
[Results] Safranin-O staining tended to be stronger, and proteoglycan content (per weight) was significantly increased in the AC+SC group compared to the SC group. Furthermore, type II collagen and Sox9 expression were significantly enhanced in the AC+SC group.
[Conclusion] Co-implantation of minced cartilage with synovial cells enhances chondrogenic differentiation. The use of implant grafts containing both minced cartilage and synovial cells may be a novel approach for one-step joint cartilage repair.

Research Products

• [Journal Article] Detection of pain-related molecules in the subchondral bone of osteoarthritic knees (2009)
  • Author(s): Ogino S, Sasho T, Nakagawa K, Suzuki M, Yamaguchi M, Higashi M, Takahashi K, Moriya H
  • Journal Title: Clin Rheumatol 29 Pages: 1395-1402
  • Peer Reviewed
• [Journal Article] 細切軟骨片との共存移植が滑膜細胞の軟骨分化に与える影響 (2009)
  • Author(s): Louay Fallouh, 中川晃一, 佐粧孝久, 守屋拓朗, 鶴岡弘章, 東山礼治, 和田佑一, 守屋秀繁, 高橋和久
  • Journal Title: 千葉スポーツ医学研究会雑誌 6 Pages: 1-4
  • Peer Reviewed
• [Journal Article] RGD peptide-induced cell death of chondrocytes and synovial cells (2008)
  • Author(s): Matsuki K, Sasho T, Nakagawa K, Tahara M, Sugioka K, Ochiai N, Ogino S, Wada Y, Moriya H
  • Journal Title: J Orthop Sci 13 Pages: 524-532
  • Peer Reviewed
• [Journal Article] In vivo maturation of scaffold-free engineered articular cartilage on porous hydroxyapatite (2008)
  • Author(s): Kitahara S, Nakagawa K, Sah RL, Wada Y, Ogawa T, Moriya H, Masuda K
  • Journal Title: Tissue Eng Part A 14 Pages: 1905-1913
  • Peer Reviewed
• [Presentation] 細切軟骨片との共存移植が滑膜細胞の軟骨分化に与える影響 (2009)
  • Author(s): Louay Fallouh, 中川晃一, 佐粧孝久, 鶴岡弘章, 和田佑一, 守屋秀繁, 高橋和久
  • Organizer: 第24回日本整形外科学会基礎学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20091105-20091107
• [Presentation] Effect of platelet-rich plasma gel on rabbit articular chondrocytes cultured in alginate beads (2009)
  • Author(s): Fallouh L, Nakagawa K, Sasho T, Arai M, Kitahara S, Wada Y, Moriya H, Takahashi K
  • Organizer: The 8^<th> World Congress of the International Cartilage Repair Society
  • Place of Presentation: Miami, USA
  • Year and Date: 20090523-20090526
• [Presentation] Transplantation of newly developed composite graft using scaffold-free engineered cartilage and porous hydroxyapatite to treat osteochondral defects in a rabbit model (2009)
  • Author(s): Kitahara S, Nakagawa K, Wada Y, Nakajima T, Takahashi K, Moriya H, Sah RL, Masuda K
  • Organizer: The 55^<th> Annual Meeting of the Orthopaedic Research Society (ORS)
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 20090222-20090225
• [Presentation] 細切軟骨片との共存移植による滑膜細胞の軟骨分化促進効果 (2009)
  • Author(s): 中川晃一, Louay Fallouh, 佐粧孝久, 鶴岡弘章, 和田佑一, 守屋秀繁, 高橋和久
  • Organizer: 第28回日本運動器移植・再生医学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-21
• [Presentation] 骨軟骨欠損治療のための新しい移植用軟骨-人工骨複合体の開発 (2008)
  • Author(s): 北原聡太, 中川晃一, 佐粧孝久, 荒井桃子, 和田佑一, 守屋秀繁, 小川哲朗, 中島武彦, 舛田浩一, Robert L. Sah, 高橋和久
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 20081023-20081024
• [Presentation] 関節軟骨細胞との共培養が滑膜細胞の軟骨分化に与える影響の解析 (2008)
  • Author(s): 守屋拓朗, 佐粧孝久, 中川晃一, 北原聡太, 鶴岡弘章, 荒井桃子, 高橋和久, 和田佑一, 山下剛司, 守屋秀繁
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 20081023-20081024
• [Patent(Industrial Property Rights)] 滑膜細胞および細切軟骨片の軟骨修復における利用 (2009)
  • Inventor(s): 中川晃一, 守屋拓郎, 佐粧孝久, 和田佑一
  • Industrial Property Rights Holder: 千葉大学
  • Industrial Property Number: 特許特願2009-040841
  • Filing Date: 2009-02-24