Cellular and molecular biological analysis of hypoxia-inducible genetic responses in lung epithelial cells

2008 Fiscal Year Final Research Report

• Project/Area Number: 19591827
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Tazuke Kofukai Medical Research Institute
• Principal Investigator: ADACHI Takehiko Tazuke Kofukai Medical Research Institute, 第9研究部, 部長 (90252428)
• Co-Investigator(Kenkyū-buntansha): HIROTA Kiichi 京都大学, 大学院医学研究科, 講師 (00283606)
• Co-Investigator(Renkei-kenkyūsha): MIYAZAKI Yoshiya 財団法人田附興風会・医学研究所, 第9研究部, 研究員 (10324625)
• Project Period (FY): 2007 – 2008
• Keywords: 周術期管理学

Research Abstract

肺障害の成因または進展を細胞の低酸素誘導性遺伝子応答の観点から理解して肺障害治療法の評価を新たな観点から下し、また新たな治療法の開発の基礎的な知見を得ることが本研究の目的であった。
培養細胞株、マウスを用いた検討により肺胞水分量の調節に重要な働きをしているamiloride-blockable ENaC (SCNN1)の3種類のサブユニットと水チャネル(aquaporin)の酸素分圧依存性の発現変化を観察してhypoxia-inducible factor 1の活性化との相関に関する知見を得た。

Research Products

• [Journal Article] The calcium channel blocker cilnidipine selectively suppresses hypoxia-inducible factor 1 activity in vascular cells (2009)
  • Author(s): Oda S, Oda T, Takabuchi S, Nishi K, Wakamatsu T, Tanaka T, Adachi T, Fukuda K, Nohara R, Hirota K
  • Journal Title: Eur J Pharmacol 606 Pages: 130-6
  • Peer Reviewed
• [Journal Article] Macrophage migration inhibitory factor activates hypoxia-inducible factor in a p53-dependent manner (2008)
  • Author(s): Oda S, Oda T, Nishi K, Takabuchi S, Wakamatsu T, Tanaka T, Adachi T, Fukuda K, Semenza GL, Hirota K
  • Journal Title: PLoS ONE 3 Pages: e2215
  • Peer Reviewed
• [Journal Article] LPS induces hypoxia-inducible factor 1 activation in macrophage-differentiated cells in a reactive oxygen species-dependent manner (2008)
  • Author(s): Nishi K, Oda T, Takabuchi S, Oda S, Fukuda K, Adachi T, Semenza GL, Shingu K, Hirota K
  • Journal Title: Antioxid Redox Signal 10 Pages: 983-96
  • Peer Reviewed
• [Journal Article] n-Propyl gallate activates hypoxia-inducible factor 1 by modulating intracellular oxygen-sensing systems (2008)
  • Author(s): Kimura M, Takabuchi S, Tanaka T, Murata M, Nishi K, Oda S, Oda T, Kanai M, Fukuda K, Kizaka-Kondoh S, Adachi T, Takabayashi A, Semenza GL, Hirota K
  • Journal Title: Biochem J 411 Pages: 97-105
  • Peer Reviewed
• [Journal Article] Hypoxia reduces the expression and anti-inflammatory effects of peroxisome proliferator-activated receptor-g in human proximal renal tubular cells (2007)
  • Author(s): Li X, Kimura H, Hirota K, Sugimoto H, Kimura N, Takahashi N, Fujii H, Yoshida H
  • Journal Title: Nephrol Dial Transplant 22 Pages: 1041-51
  • Peer Reviewed
• [Journal Article] Hypoxia and hypoxia-inducible factor-1 expression enhance osteolytic bone metastases of breast cancer (2007)
  • Author(s): Hiraga T, Kizaka-Kondoh S, Hirota K, Hiraoka M, Yoneda T
  • Journal Title: Cancer research 67 Pages: 4157-63
  • Peer Reviewed
• [Journal Article] Exhaled carbon monoxide levels change in relation to inspired oxygen fraction during general anesthesia (2007)
  • Author(s): Adachi T, Hirota K, Hara T, Sasaki Y, Hara Y
  • Journal Title: Anesth Analg 105 Pages: 696-9
  • Peer Reviewed
• [Presentation] The calcium channel blocker cilnidipine selectively suppresses hypoxia-inducible factor 1 activity in vascular endothelial and smooth muscle cells (2008)
  • Author(s): Takehiko Adachi, Seiko Oda, Satoshi Takabuchi , Kenichiro Nishi, Kiichi Hirota
  • Organizer: 2008Annual Meeting, American Society of Anesthesiologists
  • Place of Presentation: Orland
  • Year and Date: 20081018-22
• [Presentation] Macrophage migration inhibitory factor activates hypoxia-inducible factor 1 under hypoxic conditions in a p53-depenent manner (2008)
  • Author(s): Kiichi Hirota, Seiko Oda, Satoshi Takabuchi, Takuhiko Wakamatsu, Tomoharu Tanaka, Kazuhiko Fukuda
  • Organizer: Keystone symposia Molecular, cellular, physiologica, and phathogenic responses to hypoxia
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 2008-01-16