Application of galectin-3 in combined therapy of urogenital cancer

2008 Fiscal Year Final Research Report

• Project/Area Number: 19591854
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: The University of Tokushima
• Principal Investigator: FUKUMORI Tomoharu The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 講師 (10314874)
• Co-Investigator(Kenkyū-buntansha): KANAYAMA Hiro-omi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10214446) ; TAKAHASHI Masayuki 徳島大学, 医学部・歯学部附属病院, 講師 (50325255) ; NAKATSUJI Hiroyoshi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (50437638) ; OKA Natsuo 徳島大学, 医学部・歯学部附属病院, 助教 (40335790)
• Project Period (FY): 2007 – 2008
• Keywords: 泌尿器科癌 / ガレクチンー3 / 集学的治療 / 転移 / アポトーシス

Research Abstract

ガレクチンー3は腎細胞癌、膀胱癌の原発腫瘍のみならず腎細胞癌の転移巣でも強発現が認められ、腎細胞癌および膀胱癌患者では血清ガレクチンー3の強発現が認められた。前立腺癌細胞株(PC-3)、腎癌細胞株(KPK-1)および膀胱癌細胞株(J82)においてガレクチンー3の発現を約5分の1以下にノックアウトした細胞株を作成し、腎細胞癌、膀胱癌に対する放射線療法、シスプラチンによる化学療法とガレクチンー3の発現抑制による治療効果、アポトーシスへの影響を検討したところ、ガレクチンー3の発現を制御した腎細胞癌細胞株および膀胱癌細胞株では、細胞の増殖能が12%および14%低下し、放射線治療の感受性がそれぞれ18%、26%増加し、シスプラチンによる化学療法の感受性が15%、35%増加した。特に、ガレクチンー3ノックアウト細胞では、シスプラチン治療中に細胞質中のcytochrome cの発現が増加しcaspase-3が活性化されアポトーシスが誘導された。さらに、ガレクチンー3のブロッキング抗体を作成し今後の臨床的意義を検討する予定である。

Research Products

• [Journal Article] Laparoscopic findings of transverse testicular ectopia (2008)
  • Author(s): Takashashi M, Kurokawa Y, Nakanishi R, Nakatsuji H, lzaki H, Oka N, Fukumori T, Kanayama H
  • Journal Title: Urology 71・3, 547 Pages: e3-5
  • Peer Reviewed
• [Journal Article] Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions (2008)
  • Author(s): Nakatsuji H, Noriyuki Nishimura, Rie Yamamura, Hiro-omi Kanayama,Takuya Sasaki
  • Journal Title: Mel Cell Biol 28・10 Pages: 3324-35
  • Peer Reviewed
• [Journal Article] The Interaction of JRAB/MICAL-L2 with Rab8 and Rabl3 Coordinates the Assembly of Tight Junctions and Adherens Junctions (2008)
  • Author(s): Y amamura R, Noriyuki Nishimura, Hiroyoshi Nakatsuji, Seiji Arase, Takuya Sasaki
  • Journal Title: Mol Biol Cell 19・3 Pages: 971-83
  • Peer Reviewed
• [Journal Article] Involvement of Rabl3 and JRAB/MICAL-L2 in epithelial cell scattering (2008)
  • Author(s): Kanda I, N Nishimura, H Nakatsuji, R Yamamura, H Nakanishi T Sasaki
  • Journal Title: Oncogene 27・12 Pages: 1687-95
  • Peer Reviewed
• [Journal Article] Molecular Analysis of Transitional Cell Carcinoma of Upper Urinary Tract Using cDNA Microarray (2008)
  • Author(s): Essam Elsamman, Ashraf A. Ewis, iNermin Ali, Mohamed D. Saleem, Mohamed Gadelmoula, Yoshinobu Baba, Hiro-omi Kanayama
  • Journal Title: ARAB Journal of Urology 6・1 Pages: 48-55
  • Peer Reviewed
• [Journal Article] Strategy for repeat prostate biopsy : predictors of positive biopsy and additional biopsy location (2008)
  • Author(s): Katto K, Masayuki Takahashi, Ryuichi Taue, Hiroyoshi Nakatsuji, Tomoteru Kishimoto, Hirofumi Izaki, Natsuo Oka, Tomoharu Fukumori, Masa-Aki Nishitani, Hiro-Omi Kanayama
  • Journal Title: Urol Int 81 Pages: 173-178
  • Peer Reviewed
• [Journal Article] Bladder Cancer Cell Invasion Is Enhanced by Cross-Talk with Fibroblasts Through Hepatocyte Growth Factor (2008)
  • Author(s): Sakaki M, Oka N, Nakanishi R, Yamaguchi K, Fukumori T, Kanayama H
  • Journal Title: The Journal of Medical Investigation 55 Pages: 127-132
  • Peer Reviewed
• [Journal Article] Prognostic significance of serum hepatocyte growth factor in clear cell renal cell carcinoma : comparison with serum vascular endothelial growth factor (2008)
  • Author(s): Tanimoto S, Fukumori T, Gad El-Moula, Shiirevnyamba A, Kinouchi S, Koizumi T, Nakanishi R, Yamamoto Y, Taue R, Yamaguchi K, Nakatsuji H, Kishimoto T, Izaki H, Oka N. Takahashi M, Kanayama H
  • Journal Title: The Journal of Medical Investigation 55 Pages: 106-111
  • Peer Reviewed
• [Journal Article] Bladder Cancer Cell Invasion Is Enhanced by Cross-Talk with Fibroblatss Through Hepatocyte Growth Factor (2007)
  • Author(s): Wang P, Nishitani M, Tanimoto S, Kishimoto T, Fukumori T, Takahashi M, Kanayama H
  • Journal Title: UROLOGY 69・4 Pages: 780-784
  • Peer Reviewed
• [Journal Article] Gene expression profile ine squamous cell calcinoma of the urinary bladder using complementary deoxyribonucleic acid microarray (2007)
  • Author(s): Ashiraf A, El-Samman E, Ali N, Kajimoto K, Shinohara Y, Ishikawa M, Kanayama H, Baba Y
  • Journal Title: Urologic Oncology 25 Pages: 120-127
  • Peer Reviewed
• [Journal Article] Prevalence of renal cell carcinoma : A nation-wide survey in Japan, 2002 (2007)
  • Author(s): Marumo K, Kanayama H, NiyaoN, Nakazawa H, Ozono S, Horie S, Nagamori S, Igarashi T, Hasegawa M, Kimura G, Nakao M, Nakamoto T, Naito S
  • Journal Title: International Journal of Urology 14 Pages: 479-482
  • Peer Reviewed
• [Journal Article] Massive Leiomyosarcoma of the spermatig cord (2007)
  • Author(s): Taue R, Izaki H, Nakanishi R, Yamaguchi K, Nakatsuji H, Kishimoto T, Oka N, Fukumori T, Takahashi M, Nishitani M, Kanayama H
  • Journal Title: Acta Urol. Jpn 53 Pages: 497-500
  • Peer Reviewed
• [Journal Article] The role of galectin-3 in cancer drug resistance (2007)
  • Author(s): Fukumori T, Kanayama H, Raz A
  • Journal Title: Drug Resistance Upstates 10 Pages: 101-108
  • Peer Reviewed
• [Journal Article] Does granular cell renal cell carcinoma exist? MoIecular and histological reclassfication (2007)
  • Author(s): Yang XJ, M Takahashi, KT Schafernak, M S Tretiakova, J Sugimura, N J Vogelzang, B T The
  • Journal Title: Histopathology 50・5 Pages: 678-680
  • Peer Reviewed
• [Journal Article] 前立腺癌の抗がん剤耐性とアポトーシス調節機構 (2007)
  • Author(s): 福森知治、岡夏生、中西良一、楠原義人、山口邦久、田上隆一、中達弘能、岸本大輝、井崎博文、高橋正幸、西谷真明、金山博臣
  • Journal Title: 西日本泌尿器科 69巻4号 Pages: 176-182
  • Peer Reviewed
• [Journal Article] 尿路性器癌における抗がん剤耐性機序(総説) (2007)
  • Author(s): 福森知治、金山博臣
  • Journal Title: 西日本泌尿器科 69巻12号 Pages: 693-697
  • Peer Reviewed
• [Presentation] Laparoscopic nephropexy for sympromatic nephroptosis (2008)
  • Author(s): Nakatsuji H
  • Organizer: World Congress of Endourology & SWL
  • Place of Presentation: Shanghai
  • Year and Date: 2008-12-01
• [Presentation] Galectin-3 is overexpressed in renal cell carcinoma and it regulates immune suppression through the induction of appoptosis of Tcells (2008)
  • Author(s): Fukumori T
  • Organizer: AUA Annual Meeting
  • Place of Presentation: Frolida
  • Year and Date: 2008-05-18
• [Presentation] 膀胱癌細胞におけるuPAinhibitorによるTRAIL誘導アポトーシスの増強効果について (2008)
  • Author(s): 岡夏生
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-04-26
• [Presentation] The expression of claudin-7 in transitional cell carsinoma of the urinary bladder : a quantitative reverse transcriptase-polymerase chain reaction analysis (2007)
  • Author(s): Kanayama H
  • Organizer: SIU
  • Place of Presentation: Paris
  • Year and Date: 20070902-06
• [Presentation] Galectin-3 overexpressed in renal cell carsinoma and it regulates immune suppression through the induction of apoptosis of cytotoxic T cells (2007)
  • Author(s): Fukumori T
  • Organizer: SIU
  • Place of Presentation: Paris
  • Year and Date: 20070902-06
• [Presentation] Interaction between IGF-1, HGF and uPA/uPAR in migration and invasion of bladdercancer cells (2007)
  • Author(s): 岡夏生
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2007-10-04