Mechanistic analysis of nonmyeloablative allogeneic cell therapy for murine renal cancer and enhancement of antitumor effects

2008 Fiscal Year Final Research Report

• Project/Area Number: 19591856
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kyushu University
• Principal Investigator: MASATOSHI Eto Kyushu University, 大学病院, 講師 (90315078)
• Co-Investigator(Kenkyū-buntansha): TATSUGAMI Katsunori 九州大学, 大学病院, 助教 (90380617) ; HARANO Masahiko 九州大学, 大学病院, 助教 (90380451) ; YOSHIKAI Yasunobu 九州大学, 生体防御医学研究所, 教授 (90158402)
• Project Period (FY): 2007 – 2008
• Keywords: renal cell carcinoma / bone marrow transplantation / mixed chimerism / tumor antigen peptide / GVT effect

Research Abstract

我々は既報のマウス腎癌に対するミニ移植モデル(Cancer Res, 65 : 10032, 2005)に続いて、今回、マウス膀胱癌に対するミニ移植モデルを確立した。末梢血および腫瘍局所におけるドナー細胞の実際の動きをより詳細に検討した結果、ドナー由来のCD4陽性T細胞が抗腫瘍効果の誘導に重要であることを証明した。また、ミニ移植において、腫瘍抗原ペプチドを用いたドナーの前感作を行い、腫瘍抗原特異的な免疫反応を加えることで、抗腫瘍効果の増強が誘導できることを示し、臨床応用の可能性が示唆された。

Research Products

• [Journal Article] Donor CD4 T cells are critical in allogeneic stem cell transplantation against murine solid tumor (2009)
  • Author(s): Kamiryo Y, Eto M, Yamada H, YajimaT, Harano M, Takeuchi A, Tatsugami K,Hamaguchi M, Naito S, Yoshikai Y.
  • Journal Title: Cancer Res (in press)
  • Peer Reviewed
• [Journal Article] Allogeneic cell therapy from immunized donors with tumor antigen peptide enhances the antitumor effect after cyclophosphamide-using nonmyeloablative (2009)
  • Author(s): Hamaguchi M, Eto M, Kamiryo Y,Takeuchi A, Harano M, Tatsugami K,Teshima T, Harada M, Yoshikai Y, Naito S.
  • Journal Title: allogeneichematopoietic cell transplantation inmice. Cancer Sci 100 Pages: 138-144
  • Peer Reviewed
• [Journal Article] Posttransplant administration of cyclophosphamide and donor lymphocyte infusion induces potent antitumor immunity to solid tumor (2008)
  • Author(s): Eto M, Kamiryo Y, Takeuchi A, HaranoM, Tatsugami K, Harada M, Kiyoshima K,Hamaguchi M, Teshima T, Tsuneyoshi M,Yoshikai Y, and Naito S.
  • Journal Title: Clin Cancer Res 14 Pages: 2833-2840
  • Peer Reviewed
• [Presentation] Vaccination of donors with tumor antigen peptide enhances the antitumor effects on renal tumors in recipient mice with nonmyeloablative allogeneic hemopoietic cell transplantation (2008)
  • Author(s): Eto M
  • Organizer: American Urological Association Annual Meeting 2008
  • Place of Presentation: Orlando, USA
  • Year and Date: 2008-05-18
• [Presentation] Vaccination of donors with tumor antigen peptides enhances the antitumor effect on solid tumors in recipient mice with nonmyeloablative allogeneic hemopoietic cell transplantation (2007)
  • Author(s): Eto M
  • Organizer: American Urological Association Annual Meeting 2007
  • Place of Presentation: Anaheim, USA
  • Year and Date: 2007-05-20