The Role of thrombin receptor on urinary stone

2009 Fiscal Year Final Research Report

• Project/Area Number: 19591873
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kanazawa Medical University
• Principal Investigator: SUZUKI Koji Kanazawa Medical University, 医学部, 教授 (70064615)
• Co-Investigator(Kenkyū-buntansha): MIYAZAWA Katsuhito 金沢医科大学, 医学部, 准教授 (60219772) ; MORIYAMA Manabu 金沢医科大学, 医学部, 講師 (50278131)
• Project Period (FY): 2007 – 2009
• Keywords: Thrombin / Protease-activated receptor (PAR) / Urolithiasis / Calcium oxalate

Research Abstract

We evaluated the expression of mRNA and protein of Protease-activated receptor (PAR) as thrombin receptor in renal epithelial cells, and whether or not oxalate initiate signal transduction via this pathway. Gene and protein expression was clearly shown in HK-2 cells, which originated human tubular epithelium by PCR and Western blotting. Oxalate exposure caused up-regulation of intra-cellular calcium ion and Interleukin 6 production.

Research Products

• [Journal Article] cDNA macroarray analysis of genes in renal epithelial cells exposed to calcium oxalate crystals. (2009)
  • Author(s): Miyazawa K, Aihara K, Ikeda R, Moriyama MT, Suzuki K.
  • Journal Title: Urol Res. 37 Pages: 27-33
  • Peer Reviewed
• [Journal Article] Inhibitions of urinary oxidative stress and renal calcium level by an extract of Quercus salicina Blume/Quercus stenophylla Makino in a rat calcium oxalate urolithiasis model. (2009)
  • Author(s): Moriyama MT, Suga K, Miyazawa K, Tanaka T, Higashioka M, Noda K, Oka M, Tanaka M, Suzuki K.
  • Journal Title: Int J Urol. 16 Pages: 397-401
  • Peer Reviewed
• [Journal Article] 尿中高分子物質から再発予防を考える. (2008)
  • Author(s): 宮澤克人, 飯田如, 鈴木孝治
  • Journal Title: 泌尿器外科 21 Pages: 697-702
• [Journal Article] Reduction in oxalate-induced renal tubular epithelial cell injury by an extract from Quercus salicina Blume/Quercus stenophylla Makino. (2007)
  • Author(s): Moriyama MT, Miyazawa K, Noda K, Oka M, Tanaka M, Suzuki K.
  • Journal Title: Urol Res. 35 Pages: 295-300
  • Peer Reviewed
• [Journal Article] ゲノム解析からのアプローチ. (2007)
  • Author(s): 宮澤克人, 鈴木孝治
  • Journal Title: Pharma Medica 25 Pages: 21-24
• [Journal Article] 尿中高分子物質 (2007)
  • Author(s): 宮澤克人, 鈴木孝治
  • Journal Title: 最新医 別冊52 Pages: 161-166
• [Journal Article] 飲水による再発予防 (2007)
  • Author(s): 宮澤克人, 鈴木孝治
  • Journal Title: 臨泌 61 Pages: 477-481
• [Presentation] 尿路結石症における尿中高分子物質について (2009)
  • Author(s): 宮澤克人
  • Organizer: 日本泌尿器科学会中部総会
  • Place of Presentation: 金沢
  • Year and Date: 2009-10-30
• [Presentation] (2009)
  • Author(s): 宮澤克人, 飯田如, 森山学, 鈴木孝治
  • Organizer: 日本泌尿器科学会中部総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-04-24
• [Presentation] 腎尿細管上皮細胞におけるProtease-activated receptorの発現と機能について (2008)
  • Author(s): 宮澤克人, 菅幸大, 森山学, 鈴木孝治
  • Organizer: 北陸泌尿器科Basic research meeting
  • Place of Presentation: 金沢
  • Year and Date: 2008-02-12
• [Book] マイクロアレイ. 尿路結石のすべて (2008)
  • Author(s): 宮澤克人, 鈴木孝治
  • Total Pages: 205
  • Publisher: 医学書院