Neuron-Like Differentiation and Selective Ablation of Undifferentiated Embryonic Stem Cells Containing Suicide Gene with Oct-4 Promoter

2008 Fiscal Year Final Research Report

• Project/Area Number: 19592012
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Gifu University
• Principal Investigator: HARA Akira Gifu University, 大学院・医学系研究科, 教授 (10242728)
• Project Period (FY): 2007 – 2008
• Keywords: 胚性肝細胞 / ES細胞 / 神経組織再生 / 奇形腫 / 自殺遺伝子 / thymidine kinase / ganciclovir human

Research Abstract

研究成果が5編の論文として国際雑誌に掲載された。これら論文には、基礎研究としての海馬神経細胞、眼球光彩色素細胞の研究も含まれる。OCT-4遺伝子プロモーター下流に自殺遺伝子thymidine kinase を挿入したES細胞(OCT-TK-ES細胞)を3次元培養し、ganciclovir投与により選択的に未分化ES細胞の増殖を抑制し、神経分化蛋白発現の変化を検索し、ganciclovir投与により未分化細胞成分のみが除去され、神経分化を示すことが示された。

Research Products

• [Journal Article] Spatiotemporal expression of Hsp20 and its phosphorylation in hippocampal CA1 pyramidal neurons following transient forebrain ischemia (2009)
  • Author(s): Niwa M, Hara A, Taguchi A, Aoki H, Kozawa O, Mori H
  • Journal Title: Neurol Res (in press)
  • Peer Reviewed
• [Journal Article] Marked increases in hippocampal neuron indoleamine 2, 3-dioxygenase via IFN-γ-independent pathway following transient global ischemia inmouse (2009)
  • Author(s): Hoshi M, Saito K, Murakami Y, Taguchi A, Fujigaki H, Tanaka R, Takemura M, Ito H, Hara A, Seishima M.
  • Journal Title: Neurosci Res 63 Pages: 194-198
  • Peer Reviewed
• [Journal Article] Localization and spatiotemporal expression of IDO following transient forebrain ischemia in gerbils (2008)
  • Author(s): Taguchi A, Hara A, Saito K, Hoshi M, Niwa M, Seishima M, Mori H.
  • Journal Title: Brain Res 1217 Pages: 78-85
  • Peer Reviewed
• [Journal Article] Transplantation of melanocytes into iris: Method for iris repigmentation (2008)
  • Author(s): Aoki H, Yoshida H, Hara A, Suzuk T, Kunisada T.
  • Journal Title: Transplantation 85 Pages: 492-494
  • Peer Reviewed
• [Journal Article] Neuron-like Differentiation and Selective Ablation of Undifferentiated Embryonic Stem Cells Containing Suicide Gene with Oct-4 Promoter (2008)
  • Author(s): Hara A, Aoki H, Taguchi A, Niwa M, Yamada Y, Kunisada T, Mori H
  • Journal Title: Stem Cells Dev 17 Pages: 619-628
  • Peer Reviewed
• [Journal Article] Iris as a recipient tissue for pigment cells: organized in vivo differentiation of melanocytes and pigmented epithelium derived from embryonic stem cells in vitro (2008)
  • Author(s): Aoki H, Hara A, Motohashi T, Chem H, Kunisada T
  • Journal Title: Dev Dynam 237 Pages: 2394-2404
  • Peer Reviewed
• [Presentation] Neuronal differentiation and control of teratoma formation in transplanted embryonic stem cells using retinal neurodegenerative mouse model (2009)
  • Author(s): 原明
  • Organizer: Symposium : Molecular pharmacological understandings of neurodegenerative disorders. The spring international convention, organized by The Pharmaceutical Society of Korea
  • Place of Presentation: 韓国, Daejeon
  • Year and Date: 2009-05-07
• [Presentation] Methotrexate induces neuronal differentiation of human embryonic stem (ES) cells transplanted into nude mouse retina (2008)
  • Author(s): 原明、田口紋子、山田泰広、森秀樹
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] Selective ablation of undifferentiated embryonic stem cells containing suicide gene with Oct-4 promoter (2007)
  • Author(s): 原明、田口紋子、尾山武、山田泰広、森秀樹
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-02
• [Presentation] Supression of tumorigenicity and induction of neuronal differentiation in embryonic stem cells transplanted in the adult mouse retina by folate antagonist methotrexate (2007)
  • Author(s): 原明
  • Organizer: AACR Annual Meeting
  • Place of Presentation: Los Angeles
  • Year and Date: 2007-04-15
• [Book] OCT-3/4 Expressing Cells as Cancer Stem Cells in Human Immature Teratoma : Cancer Differentiation Potential(New Cell Differentiation Research Topics)(Saitama H) (2008)
  • Author(s): Hara A, Oka N, Aoki H, Taguchi A, Yamada Y, Niwa M, Mori H
  • Total Pages: 1-6
  • Publisher: Nova Science Publish