Novel genetic analysis in Behcet's disease

2009 Fiscal Year Final Research Report

• Project/Area Number: 19592030
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Yokohama City University
• Principal Investigator: NISHIDE Tadayuki Yokohama City University, 医学部, 助教 (90347344)
• Co-Investigator(Kenkyū-buntansha): MIZUKI Nobuhisa 横浜市立大学, 医学研究科, 教授 (90336579) ; ITO Norihiko 横浜市立大学, 医学部, 助教 (80264654)
• Co-Investigator(Renkei-kenkyūsha): OHNO Shigeaki 北海道大学, 大学院・医学研究科, 特任教授 (50002382) ; INOKO Hidetoshi 東海大学, 医学部, 教授 (10101932)
• Project Period (FY): 2007 – 2009
• Keywords: ゲノム / 遺伝子 / マイクロサテライト / SNP / 多因子性遺伝疾患

Research Abstract

To identify susceptibility genes for Behcet's disease (BD), we performed a genome-wide association study of BD achieved through three rounds of pooled DNA screening using a comprehensive set 23,465 microsatellite markers in 300 Japanese cases and 300 controls, and identified the six best positively associated microsatellites with BD.

Research Products

• [Journal Article] Genome-wide association studies identify IL23R/IL12RB2 and IL10 as Behcet's disease susceptibility loci. (2010)
  • Author(s): Mizuki N, Meguro A, Ota M, Ohno S, Shiota T, Kawagoe T, Ito N, Kera J, Okada E, Yatsu K, Song YW, Lee EB, Kitaichi N, Namba K, Horie Y, Takeno M, Sugita S, Mochizuki M, Bahram S, Ishigatsubo Y, Inoko H
  • Journal Title: Nature Genetics (in press)
• [Journal Article] Analysis of microsatellite polymorphisms within the GLC1F locus in Japanese patients with normal tension glaucoma. (2010)
  • Author(s): Murakami K, Meguro A, Ota M, Shiota T, Nomura N, Kashiwagi K, Mabuchi F, Iijima H, Kawase K, Yamamoto T, Nakamura M, Negi A, Sagara T, Nishida T, Inatani M, Tanihara H, Aihara M, Araie M, Fukuchi T, Abe H, Higashide T, Sugiyama K, Kanamoto T, Kiuchi Y, IwMurakami K, Meguro A, Ota M, Shi
  • Journal Title: Mol Vis 16 Pages: 462-466
• [Journal Article] Genetics of Behcet's disease inside and outside the MHC. (2010)
  • Author(s): Meguro A, Inoko H, Ota M, Katsuyama Y, Oka A, Okada E, Yamakawa R, Yuasa T, Fujioka T, Ohno S, Bahram S, Mizuki N
  • Journal Title: Ann Rheum Dis 69 Pages: 747-754
• [Journal Article] Investigation of the association between TLR2 gene polymorphisms and Behcet's disease in Japanese patients. (2009)
  • Author(s): Tomiyama R, Meguro A, Ota M, Katsuyama Y, Nishide T, Uemoto R, Iijima Y, Ohno S, Inoko H, Mizuki N
  • Journal Title: Human Immunol 70(1) Pages: 41-44
• [Journal Article] Association of TLR4 polymorphisms with Behcet's disease in a Korean population. (2009)
  • Author(s): Horie Y, Meguro A, Ota M, Kitaichi N, Katsuyama Y, Takemoto Y, Namba K, Yoshida K, Song YW, Park KS, Lee EB, Inoko H, Mizuki N, Ohno S
  • Journal Title: Rheumatology 48(6) Pages: 638-642
• [Journal Article] Association of toll-like receptor 2 gene polymorphisms with normal tension glaucoma. (2009)
  • Author(s): Nakamura J, Meguro A, Ota M, Nomura E, Nishide T, Kashiwagi K, Mabuchi F, Iijima H, Kawase K, Yamamoto T, Nakamura M, Negi A, Sagara T, Nishida T, Inatani M, Tanihara H, Aihara M, Araie M, Fukuchi T, Abe H, Higashide T, Sugiyama K, Kanamoto T, Kiuchi Y, INakamura J, Meguro A, Ota M, Nomu
  • Journal Title: Mol Vis 15 Pages: 2905-2910
• [Journal Article] Lack of association between toll-like receptor 4 gene polymorphisms and sarcoidosis-related uveitis in Japan. (2009)
  • Author(s): Asukata Y, Ota M, Meguro A, Katsuyama Y, Ishihara M, Namba K, Kitaichi N, Morimoto S, Kaburaki T, Ando Y, Takenaka S, Inoko H, Ohno S, Mizuki N
  • Journal Title: Mol Vis 15 Pages: 2673-2682
• [Journal Article] New susceptibility locus for high myopia is linked to the uromodulin-like 1 (UMODL1) gene region on chromosome 21q22. 3. (2009)
  • Author(s): Nishizaki R, Ota M, Inoko H, Meguro A, Shiota T, Okada E, Mok J, Oka A, Ohno S, Mizuki N
  • Journal Title: Eye 23(1) Pages: 222-229
• [Journal Article] Investigation of the association between the GLC3A locus and normal tension glaucoma in Japanese patients by microsatellite analysis. (2009)
  • Author(s): Kamio Y, Meguro A, Ota M, Nomura N, Kashiwagi K, Mabuchi F, Iijima H, Kawase K, Yamamoto T, Nakamura M, Negi A, Sagara T, Nishida T, Inatani M, Tanihara H, Aihara M, Araie M, Fukuchi T, Abe H, Higashide T, Sugiyama K, Kanamoto T, Kiuchi Y, Iwase A, Ohno SKamio Y, Meguro A
  • Journal Title: Clinical Ophthalmol 3 Pages: 183-188
• [Journal Article] Association of microsatellite polymorphisms of the GPDS1 locus with normal tension glaucoma in the Japanese population. (2009)
  • Author(s): Nakamura K, Ota M, Meguro A, Nomura N, Kashiwagi K, Mabuchi F, Iijima H, Kawase K, Yamamoto T, Nakamura M, Negi A, Sagara T, Nishida T, Inatani M, Tanihara H, Aihara M, Araie M, Fukuchi T, Abe H, Higashide T, Sugiyama K, Kanamoto T, Kiuchi Y, Iwase A, OhnNakamura K, Ota M, Meg
  • Journal Title: Clinical Ophthalmol 3 Pages: 307-312
• [Journal Article] Association of toll-like receptor 4 gene polymorphisms with normal tension glaucoma. (2008)
  • Author(s): Shibuya E, Meguro A, Ota M, Kashiwagi K, Mabuchi F, Iijima H, Kawase K, Yamamoto T, Nakamura M, Negi A, Sagara T, Nishida T, Inatani M, Tanihara H, Aihara M, Araie M, Fukuchi T, Abe H, Higashide T, Sugiyama K, Kanamoto T, Kiuchi Y, Iwase A, Ohno S, Inoko Shibuya E
  • Journal Title: Invest Opthalmol Vis Sci 49(10) Pages: 4453-4457
• [Journal Article] Association of the toll-like receptor 4 gene polymorphisms with Behcet's disease. (2008)
  • Author(s): Meguro A, Ota M, Katsuyama Y, Oka A, Ohno S, Inoko H, Mizuki N
  • Journal Title: Ann Rheum Dis 67(5) Pages: 725-727
• [Journal Article] Four-digit allele genotyping of HLA-A and HLA-B genes in Japanese patients with Behcet's disease (BD) by a PCR-SSOP-luminex method and stratification analysis according to each major symptom of BD. (2008)
  • Author(s): Kamiishi T, Itoh Y, Meguro A, Nishida T, Sasaki S, Nanba K, Ohno S, Inoko H, Mizuki N
  • Journal Title: Nippon Ganka Gakkai Zasshi 112(5) Pages: 451-458
• [Journal Article] Association of major histocompatibility complex class I chain-related gene A and HLA-B alleles with Behcet's disease in Turkey. (2007)
  • Author(s): Mizuki N, Meguro A, Tohnai I, Gul A, Ohno S, Mizuki N
  • Journal Title: Jpn J Ophthalmol 51(6) Pages: 431-436
• [Journal Article] A single nucleotide polymorphism analysis of the LAMA1 gene in Japanese patients with high myopia. (2007)
  • Author(s): Sasaki S, Ota M, Meguro A, Nishizaki R, Okada E, Mok J, Kimura T, Oka A, Katsuyama Y, Ohno S, Inoko H, Mizuki N
  • Journal Title: Clinical Opthalmol 1(3) Pages: 289-295
• [Journal Article] Lack of association with high myopia and the MYP2 locus in the Japanese population by high resolution microsatellite analysis on chromosome 18. (2007)
  • Author(s): Yamane T, Mok J, Oka A, Okada E, Nishizaki R, Meguro A, Yonemoto J, Kulski JK, Ohno S, Inoko H, Mizuki N
  • Journal Title: Clinical Opthalmol 1(3) Pages: 311-316
• [Presentation] Molecular genetic studies on Behcet's disease in Japan. (2009)
  • Author(s): Meguro A, Mizuki N, Ohno S
  • Organizer: The 10th International Ocular Inflammation Society Congress
  • Place of Presentation: Praque, Czech Republic
  • Year and Date: 20090600
• [Presentation] Whole genome association study on Behcet's disease. (2007)
  • Author(s): Meguro A, Mizuki N
  • Organizer: Japan and Korea Joint Meeting on Behcet's disease
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20070700