Mechanism of angiogenesis induced by adipose-derived stem cells

2008 Fiscal Year Final Research Report

• Project/Area Number: 19592080
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Nippon Medical School
• Principal Investigator: MIZUNO Hiroshi Nippon Medical School, 医学部, 准教授 (80343606)
• Co-Investigator(Kenkyū-buntansha): OKI Koichiro 日本医科大学, 医学部, 講師 (90291715) ; KOIKE Sachiko 日本医科大学, 医学部, 助教 (90386227) ; HYAKUSOKU Hiko 日本医科大学, 医学研究科, 教授 (00165135)
• Research Collaborator: ONO Shimpei 日本医科大学, 大学院生
• Project Period (FY): 2007 – 2008
• Keywords: 脂肪組織幹細胞 / 血管新生 / 増殖因子 / 血管内皮細胞 / 再生医学 / 組織工学

Research Abstract

野生型C57BL/6-Tgマウスの下肢虚血モデルに対し、GFP遺伝子導入C57BL/6-Tgマウス由来脂肪組織幹細胞(以下ASCs)を下肢筋肉内に注入投与した。経時的に肉眼的観察、皮膚温測定、血管造影、各種血管成長因子の発現を調査下。血管造影では実験群において豊富な血管新生像を呈した。GFPを発現する血管内皮細胞は全体の10-15%であった。血管成長因子の発現の程度は細胞移植後早期より上昇し、移植後後期まで持続した。以上より、血管再生効果は血管成長因子の効果が大であると推測された。

Research Products

• [Journal Article] Adipose-derived stem cells for tissue repair and regeneration : Ten years of research and a literature review (2009)
  • Author(s): Mizuno H
  • Journal Title: J Nippon Med Sch 76 Pages: 56-66
  • Peer Reviewed
• [Journal Article] Accelerate wound healing in healing-impaired db/db mice by autologous adipose tissue-derived stromal cells conbined with atelocollagen matrix (2009)
  • Author(s): Nambu M, Kishimoto S, Nakamura S, Mizuno H, Yanagibayashi S, Yamamoto N, Azuma R, Nakamura S, Kiyosawa T, Ishihara M and Kanatani Y
  • Journal Title: Ann Plast Surg 62 Pages: 317-321
  • Peer Reviewed
• [Journal Article] 脂肪組織由来幹細胞注入と脂肪注入の違いによる乳房増大術の現状と課題について (2008)
  • Author(s): 水野博司
  • Journal Title: 日美外報 30(3) Pages: 161-164
  • Peer Reviewed
• [Journal Article] Periodontal tissue regeneration with adipose-derived stem cells (2008)
  • Author(s): Tobita M, Uysal AC, Ogawa R, Hyakusoku H and Mizuno H
  • Journal Title: Tissue Eng 14(6) Pages: 945-953
  • Peer Reviewed
• [Journal Article] In vivo adipose tissue regeneration by adipose-derived stromal cells isolated from GFP transgenic mice (2008)
  • Author(s): Mizuno H, Itoi Y, Kawahara S, Ogawa R, Akaishi S and Hyakusoku H
  • Journal Title: Cells Tissues Organs 187(3) Pages: 177-185
  • Peer Reviewed
• [Journal Article] Improved viability of random pattern skin flaps through the use of adipose-derived stem cells (2008)
  • Author(s): Lu F, Mizuno H, Uysal AC, Cai X, Ogawa R and Hyakusoku H
  • Journal Title: Plast Reconstr Surg 121(1) Pages: 50-58
  • Peer Reviewed
• [Journal Article] 皮下脂肪-再生医療の新たなる細胞源 (2007)
  • Author(s): 水野博司
  • Journal Title: 日医大誌 3(4) Pages: 183-186
• [Journal Article] Enhanced healing of mitomycin C-treated wounds in rats using inbred adipose tissue-derived stromal cells within an atelocollagen matrix (2007)
  • Author(s): Nambu M, Ishihara M, Nakamura S, Mizuno H, Yanagibayashi S, Kanatani Y, Hattori H, Takase B, Ishizuka T, Kishimoto S, Amano Y, Yamamoto N, Azuma R and Kiyosawa T
  • Journal Title: Wound Repair Regen 15(4) Pages: 505-510
  • Peer Reviewed
• [Journal Article] The effect of adipose-derived stem cells on ischemia reperfusion
  • Author(s): Uysal AC, Ogawa R, Tobita M, Hyakusoku H and Mizuno H
  • Journal Title: injury : immunohistochemical and ultrastructural evaluation Plast Reconstr Surg (in press)
  • Peer Reviewed
• [Journal Article] Comparison of readily available scaffolds for adipose tissue engineering using adipose-derived
  • Author(s): Itoi Y, Takatori M, Hyakusoku H and Mizuno H
  • Journal Title: J Plast Reconstr Aesthet Surg (in press)
  • Peer Reviewed
• [Journal Article] Prefabrication of tissue engineered bone grafts : an experimental study
  • Author(s): Okuda T, Uysal AC, Tobita M, Hyakusoku H and Mizuno H
  • Journal Title: Ann Plast Surg (in press)
  • Peer Reviewed
• [Presentation] Adipose-derived stem cells for tissue repair and regeneration 9th National Conference of Burn (2009)
  • Author(s): Mizuno H
  • Organizer: Reconstructive and Plastic Surgery
  • Place of Presentation: ハノイ
  • Year and Date: 2009-03-03
• [Presentation] 幹細胞より誘導した再生骨に対しprefabrication手法を用いて血管導入された組織移植に関する研究 (2009)
  • Author(s): 奥田貴久、A. Cagri Uysal、Hakan Orbay、河邊京子、小野真平、糸井由里恵、百束比古、水野博司
  • Organizer: 第1回日本創傷外科学会
  • Place of Presentation: 東京
  • Year and Date: 2009-01-17
• [Presentation] Adipose derived stem cells accelerate primary nerve repair (2008)
  • Author(s): Mizuno H, Uysal AC, Orbay H, Okuda T and Hyakusoku H
  • Organizer: 6^<th> Annual Meeting of International Federation of Adipose Therapeutics and Science
  • Place of Presentation: oulouse, France 2008
  • Year and Date: 2008-10-16
• [Presentation] prefabricated flapを用いた再生培養骨への血管付加 (2008)
  • Author(s): 奥田貴久、A. Cagri Uysal、糸井由里恵、河邊京子、小野真平、Hakan Orbay、百束比古、水野博司
  • Organizer: 第17回日本形成外科学会基礎学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-02
• [Presentation] Flap prefabrication technique as a tool for inducing angiogenesis in tissue engineering (2008)
  • Author(s): Okuda T, Tobita M, Uysal AC, Ogawa R, Hyakusoku H and Mizuno H
  • Organizer: 3^<rd> Annual Meeting of the Plastic Surgery Research Council
  • Place of Presentation: Splingfield, IL
  • Year and Date: 2008-05-30
• [Presentation] Clinical perspective of the stem cell therapy in the field of plastic surgery (2008)
  • Author(s): Mizuno H, Uysal AC, Tobita M, Itoi Y, Hyakusoku H and Nambu H
  • Organizer: The 9^<th> Japan-Korean Congress of Plastic and Reconstructive Surgery
  • Place of Presentation: 沖縄
  • Year and Date: 2008-02-22
• [Presentation] Therapeutic strategy for chronic wound by stem cells or controlled-release of basic fibroblast growth factors (2008)
  • Author(s): Mizuno H, Hyakusoku H, Miyamoto M and Tabata Y
  • Organizer: International Conference on Advances in Bioresorbable Biomaterials for Tissue Engineering
  • Place of Presentation: Singapore
  • Year and Date: 2008-01-06
• [Presentation] The effect of stem cells on acute wound healing Tissue Engineering (2007)
  • Author(s): Uysal AC, Mizuno H, Tobita M and Hyakusoku H
  • Organizer: International & Regenerative Medicine Society Asia-Pacific Chapter Meeting 2007
  • Place of Presentation: 東京
  • Year and Date: 2007-12-05
• [Presentation] Angiogenic potential of adipose-derived stem cells in skin flap model (2007)
  • Author(s): Mizuno H, Uysal AC, Lu F, Ogawa R and Hyakusoku H
  • Organizer: Tissue Engineering International & Regenerative Medicine Society Asia-Pacific Chapter Meeting 2007
  • Place of Presentation: 東京
  • Year and Date: 2007-12-05
• [Presentation] The effect of stem cells on acute wound healing (2007)
  • Author(s): Uysal AC, Mizuno H, Nambu M, Tobita M and Hyakusoku H
  • Organizer: 5^<th> Annual Meeting of International Federation of Adipose Therapeutics and Science
  • Place of Presentation: Indianapolis, Indiana
  • Year and Date: 2007-10-19
• [Presentation] 拡張された皮弁に対する脂肪組織幹細胞の影響 (2007)
  • Author(s): A. Cagri Uysal、水野博司、飛田護邦、糸井由里恵、百束比古
  • Organizer: 第16回日本形成外科学会基礎学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2007-10-11
• [Presentation] 再生医療の現状と脂肪組織幹細胞の有用性 (2007)
  • Author(s): 水野博司
  • Organizer: 第4回日本獣医内科学アカデミー学術大会
  • Place of Presentation: 東京
  • Year and Date: 2007-08-12
• [Presentation] Adipose derived stem cells as a novel source of cell-based therapy in vivo (2007)
  • Author(s): Mizuno H, Uysal AC, Tobita M, Itoi Y, Ogawa R, Nambu M, and Hyakusoku H
  • Organizer: The 14^<th> international congress of the international confederation for plastic, reconstructive and aesthetic surgery
  • Place of Presentation: Berlin
  • Year and Date: 2007-06-28
• [Presentation] The effect of stem cells on skin flap models : random skin flap, prefabricated skin flap, ischemia reperfusion injury model, expanded skin flap (2007)
  • Author(s): Uysal AC, Mizuno H, Ogawa R, Lu F, Tobita M and Hyakusoku H
  • Organizer: The 14^<th> international congress of the international confederation for plastic, reconstructive and aesthetic surgery
  • Place of Presentation: Berlin
  • Year and Date: 2007-06-28
• [Book] 脂肪組織由来幹細胞「遺伝子医学MOOK別冊 進み続ける細胞移植治療の実際-再生医療の実現に向けた科学・周辺要素の理解-(上巻)細胞移植治療に用いる細胞とその周辺科学・技術」(編集 : 田畑泰彦) (2008)
  • Author(s): 水野博司
  • Total Pages: 77-80
  • Publisher: メディカルドウ(分担)
• [Book] Tissue engineering using adipose-derived stem cells harvested from GFP transgenic animals 「New Developments in Stem Cell Research」(Editor : Erik V.Grier) (2007)
  • Author(s): Ogawa R, Fujimura J, Mizuno H, Hyakusoku H and Shimada T
  • Total Pages: 149-172
  • Publisher: NOVA Science publishers, New York(分担)