Loss of heterozygosity analysis and its relationship with Clinicopathological features in ameloblastoma

2008 Fiscal Year Final Research Report

• Project/Area Number: 19592109
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Okayama University
• Principal Investigator: NAGATSUKA Hitoshi Okayama University, 大学院・医歯薬学総合研究科, 准教授 (70237535)
• Co-Investigator(Kenkyū-buntansha): NAGAI Noriyuki 岡山大学, 名誉教授 (90085770) ; MEMETUTO Gundeuzu 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70333507) ; TUJIGIWA Hidetugu 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70335628) ; TAMAMURA Ryo 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00403494)
• Project Period (FY): 2007 – 2008
• Keywords: 歯原性腫瘍 / エナメル上皮腫 / 遺伝子解析 / ヘテロ接合性 / LOH解析癌抑制遺伝子 / INGファミリー

Research Abstract

腫瘍の発生には癌関連遺伝子の遺伝的変化が重要であることが知られている。本研究では、代表的な歯原性腫瘍であり、いまだ決定的治療法のないエナメル上皮腫について、腫瘍特異的な癌抑制遺伝子の同定と解析を目的とし、ヘテロ接合性消失解析による広範な遺伝子レベルでの検索を行った。結果からは、エナメル上皮腫瘍の再発や顎骨侵襲性に関連する癌抑制遺伝子の存在が示唆され、同腫瘍の特異性を解明する一助となる成果が見られた。

Research Products

• [Journal Article] Frequent deletion of ING2 locus at 4q35. 1 associates with advanced tumor stage in head and neck squamous cell carcinoma (2009)
  • Author(s): Borkosky, SS, Gunduz, M, Nagatsuka, H, Beder, LB, Gunduz, E, AI, Sheikh, Ali, M, Rodriguez, AP, Cilek, MZ, Tominaga, S, Yamanaka, N, Shimizu, K, Nagai, N
  • Journal Title: J Cancer Res Clin Oncol 135巻5号 Pages: 703-713
  • Peer Reviewed
• [Journal Article] Fine deletion analysis of 1p36 chromosomal region in oral squamous cell carcinomas (2008)
  • Author(s): Lefeuvre M, Gunduz M, Nagatsuka H, Gunduz E, Al Sheikh Ali M, Beder L, Fukushima K, Yamanaka N, Shimizu K, Nagai N
  • Journal Title: J Oral Pathol Med 38巻1号 Pages: 94-98
  • Peer Reviewed
• [Journal Article] Deletion at Dickkopf (dkk)-3 locus (11p15. 2) is related with lower lymph node metastasis and better prognosis in head and neck squamous cell carcinomas (2008)
  • Author(s): Katase N, Gunduz M, Beder L, Gunduz E, Lefeuvre M, Hatipoglu OF, Borkosky SS, Tamamura R, Tominaga S, Yamanaka N, Shimizu K, Nagai N, Nagatsuka H
  • Journal Title: Oncol Res 17巻6号 Pages: 273-282
  • Peer Reviewed
• [Journal Article] Sequential expressions of Notchl, Jagged2 and Mathl in molar tooth germ of mouse (2008)
  • Author(s): Borkosky SS, Nagatsuka H, Orita Y, Tsujigiwa H, Yoshinobu J, Gunduz M, Rodriguez AP, Missana LR, Nishizaki K, Nagai N,
  • Journal Title: Biocell 32巻3号 Pages: 251-258
  • Peer Reviewed
• [Journal Article] Lack of B-RAF mutations in head and neck squamous cell carcinoma (2008)
  • Author(s): Al Sheikh Ali M, Gunduz M, Gunduz E, Tamamura R, Beder L, Tominaga S, Onoda T, Yamanaka N, Grenman R, Shimizu K, Nagai N, Nagatsuka H
  • Journal Title: Folia Biol (Praha) 54巻5号 Pages: 157-161
  • Peer Reviewed
• [Journal Article] Notch signaling in benign and malignant ameloblastic neoplasms (2008)
  • Author(s): Nakano K, Siar CH, Tsujigiwa H, Nagatsuka H, Nagai N, Kawakami T
  • Journal Title: Eur J Med Res 13巻10号 Pages: 476-480
  • Peer Reviewed
• [Journal Article] Stromal cells promote bone invasion by suppressing bone formation in ameloblastoma (2008)
  • Author(s): Sathi GSA, Nagatsuka H, Tamamura R, Fujii M, Gunduz M, Inoue M, Rivera RS, Nagai N
  • Journal Title: Histopathology 53巻 Pages: 458-467
  • Peer Reviewed
• [Journal Article] Expression and mutation analysis of epidermal growth factor receptor in head and neck squamous cell carcinoma (2008)
  • Author(s): Sheikh Ali MA, Gunduz M, Nagatsuka H, Gunduz E, Cengiz B, Fukushima K, Beder LB, Demircan K, Fujii M, Yamanaka N, Shimizu K, Grenman R, Nagai N
  • Journal Title: Cancer Sci 99巻8号 Pages: 1589-1594
  • Peer Reviewed
• [Journal Article] The inhibitor of growth (ING) gene family : potential role in cancer therapy (2008)
  • Author(s): Gunduz M, Gunduz E, Rivera RS, Nagatsuka H
  • Journal Title: Curr Cancer Drug Targets 8巻4号 Pages: 275-284
  • Peer Reviewed
• [Journal Article] Downregulation of ING3 mRNA expression predicts poor prognosis in head and neck cancer (2008)
  • Author(s): Gunduz M, Beder LB, Gunduz E, NagatsukaH, Fukushima K, Pehlivan D, Cetin E, Yamanaka N, Nishizaki K, Shimizu K, Nagai N
  • Journal Title: Cancer Sci 99巻3号 Pages: 531-538
  • Peer Reviewed
• [Journal Article] Deletion mapping of chromosome 4q22-35 and identification of four frequently deleted regions in head and neck cancers (2008)
  • Author(s): Cetin E, Cengiz B, Gunduz E, Gunduz M, Nagatsuka H, Bekir-Beder L, Fukushima K, Pehlivan D, N MO, Nishizaki K, Shimizu K, Nagai N
  • Journal Title: Neoplasma 55巻4号 Pages: 299-304
  • Peer Reviewed
• [Journal Article] Involvement of EphA2 in head and neck squamous cell carcinoma : mRNA expression, loss of heterozygosity and immunohistochemical studies (2008)
  • Author(s): Rivera RS, Gunduz M, Nagatsuka H, Gunduz E, Cengiz B, Fukushima K, Beder LB, Pehlivan D, Yamanaka N, Shimizu K, Nagai N
  • Journal Title: Oncol Rep 19巻5号 Pages: 1079-1084
  • Peer Reviewed
• [Journal Article] Inhibitor of growth (ING) family : An emerging molecular target for cancer therapy (2008)
  • Author(s): Gunduz E, Gunduz M, Beder LB, Tamamura R, Nagatsuka H, Nagai N
  • Journal Title: Journal of Hard Tissue Biology 17巻1号 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Fine deletion mapping of chromosome 2q21-37 shows three preferentially deleted regions in oral cancer (2007)
  • Author(s): Cengiz B, Gunduz M, Nagatsuka H, Beder L, Gunduz E, Tamamura R, Mahmut N, Fukushima K, Ali MA, Naomoto Y, Shimizu K, Nagai N
  • Journal Title: Oral Oncol 43巻3号 Pages: 241-247
  • Peer Reviewed
• [Presentation] Examination of loss of heterozygosity in 1p36 chromosomal region in oral squamous cell carcinoma (2008)
  • Author(s): Lefeuvre Mathieu
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] Microsatellite analysis of ING tumor suppressor genes in ameloblastoma (2008)
  • Author(s): Silvia Susana Borkosky
  • Organizer: 第17回硬組織再生生物学会
  • Place of Presentation: 徳島
  • Year and Date: 2008-08-30
• [Presentation] Stromal Cells Promote Bone Invasion by Suppressing BoneFormation in Ameloblastoma (2008)
  • Author(s): Gul San Ara Sathi
  • Organizer: 第17回硬組織再生生物学会
  • Place of Presentation: 徳島
  • Year and Date: 2008-08-30
• [Presentation] Expression and mutation analysis of epidermal growth factor receptor (EGER) in head and neck squamouscellcarcinoma (2008)
  • Author(s): Mahmoud Al Sheikh Ali
  • Organizer: 第19回日本臨床口腔病理学会
  • Place of Presentation: 東京
  • Year and Date: 2008-08-20
• [Presentation] 頭頸部扁平上皮癌におけるDkk遺伝子ファミリーのLOH解析 (2008)
  • Author(s): 片瀬直樹
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Presentation] エナメル上皮腫問質細胞における骨吸収因子の免疫組織化学的検討 (2008)
  • Author(s): 玉村亮
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Presentation] 頭頸部扁平上皮癌における転移抑制遺伝子候補遺伝子に関する研究 (2008)
  • Author(s): Beder, Levent
  • Organizer: 第26回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 大阪
  • Year and Date: 2008-02-21
• [Presentation] Molecular Analysis of ING3 andING4 and Their Prognostic Role in HeadandNeck Cancers (2007)
  • Author(s): Gunduz, E
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] 頭頸部癌における癌抑制遺伝子ファミリー新規メンバーING3及びING4の分子生物学的解析 (2007)
  • Author(s): グンデゥズエスラ
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌
  • Year and Date: 2007-08-31
• [Presentation] 頭頸部癌におけるEGFRシグナル経路の分子生物学及び組織病理的解析 (2007)
  • Author(s): グンデゥズメーメット
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌
  • Year and Date: 2007-08-31
• [Presentation] Down-regulation of ING3 mRNA Expression Predicts Poor Prognosis inHeadandNeckcancer (2007)
  • Author(s): Beder, Levent
  • Organizer: 第31回日本頭頸部癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-06-13
• [Presentation] Tumor suppressor genes, Oncogenes and gene therapy in cancer (2007)
  • Author(s): Gunduz, Mehmet
  • Organizer: 2nd Agean Pharmacogenomics meeting
  • Place of Presentation: Isparta, Turkey
  • Year and Date: 2007-05-25
• [Presentation] Single nucleotide polymorphisms and individualized therapy (2007)
  • Author(s): Gunduz, Mehmet
  • Organizer: 2nd Agean Pharmacogenomics meeting
  • Place of Presentation: Isparta, Turkey
  • Year and Date: 2007-05-25
• [Presentation] The Role of EGFR Pathway in Head and Neck Squamous Cell Carcinomas (2007)
  • Author(s): Gunduz, M
  • Organizer: 第98回AACR
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2007-04-14
• [Presentation] Molecular analysis of ING4 and its splicing variants in oral cancer (2007)
  • Author(s): Gunduz, E
  • Organizer: 第98回AACR
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2007-04-14