Cell dynamics in transition stage from crown to tooth root formation and effects of growth factors in development of Hertwig epithelial root sheath

2008 Fiscal Year Final Research Report

• Project/Area Number: 19592128
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: FUJIWARA Naoki Iwate Medical University, 歯学部, 講師 (20190100)
• Co-Investigator(Renkei-kenkyūsha): HARADA Hidemitsu 岩手医科大学, 歯学部, 教授 (70271210) ; OHSHIMA Hayato 新潟大学, 医歯学系, 教授 (70251824) ; ISHIZEKI Kiyoto 岩手医科大学, 歯学部, 准教授 (50057775) ; KAGIYA Tadayoshi 岩手医科大学, 歯学部, 助教 (30405774)
• Project Period (FY): 2007 – 2008
• Keywords: 歯の発生 / 歯根形成 / ヘルトヴィッヒ上皮鞘 / 器官培養 / EGF

Research Abstract

臼歯の歯根形成にはヘルトヴィッヒ上皮鞘(HERS)が重要な役割を果たすことが知られているが、その中で理解が乏しいHERS発達のメカニズムについて今回検討した。我々は2つのオリジナルな器官培養系を用いて上皮成長因子epidermal growth factor、インスリン様成長因子insulin-like growth factor-Iが、それぞれ歯冠形成からHERS形成の移行時期、HERSの伸長・発達に重要な作用を示すことを明らかにした。

Research Products

• [Journal Article] MAPK mediates Hsp25 signaling in incisor development (2009)
  • Author(s): Lee, MJ, Cai, J, Kwak, SW, Cho, SW, Harada, H, Jung, HS.
  • Journal Title: Histochem Cell Biol 19(e-Pub)
  • Peer Reviewed
• [Journal Article] Egf signaling regulates transition from crown to root formation in the development of mouse molars (2008)
  • Author(s): Fujiwara, N., Akimoto, T., Kagiya, T., Ishizeki, K., Harada, H.
  • Journal Title: Journal of Experimental Zoology-Molecular and Developmental Evolution, Dec. 17(e-Pub)
  • Peer Reviewed
• [Journal Article] Molecular mechanisms regulating transition from crown to root formation in the development of mouse molars (2008)
  • Author(s): Fujiwara, N., Kagiya, T., Ishizeki, K., Harada, H.
  • Journal Title: J Oral Bioscience 50 Pages: 154-159
  • Peer Reviewed
• [Journal Article] The effects of LAMP1 and LAMP3 on M180 amelogenin uptake, localization and amelogenin mRNA induction by amelogenin protein (2008)
  • Author(s): Xu L, Harada H, Taniguchi A.
  • Journal Title: J. Biochem 144(4)(Epub) Pages: 531-537
  • Peer Reviewed
• [Journal Article] BMP2-induced gene profiling in dental epithelial cell line (2008)
  • Author(s): Miyoshi, K., Nagata, H., Horiguchi, T., Abe, K., Wahyudi, I.A., Baba, Y., Harada H., Noma, T.
  • Journal Title: J. Med. Invest 55 Pages: 216-226
  • Peer Reviewed
• [Journal Article] The epithelial- mesenchymal interaction plays a role in the maintenance of the stem cell niche of mouse incisors via Fgf10 and Fgf9 signaling. (2008)
  • Author(s): Yokohama-Tamaki, T., Fujiwara, N., Shibata, S., Wakisaka, S. and Harada, H.
  • Journal Title: Open Biotechnol J. 2 Pages: 111-115
  • Peer Reviewed
• [Journal Article] 歯冠形成から歯根形成に移行するメカニズム (2007)
  • Author(s): 原田英光、藤原尚樹、大島勇人
  • Journal Title: 岩医大歯誌 32 Pages: 97-104
  • Peer Reviewed
• [Journal Article] Establishment of immortalized dental follicle cells for generating periodontal ligament in vivo (2007)
  • Author(s): Yokoi, T., Saito, M., Kiyono, T., Iseki, S., kosaka,K., Nishida, E., Tsubakimoto, T., Harada, H., Eto, K., Noguchi, T. and Teranaka, T.
  • Journal Title: Cell Tissue Res. 327(2) Pages: 301-11
  • Peer Reviewed
• [Journal Article] Effect of BMP-2 on gene expression of enamel matrix proteins s at the dental epithelial cell line (2007)
  • Author(s): Xu, L., Takahashi, R., Harada, H. and Taniguchi, A.
  • Journal Title: Open Biotechnol J. 1 Pages: 18-20
  • Peer Reviewed
• [Journal Article] Egf prevents formation of Hertwig's epithelial root sheath during developing mouse molar tooth in vitro (2007)
  • Author(s): Fujiwara, N., Kagiya, T., Ishizeki, K. and Harada, H.
  • Journal Title: Eur Cell Mat. 14(Proceeding Suppl. 2) Pages: 54
• [Journal Article] A role of Wnt5a in continuously growing mice incisors (2007)
  • Author(s): Kagiya, T., Fujiwara, N., Ishizeki, K. Xiao, J. and Harada, H.
  • Journal Title: Eur Cell Mat. 14, Suppl Proceeding 2 p91
• [Presentation] 歯胚組織中の細胞動態を観察するためのimaging systemの開発 (2009)
  • Author(s): 藤原尚樹、大津圭史、秋元義、鍵谷忠慶、石関清人、原田英光
  • Organizer: 第114回日本解剖学会学術集会
  • Place of Presentation: 岡山理科大学(岡山)
  • Year and Date: 2009-03-30
• [Presentation] 歯の再生を目的としたiPS細胞の上皮、間葉細胞への分化誘導と分離培養 (2009)
  • Author(s): 大津圭史、藤原尚樹、石関清人、鍵谷忠慶、原田英光
  • Organizer: 第114回日本解剖学会学術集会
  • Place of Presentation: 岡山理科大学(岡山)
  • Year and Date: 2009-03-30
• [Presentation] Hertwig's epithelial root sheath is made of outer enamel epithelium (2008)
  • Author(s): Fujiwara, N., Kagiya, T., Ishizeki, K., Otsu, K., Akimoto and T., Harada, H.
  • Organizer: Gordon Research Conference on Craniofacial Morphogenesis & Tissue Regeneration
  • Place of Presentation: Ciocco, Barga, Italy
  • Year and Date: 20080211-15
• [Presentation] 歯冠から歯根形成への移行過程におけるEGFsignalingの役割 (2008)
  • Author(s): 藤原尚樹、大津圭史、秋元義、鍵谷忠慶、石関清人、原田英光
  • Organizer: 第50回歯科基礎医学会学術大会
  • Place of Presentation: 昭和大学(東京)
  • Year and Date: 2008-09-23
• [Presentation] マウス切歯の組織幹細胞を用いた歯の再生. シンポジウムS28細胞分化と口腔領域における再生 (2008)
  • Author(s): 原田英光、藤原尚樹、鍵谷忠慶、石関清人
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-27
• [Presentation] ヘルトヴィッヒ上皮鞘細胞の不死化 (2008)
  • Author(s): 秋元義、藤原尚樹、鍵谷忠慶、石関清人、原田英光
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-27
• [Presentation] マウス切歯の組織幹細胞を用いた歯の再生. シンポジウム20歯の再生研究の最前線 (2008)
  • Author(s): 原田英光、藤原尚樹、鍵谷忠慶、石関清人
  • Organizer: 第7回日本再生医療学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-13
• [Presentation] 歯の幹細胞の維持機構の解明から歯の再生への展開 (2007)
  • Author(s): 原田英光、藤原尚樹、鍵谷忠慶、石関清人
  • Organizer: ワークショップ5W6、歯の発生・再生の分子メカニズム、第30回日本分子生物学会年会と第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-15
• [Presentation] Mechanisms on maintenance of dental stem cells and how to make a biotooth (2007)
  • Author(s): Harada, H., Fujiwara, N., Yokohama-Tamaki, T., Kagiya, T., Tabata, Y. and Ishizeki. K.
  • Organizer: 1^<st> Asian Biomaterials Congress(Integrated Congress of 6^<th> Asian International Symposium on Biomaterials and 8^<th> Asian Symposium on Biomedical Materials)
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2007-12-08
• [Presentation] EGF prevent formation of Hertwig's epithelial root sheath during developing mouse molar tooth in vitro (2007)
  • Author(s): Fujiwara, N., Kagiya, T., Ishizeki, K. and Harada, H.
  • Organizer: 9th International Conference on Tooth Morphogenesis and Differentiation 2007
  • Place of Presentation: Zurich, Switzerland
  • Year and Date: 2007-09-08
• [Presentation] A role of Wnt5a in continuously growing mice incisors (2007)
  • Author(s): Kagiya, T., Fujiwara, N., Ishizeki, K., Xiao, J. and Harada, H.
  • Organizer: 9th International Conference on Tooth Morphogenesis and Differentiation 2007
  • Place of Presentation: Zurich, Switzerland
  • Year and Date: 2007-09-06
• [Presentation] Fgf-9 play a role for the maintenance of stem cell niche via Fgf-10 expression in the mouse incisors (2007)
  • Author(s): Yokohama-Tamaki, T., Shibata, S., Wakisaka, S. and Harada, H.
  • Organizer: 9th International Conference on Tooth Morphogenesis and Differentiation
  • Place of Presentation: Zurich, Switzerland
  • Year and Date: 2007-09-06
• [Presentation] 器官培養を用いた歯根発生メカニズムの解明. サテライトシンポジウムSS5-5、ヘルトヴィッヒ上皮鞘(HERS)と歯根発生のバイオロジー (2007)
  • Author(s): 藤原尚樹
  • Organizer: 第49回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2007-08-29
• [Presentation] ヘルトビッヒの上皮鞘(HERS)形成過程の新規仮説と歯根発生メカニズム. サテライトシンポジウムSS5-5、ヘルトヴィッヒ上皮鞘(HERS)と歯根発生のバイオロジー (2007)
  • Author(s): 原田英光, 鍵谷忠慶, 藤原尚樹, 石関清人
  • Organizer: 第49回歯科基礎医学会学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2007-08-29
• [Presentation] 幹細胞を用いた歯科再生研究の現状と臨床への展望と課題 (2007)
  • Author(s): 原田英光
  • Organizer: 第24回日本顎顔面補綴学会総会
  • Place of Presentation: 盛岡(特別講演)
  • Year and Date: 2007-07-19
• [Book] 口腔・顔面と歯・唾液腺・舌の発生(口腔内化学, 尾崎登喜雄編集・監修) (2008)
  • Author(s): 原田英光
  • Total Pages: 27-34
  • Publisher: 飛鳥出版
• [Book] 器官培養系を用いた歯根形成メカニズムの解明歯界展望(111(5)) (2008)
  • Author(s): 藤原尚樹
  • Total Pages: 957
  • Publisher: 医歯薬出版
• [Book] 歯冠形成から歯根形成に移行するメカニズム歯界展望(111(5)) (2008)
  • Author(s): 原田英光
  • Total Pages: 961
  • Publisher: 医歯薬出版