Identification of phosphorylated proteins in murine B cells in relation to the LPS-induced proliferative response

2008 Fiscal Year Final Research Report

• Project/Area Number: 19592129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: KIMURA Shigenobu Iwate Medical University, 歯学部, 教授 (10177917)
• Co-Investigator(Kenkyū-buntansha): OHARA-NEMOTO Yuko 長崎大学大学院, 医歯薬学総合研究科, 准教授 (10164667)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Minoru 岩手医科大学, 歯学部, 准教授 (40187133) ; TAJIKA Shihoko 岩手医科大学, 歯学部, 講師 (20048531) ; SHIMOYAMA Yu 岩手医科大学, 歯学部, 助教 (90453331)
• Project Period (FY): 2007 – 2008
• Keywords: LPS / B細胞 / 細胞内リン酸化反応

Research Abstract

LPSによるB細胞の増殖活性にかかわる細胞内シグナル伝達系を明らかにする目的で, LPS刺激後のB細胞の増殖反応と細胞内リン酸化反応について検討を行った結果, 増殖反応がみられた場合には25kDaの基質タンパク質(p25)のチロシンリン酸化反応が誘導された. そこで, p25の性状解析を行った結果, p25はRanであること, また無刺激でも複数のp25のスポットが観察されることが明らかとなった. さらに, LPS刺激によりp25のチロシンリン酸化総量が増加し, 且つその最も酸性側のスポットの量が増加することが明らかとなった.

Research Products

• [Journal Article] Gene expression analyses of human macrophage phagocytizing sub-m titanium particles by allergy DNA chip (GenopalTM) (2009)
  • Author(s): Taira, M., Nezu, T., Sasaki, M., Kimura, S., Kagiya, T., Harada, H., Narushima T. and Araki, Y.
  • Journal Title: Biomed. Mater. Eng. 19(in press)
• [Journal Article] Effects of fosfomycin on Shiga toxin-producing Escherichia coli : quantification of copy numbers of Shiga toxin genes and their expression levels using real-time PCR (2009)
  • Author(s): Ichinohe, N., Ohara-Nemoto, Y., Nemoto, T. K., Kimura, S. and Ichinohe, S.
  • Journal Title: J. Med. Microbiol. (in peress)
• [Journal Article] Determination of three amino acids that caused the alteration of proteolytic activities of staphylococcal glutamyl endopeptidases (2009)
  • Author(s): Nemoto, T. K., Ono, T., Shimoyama, Y., Kimura, S. and Ohara-Nemoto, Y.
  • Journal Title: Biol. Chem 390 Pages: 277-285
• [Journal Article] Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli (2008)
  • Author(s): Nemoto, T. K., Ohara-Nemoto, Y., Ono, T., Kobayakawa, T., Shimoyama, Y., Kimura, S. and Takagi, T.
  • Journal Title: FEBS J. 275 Pages: 573-587
• [Journal Article] An Escherichia coli expression system for glutamyl endopeptidases optimized by complete suppression of auto-degradation (2008)
  • Author(s): Nemoto, T.K., Ono, T., Shimoyama, Y., Kimura, S. and Ohara-Nemoto, Y.
  • Journal Title: Anal. Biochem 381 Pages: 74-80
• [Journal Article] Homologous and heterologous expression and maturation processing of extracellular glutamyl endopeptidase of Staphylococcus epidermidis (2008)
  • Author(s): Ohara-Nemoto, Y., Ono, T., Shimoyama, Y., Kimura, S. and Nemoto, T.K.
  • Journal Title: Biol. Chem. 389 Pages: 1209-1217
• [Journal Article] TEM/EDX observation of micro-beads of magnetically activated cell sorting (MACS) system and separation trial of mouse mesenchymal stem cells from bone marrow cells by MACS system (2008)
  • Author(s): Taira, M., Sasaki, K., Saitoh, S., Nezu, T., Kagiya, T., Harada, H., Sasaki, M., Kimura, S., Hirata, I. and Araki, Y.
  • Journal Title: J. Oral Tissue Engin. 6 Pages: 33-40
• [Journal Article] Occurrence of staphylococci in the oral cavities of healthy adults and nasal-oral trafficking of the bacteria (2008)
  • Author(s): Ohara-Nemoto, Y., Haraga, H., Kimura, S. and Nemoto, T.K.
  • Journal Title: J. Med. Microbiol 57 Pages: 95-99
• [Journal Article] Dose-dependent effects of Ni(II)ions on production of three inflammatory cytokines(TNF-・, IL-1・ and IL-6), superoxide dismutase(SOD)and free radical NO by murine macrophage-like RAW264 cells with or without LPS-stimulation (2008)
  • Author(s): Taira, M., Sasaki,M., Kimura, S. and Araki, Y.
  • Journal Title: J. Mater. Sci : Mater. Med. 192008.(EPub 2007, Nov 28) Pages: 2173-2178
• [Presentation] Rapid identification of HACEK group bacteria using 16S rRNA gene PCR-RFLP (2009)
  • Author(s): Sasaki, M., Tajika, S., Kodama, S., Shimoyama, Y. and Kimura, S.
  • Organizer: The 3rd International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai. Japan
  • Year and Date: 2009-01-16
• [Presentation] A novel aspartate-specific dipeptidylpeptidase produced from Porphyromonas endodontalis (2009)
  • Author(s): Haraga, H., Ohara-Nemoto, Y., Nemoto, T. K., Shimoyama, Y., Sasaki, M. and Kimura, S.
  • Organizer: The 3rd International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai. Japan
  • Year and Date: 2009-01-16
• [Presentation] The production of secretory leukocyte protease inhibitor(SLPI)from gingival epithelial cells in response to Porphyromounas gingivalis lipopoly-saccharides (2009)
  • Author(s): Ishikawa, T., Ohara-Nemoto, Y., Tajika, S., Sasaki, M. and Kimura, S.
  • Organizer: The 3rd International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai. Japan
  • Year and Date: 2009-01-16
• [Presentation] Three amino acid substitutions are sufficient for the alteration of the activities among three members of Staphylococcal glutamic-acid-specific endopeptidase (2008)
  • Author(s): Nemoto, T. K., Ono, T., Shimoyama, Y., Kimura, S. and Ohara-Nemoto, Y.
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Effects of high-concentration ID10% copper ions on macrophage (2008)
  • Author(s): Taira, M., Kagiya, T., Harada, H., Sasaki, M., Kimura, S. and Araki, Y.
  • Organizer: Annual Meeting 2008 of the Academy of Dental Materials
  • Place of Presentation: Wurzburg, Germany
  • Year and Date: 2008-10-02
• [Presentation] Gene expression analyses of human macrophage phagocytizing sub-μtitanium particles by allergy DNA chip (Genopal^<TM>) (2008)
  • Author(s): Taira, M., Nezu, T., Sasaki, M., Kimura, S., Kagiya, T., Harada, H., Narushima, T. and Araki, Y.
  • Organizer: International Symposium on Nanotoxi- cology Assessment and Biomedical, Environmental Application of Fine Particles and Nanotubes ISNT2008
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2008-06-16
• [Presentation] Maturation processing of glutamyl endopeptidase of Staphylococcus epidermidis (2008)
  • Author(s): Ohara-Nemoto, Y., Ono, T., Shimoyama, Y., Kimura, S. and Nemoto, T.K.
  • Organizer: 108th General Meeting of the American Society for Microbiology
  • Place of Presentation: Boston, USA.
  • Year and Date: 2008-06-05
• [Presentation] Acid tolerance of Steptococcus anginosus (2008)
  • Author(s): Sasaki, M., Tajika, S., Kodama, S. and Kimura, S.
  • Organizer: 18th European Congress of Clinical Microbiology
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2008-04-20
• [Presentation] Macrophage reaction against sub-m titanium particle (2007)
  • Author(s): Taira, M., Sasaki, M., Kimura, S. and Araki, Y.
  • Organizer: Annual Meeting of the Academy of Dental Materials 2007
  • Place of Presentation: Fort Lauderdale, FL, USA.
  • Year and Date: 2007-10-23
• [Presentation] Pathogenic factors of Porphyromonas endodontalis (2007)
  • Author(s): Haraga, H., Ohara-Nemoto, Y., Nemoto, T.K., Sasaki, M., Tajika, S., Hamada, S. and Kimura, S.
  • Organizer: 107th General Meeting of the American Society for Microbiology
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2007-03-22
• [Book] 基礎-I-1. 口腔臨床に必要な細菌学 (2008)
  • Author(s): 木村重信, 浜田茂幸
  • Total Pages: 1-10
  • Publisher: In口腔内科学(尾崎登喜雄), 飛鳥出版室, 高知
• [Book] Early childhood caries and childhood periodontal diseases (2007)
  • Author(s): Kimura, S., and Ohara-Nemoto, Y.
  • Total Pages: 177-197
  • Publisher: In Pediatric Infectious Diseases Revisited(H. Schroten, & S. Wirth, eds.), Birkhauser-Verlag AG, Basel.
• [Book] Proinflammatory cytokine production and leukocyte adhesion molecule expression of endothelial cells in response to Abiotrophia defectiva infection (2007)
  • Author(s): Tajika, S., Sasaki, M., Agato, S., Harada-Oikawa, R., Hamada, S. and Kimura, S.
  • Total Pages: 285-286
  • Publisher: In Interface Oral Health Science 2007(M. Watanabe, N. Takahashi, & H. Takada, eds.), Springer Japan, Tokyo.
• [Book] Genome-wide gene expression analysis of human myelomonocytic cell line THP-1 exposed to lipopolysaccharide(LPS) (2007)
  • Author(s): Taira, M., Sasaki, M., Kimura, S. and Aaraki, Y.
  • Total Pages: 293-294
  • Publisher: In Interface Oral Health Science 2007(M. Watanabe, N. Takahashi, & H. Takada, eds.), Springer Japan, Tokyo.
• [Book] CD14-dependent and independent B-cell activations by stimulation with lipopolysaccharide from Porphyromonas gingivalis (2007)
  • Author(s): Shimoyama, Y., Ohara-Nemoto, Y., Yamada, A., Kato, H., Tajika, S. and Kimura, S.
  • Total Pages: 295-296
  • Publisher: In Interface Oral Health Science 2007(M. Watanabe, N. Takahashi, & H. Takada, eds.), Springer Japan, Tokyo.