Proteomics analysis of bacterial interaction in biofilm

2008 Fiscal Year Final Research Report

• Project/Area Number: 19592132
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: ISHIHARA Kazuyuki Tokyo Dental College, 教授 (00212910)
• Co-Investigator(Kenkyū-buntansha): INOUE Takashi 東京歯科大学, 教授 (20125008) ; KIMIZUKA Ryuta 東京歯科大学, 講師 (90287178) ; YAMANAKA Ayumi 東京歯科大学, 助教 (40231667)
• Project Period (FY): 2007 – 2008
• Keywords: Biofilm / gene expression / Porphyromonas gingivalis / Fusobacterium nucleatum

Research Abstract

難治性根尖性歯周組織炎患者から菌を分離し、それらの菌種間のバイオフィルム形成における相互作用について検討を加えた。得られた菌株のうち複数の菌種間でバイオフィルム形成における相乗効果が認められた。これらのうちその効果が最も顕著に認められたFusobacteriumnucleatumとPorphyromonas gingivalis間で相互作用の解析の結果、P. gingivalisからの可用性物質によってF. nucleatumのバイオフィルム形成が促進され、F. nucleatum菌体のタンパクプロファイルに変化していた。さらにこの組み合わせでの混合感染によって、歯肉上皮細胞および血管内皮細胞へのP. gingivalis侵入効率の上昇が認められ、複数の細菌によるpolymicrobialinfectionによる病原性の変化が明らかになった。

Research Products

• [Journal Article] Characterization of bacterial flora in persistent apical periodontitis lesions (2009)
  • Author(s): Fujii, R., Saito, Y., Tokura, Y., Nakagawa, K.-I., Okuda, K. and Ishihara, K.
  • Journal Title: Oral Microbio.1 Immunol. (in press)
  • Peer Reviewed
• [Journal Article] Analysis of Matrix Metalloproteinase (MMP-8 and MMP-2) Activity in Gingival Crevicular Fluid from Children with Down's Syndrome (2009)
  • Author(s): Yamazaki-Kubota, T., Miyamoto, M., Sano, Y., Kusumoto, M., Yonezu, T., Sugita, K., Okuda, K., Yakusiji, M. and Ishihara, K.
  • Journal Title: J. Periodont. Res. (in press)
  • Peer Reviewed
• [Journal Article] Tongue-coating as risk indicator for aspiration pneumonia in edentate elderly (2008)
  • Author(s): Abe, S., Ishihara, K., Adachi, M. and Okuda, K.
  • Journal Title: Arch. Gerontol. Geriatr. 47 Pages: 267-275
  • Peer Reviewed
• [Journal Article] Colonization pattern of periodontal bacteria in Japanese children and their mothers (2008)
  • Author(s): Kobayashi, N., Ishihara, K., Sugihara, N., Kusumoto, M., Yakushiji, M. and Okuda, K.
  • Journal Title: J. Periodontal Res. 43 Pages: 156-161
  • Peer Reviewed
• [Journal Article] Fusobacterium nucleatum enhances invasion of human gingival epithelial and aortic endothelial cells by Porphyromonas gingivalis (2008)
  • Author(s): Saito, A., Inagaki, S., Kimizuka, R., Okuda, K., Hosaka, Y., Nakagawa, T. and Ishihara, K.
  • Journal Title: FEMS Immunol. Med. Microbiol. 54 Pages: 349-355
  • Peer Reviewed
• [Journal Article] Stimulation of Fusobacterium nucleatum biofilm formation by Porphyromonas gingivalis (2008)
  • Author(s): Saito, Y., Fujii, R., Nakagawa, K.-I., Okuda, K. and Ishihara, K.
  • Journal Title: Oral Microbiol. Immunol. 23 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Treponema denticola induces interleukin-8 and macrophage chemoattractant protein 1 production in human umbilical vein epithelial cells (2007)
  • Author(s): Okuda, T., Kimizuka, R., Miyamoto, M., Kato, T., Yamada, S., Okuda, K. and Ishihara, K.
  • Journal Title: Microb. Infect. 9 Pages: 907-913
  • Peer Reviewed
• [Journal Article] Inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of Porphyromonas gingivalis (2007)
  • Author(s): Yamanaka, A., Kouchi, T., Kasai, K., Kato, T., Ishihara, K. and Okuda, K.
  • Journal Title: J. Periodontal. Res. 42 Pages: 589-592
  • Peer Reviewed
• [Presentation] 歯周病原菌Treponema denticolaのヒト歯肉上皮細胞への侵入, 日本細菌学雑誌, 64, 164 (2009)
  • Author(s): 稲垣覚, 齋藤淳, 君塚隆太, 石原和幸
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 20090312-14
• [Presentation] Aggregatibacter actinomycetemcomitans rough型集落形成株のアミロイド形成, 日本細菌学雑誌, 64, 185 (2009)
  • Author(s): 君塚隆太, 加藤哲男, 岡田あゆみ, 石原和幸, 橋本貞充
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 20090312-14
• [Presentation] Treponema dinticola dentilisin involves to coaagregation with Porphyromonas gingivalis (2008)
  • Author(s): Sano, Y., Miyamato, M., Ito, R., Matsumoto, N., Yakushiji, M., Shintani, S. and Ishihara, K.
  • Organizer: Japanese Accociation for Dental Research
  • Place of Presentation: Nagoya
  • Year and Date: 20081129-30
• [Presentation] 歯周病原菌のPolymicrobial感染による宿主細胞への侵入解析, 日本歯周病学会会誌, 50, 120 (2008)
  • Author(s): 齋藤淳, 稲垣覚, 君塚隆太, 中川種昭, 山田了, 石原和幸
  • Organizer: 第51回秋期歯周病学会
  • Place of Presentation: 四日市
  • Year and Date: 20081019-20
• [Presentation] Rough 型Aggregatibacter actinomycetemcomitansはアミロイド様物質を形成する, Journal of Oral Biosciences, 50, 213 (2008)
  • Author(s): 君塚隆太, 加藤哲男, 岡田あゆみ, 橋本貞充, 石原和幸
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 20080923-25
• [Presentation] Fusobacterium nucleatum はPorphyromonas gingivalisの宿主細胞侵入を促進する, Journal of Oral Biosciences, 50, 115 (2008)
  • Author(s): 齋藤淳, 稲垣覚, 君塚隆, 石原和幸
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 20080923-25
• [Presentation] 歯周病の動脈硬化への関わり,日本心臓病学会誌, 2, 205 (2008)
  • Author(s): 石原和幸
  • Organizer: 第56回日本心臓病学会
  • Place of Presentation: 東京
  • Year and Date: 20080908-10
• [Presentation] Transmission of periodontopathic bacteria from natural tooth to implants, J. Dent. Res. (2008)
  • Author(s): Aoki, M., Ishihara, K., Takanashi, K., Honma, S., Ito, T., Yajima, K., Okuda, K. and Sato, T.
  • Organizer: 86th General Session & Exhibition of the International Association for dental Research
  • Place of Presentation: Tronto, Canada
  • Year and Date: 20080902-05
• [Presentation] Treponema denticola invasion of human gingival epithelial cells, J. Dent. Res. (2008)
  • Author(s): Inagaki, S., Kimizuka, R., Okuda, K. and Ishihara, K.
  • Organizer: 86th General Session & Exhibition of the International Association for dental Research
  • Place of Presentation: Tronto, Canada
  • Year and Date: 20080702-05
• [Presentation] Characterization of dentipain, a Streptococcus-pyogenes-Ides-protease homologue in Treponema denticola, J. Dent. Res. (2008)
  • Author(s): Ishihara, K., Wawrzonek, K., Shaw, L.N., Inagaki, S., Okuda, K. and Potempa, J.
  • Organizer: 86th General Session & Exhibition of the International Association for dental Research
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20080702-05
• [Presentation] HGP44 reduces Porphyromonas gingivalis induced alveolar bone loss in mice, J. Dent. Res. (2008)
  • Author(s): Muramatsu, K., Miyachi, K., Shibahara, T., Okuda, K. and Ishihara, K.
  • Organizer: 86th General Session & Exhibition of the International Association for dental Research
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20080702-05
• [Presentation] Aging affects oral microflora in japanese elderly, J. Dent. Res. (2008)
  • Author(s): Ryu, M., Ueda, T., Sakurai, K., Saito, T. and Ishihara, K.
  • Organizer: 86th General Session & Exhibition of the International Association for dental Research
  • Place of Presentation: Tronto, Canada
  • Year and Date: 20080702-05
• [Presentation] 歯周病原性菌プロテアーゼの病原性解析, 日本歯周病学会会誌, 50, 51 (2008)
  • Author(s): 石原和幸
  • Organizer: 第51回春期日本歯周病学会
  • Place of Presentation: 大宮
  • Year and Date: 20080424-25
• [Presentation] Hgp44 DNA vaccine によるPorphromonas gingivalis感染骨吸収の防御作用, 日本細菌学雑誌, 63, 158 (2008)
  • Author(s): 村松恭太郎, 片倉朗, 柴原孝彦, 奥田克爾, 石原和幸
  • Organizer: 第80回日本細菌学会
  • Place of Presentation: 京都
  • Year and Date: 20080324-26
• [Presentation] Treponema denticolaのbacterial immunity proteinの解析, 歯科学報, 108, 394 (2008)
  • Author(s): 田中公子, 伊藤理恵子, 新谷誠康, 藥師寺仁, 石原和幸
  • Organizer: 第286回東歯学会
  • Place of Presentation: 千葉
  • Year and Date: 20080324-26
• [Presentation] 無歯顎者の唾液中細菌叢に影響をおよぼす因子の探索, 老年歯科医学, 23, 156-157 (2008)
  • Author(s): 竜正大, 上田貴之, 櫻井薫, 安井雅子, 斎藤貴之, 石原和幸
  • Organizer: 19回老年歯科医学会総会
  • Place of Presentation: 岡山
  • Year and Date: 20080000
• [Presentation] Down症候群歯肉溝滲出液中MMPの検索, 障害者歯科, 28, 237 (2007)
  • Author(s): 久保田智子, 宮本暦, 米津卓郎, 佐野由美子, 奥田克爾, 石原和幸, 藥師寺仁
  • Organizer: 第24回日本障害者歯科学会
  • Place of Presentation: 長崎
  • Year and Date: 20071124-25
• [Presentation] Induction of Protective Immunity against Porphyromonas gingivalis Infection by rgpA DNA vaccine (2007)
  • Author(s): Ishihara, K.
  • Organizer: 55th Annual meeting of Japanese Association for Dental Research
  • Place of Presentation: Tsurumi, Japan
  • Year and Date: 20071117-18
• [Presentation] Treponema denticola 表層プロテアーゼdentilisin による病原性発現, 日本再生歯科医学会誌 (2007)
  • Author(s): 稲垣覚, 奥田克爾, 石原和幸
  • Organizer: 第5回日本再生歯科医学会
  • Place of Presentation: 東京
  • Year and Date: 20070922-23
• [Presentation] アンチエイジング治療と再生医療rgpA DNA vaccineによる歯周炎予防抗体の誘導, 日本再生歯科医学会誌 (2007)
  • Author(s): 石原和幸
  • Organizer: 第5回日本再生歯科医学会
  • Place of Presentation: 東京
  • Year and Date: 20070922-23
• [Presentation] 口腔細菌数に老化が与える影響, 日本再生歯科医学会誌, 5, 55 (2007)
  • Author(s): 竜正大, 上田貴之, 櫻井薫, 斎藤貴之, 石原和幸
  • Organizer: 第5回日本再生歯科医学会
  • Place of Presentation: 東京
  • Year and Date: 20070922-23
• [Presentation] Treponema denticolaは極性上皮細胞内に侵入する, Journal of Oral Biosciences, 49, 164 (2007)
  • Author(s): 君塚隆太, 石原和幸, 加藤哲男, 奥田克爾
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌
  • Year and Date: 20070830-31
• [Presentation] Hgp44 DNA vaccine による防御性抗体誘導, Journal of Oral Biosciences, 49, 130 (2007)
  • Author(s): 村松恭太郎, 片倉朗, 柴原孝彦, 奥田克爾,石原和幸
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌
  • Year and Date: 20070830-31
• [Presentation] 唾液中細菌数への唾液量と舌苔付着の影響, 老年歯科医学, 22, 184-185 (2007)
  • Author(s): 竜正大, 上田貴之, 櫻井薫, 斎藤貴之, 石原和幸
  • Organizer: 第18回老年歯科医学会総会
  • Place of Presentation: 札幌
  • Year and Date: 20070620-22
• [Presentation] Catecholamine increases RgpA expression in Porphyromonas gingivalis, J. Dent. Res.86 (2007)
  • Author(s): Saito, T., Sakurai, K., Okuda, K. and Ishihara, K.
  • Organizer: 85th General Session & Exhibition of the IADR/AADR/CADR
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20070321-24
• [Presentation] Treponema denticola 表層プロテアーゼdentilisin による病原性発現, 歯科学報, 107, 471 (2007)
  • Author(s): 稲垣覚, 奥田克爾, 石原和幸
  • Organizer: 第284回東歯学会総会
  • Place of Presentation: 千葉
  • Year and Date: 20070000
• [Presentation] 老化と口腔細菌数との関係, 歯科学報, 107, 471 (2007)
  • Author(s): 竜正大, 上田貴之, 櫻井薫, 斎藤貴之, 石原和幸
  • Organizer: 第284回東歯学会総会
  • Place of Presentation: 千葉
  • Year and Date: 20070000
• [Book] 口腔と全身疾患(中原泉, 鴨居久一編)
  • Author(s): 石原和幸, 著分担
  • Publisher: クインテッセンス出版, 東京