Study of mechanical tensile stress effects on the synthesis of osteoprotegerin in microvascular endothelium

2008 Fiscal Year Final Research Report

• Project/Area Number: 19592364
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: ISOGAI Emiko Health Sciences University of Hokkaido, 歯学部, 講師 (80113570)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Michiyo 北海道医療大学, 歯学部, 助手 (80316265) ; ARAKAWA Toshiya 北海道医療大学, 歯学部, 講師 (40306254)
• Project Period (FY): 2007 – 2008
• Keywords: オステオプロテゲリン / マトリックス / 血管内皮細胞

Research Abstract

矯正力により歯を移動させる際に、歯根膜に豊富に存在する微小血管は骨や歯根膜のリモデリングに重要な役割を果たす。我々は破骨細胞活性化抑制因子 (OPG)の血管内皮細胞に及ぼす作用と伸展刺激に伴うOPGの発現動態について検討を行った。OPGにより、血管新生能の亢進および血管内皮細細胞の著しい伸展や微小管の伸長が観察された。しかし、TNFα刺激下、血管内皮細胞において、24時間持続的伸展刺激によるOPG産生量の変化は認められなかった。本研究結果より、機械的刺激による歯周組織のOPG濃度の変化に応じて血管の再構築などに作用している可能性が示唆された。

Research Products

• [Journal Article] Antimicrobial activity of three tick defensins and four mammalian cathelicidin-derived synthetic peptides against Lyme disease spirochetes and bacteria isolated from the midgut (2009)
  • Author(s): Isogai, E., Isogai, H., Takahashi, K., Kobayashi-Sakamoto, M. & Okumura, K.
  • Journal Title: Exp Appl Acarol
  • Peer Reviewed
• [Journal Article] P. B. Ceragenin CSA-13 exhibits antimicrobial activity against cariogenic and periodontopathic bacteria. (2009)
  • Author(s): Isogai, E., Isogai, H., Takahashi, K., Okumura, K. & Savage
  • Journal Title: Oral Microbiol Immunol 24 Pages: 170-172
  • Peer Reviewed
• [Journal Article] Role of alphav integrin in osteoprotegerin-induced endothelial cell migration and proliferation (2008)
  • Author(s): Kobayashi-Sakamoto, M., Isogai, E., Hirose, K. & Chiba, I.
  • Journal Title: Microvasc Res 76 Pages: 139-144
  • Peer Reviewed
• [Journal Article] Negative regulation of quorum-sensing systems in Pseudomonas aeruginosa by ATP-dependent Lon protease (2008)
  • Author(s): Takaya, A., Tabuchi, F., Tsuchiya, H., Isogai, E. & Yamamoto, T
  • Journal Title: J Bacteriol 190 Pages: 4181-4188
  • Peer Reviewed
• [Journal Article] The role of streptococcal hypersensitivity in the pathogenesis of Behcet's Disease (2008)
  • Author(s): Kaneko, F., Oyama, N., Yanagihori, H., Isogai, E., et al
  • Journal Title: Eur J Dermatol 18 Pages: 489-498
  • Peer Reviewed
• [Journal Article] Effect of catechin diet on gingivitis in cats (2008)
  • Author(s): Isogai, H., Isogai, E., Takahashi, K. & Kurebayashi, Y
  • Journal Title: Int J Applres Vet M 6 Pages: 82-86
  • Peer Reviewed
• [Journal Article] Tick biology and tick-borne infections (2008)
  • Author(s): Isogai E, Isogai H, Hayashi S
  • Journal Title: Current Trend in Microbiology in press
  • Peer Reviewed
• [Journal Article] Profiling of differentially expressed genes in porcine epithelial cells derived from periodontal ligament and gingiva by DNA microarray (2008)
  • Author(s): Kurashige, Y., Saitoh, M., Nishimura, M., Noro, D., Kaku, T., Igarashi, S., Takuma, T., Arakawa, T., Inoue, T., and Abiko, Y
  • Journal Title: Arch Oral Biol 53 Pages: 473-442
  • Peer Reviewed
• [Journal Article] Two distinct pathways for cyclooxygenase-2 protein degradation (2008)
  • Author(s): Mbonye, U.R., Yuan, C., Harris, C.E., Sidhu, R.S., Song, I., Arakawa, T., and Smith, W.L
  • Journal Title: J Biol Chem 283 Pages: 8611-623
  • Peer Reviewed
• [Journal Article] 歯科病院環境の真菌学的検討-病院環境におけるStachybotrys chartarum, Aspergillus niger, Chaetomium funicolaの分離 (2007)
  • Author(s): 磯貝恵美子、小林美智代、奥村一彦、磯貝 浩、榑林陽一、林 俊治
  • Journal Title: 環境感染 22(4) Pages: 260-265
  • Peer Reviewed
• [Presentation] Osteoprotegerin-mediated cytoskeletal reorganization in microvascular endothelium (2009)
  • Author(s): Kobayashi-Sakamoto M, Isogai, E, Hirose, K, Reid P, Chiba I, and Holen I
  • Organizer: IADR/AADR/CADR 87th General Session and Exhibition
  • Place of Presentation: Miami,USA
  • Year and Date: 20090401-04
• [Presentation] Pathogenesis of skin lesions in Lyme disese (2008)
  • Author(s): Isogai E, Isogai H, Takahashi K, Kobayashi-Sakamoto M,
  • Organizer: 11^<th> International Conference on Lyme Borreliosis and other tick-borne diseases
  • Place of Presentation: Irvine, USA
  • Year and Date: 20081019-23
• [Presentation] Savage PBFunctional properties of cationic antimicrobial compounds on the neutrophils and endothelial cells (2008)
  • Author(s): Isogai E ,Isogai H, Okumura K, Kobayashi-Salkamoto M, Kaneko F, Ohgami K, Ohno S
  • Organizer: 13^<th> Int Conf for Behcet's disease
  • Place of Presentation: Klagenfult
  • Year and Date: 20080524-27
• [Presentation] Protective effects of antimicrobial steroid (CSA-13) against bacterial infection with ocular manifestations (2008)
  • Author(s): Isogai H, Isogai E
  • Organizer: 13^<th> Int Conf for Behcet's disease
  • Place of Presentation: Klagenfult
  • Year and Date: 20080524-27
• [Presentation] Osteoprotegerin promotes proliferation and migration of human microvascular endothelial cells (2008)
  • Author(s): 小林 美智代, 磯貝恵美子
  • Organizer: 第57回日本口腔衛生学会総会,
  • Place of Presentation: 大宮
  • Year and Date: 2008-10-03
• [Presentation] Expression of hCAP18 is resistant factor against hCAP18-induced apoptosis (2007)
  • Author(s): Okumura K, Sawada N, Muraoka.K, Isogai E, Hosokawa Y, Shibata T, Isogai H
  • Organizer: 42th annual meeting IADR, Sep
  • Place of Presentation: Greece, Thessaloniki
  • Year and Date: 20070924-1004
• [Presentation] Osteoprotegerin promotes integrin αvβ3-dependnent endothelial cell migration (2007)
  • Author(s): Kobayashi-Sakamoto M, Isogai E, Hirose K, Chiba I,
  • Place of Presentation: Glasgow, UK
  • Year and Date: 20070708-12
• [Presentation] Gingival damages and oxidative stress in canine periodontitis (2007)
  • Author(s): Isogai H, Isogai E
  • Organizer: ASM General 107^<th> Meeting, ASM General 107^<th> Meeting
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20070521-25