2010 Fiscal Year Final Research Report
Genetic study on dental attrition in mice
| Project/Area Number |
19592368
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
MAEDA Takahide Nihon University, 松戸歯学部, 教授 (70130599)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Kei 日本大学, 松戸歯学部, 助教 (20453889)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 咬耗 / 連鎖解析 / 候補遺伝子 |
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| Research Abstract |
The aim of this study was to identify the chromosomes harboring candidate causative gene(s) for dental attrition using pools of DNA from two strains mice with different susceptibilities to attrition. Attrition scores in F2 mice aged 260 days obtained by genetic crossing of C3H and B6 mice ranged from low to high. DNA from four F2 mice with high attrition scores and four F2 mice with low attrition scores was pooled. The 53 Mit markers used in this study were determined as informative markers, being polymorphisms between C3H and B6, and were distributed among all auto chromosomes in mice. This suggests that the candidate chromosomes harboring causative gene(s) for attrition are linked with D4Mit13 and D13Mit9, which were identified as homozygous alleles of C3H in Pool High DNA and homozygous alleles of B6 in Pool Low DNA.
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