Elucidation of mechanism of enhanced innate salivary-IgA antibody to Thymus-independent antigen

2008 Fiscal Year Final Research Report

• Project/Area Number: 19592403
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Social dentistry
• Research Institution: The University of Tokushima (2008); Osaka University (2007)
• Principal Investigator: KATAOKA Kosuke The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 准教授 (50283792)
• Co-Investigator(Kenkyū-buntansha): ITOH Hiro-0 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (40213079) ; FUKUI Makoto 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (50325289)
• Co-Investigator(Renkei-kenkyūsha): MGATA Hideki 大阪大学, 大学院・歯学研究科, 准教授 (50260641) ; KUBONIWA Masae 大阪大学, 大学院・歯学研究科, 助教 (00303983)
• Research Collaborator: FUJIHASHI Kohtaro 米国アラバマ大学バーミングハム校, ワクチンセンター, 教授
• Project Period (FY): 2007 – 2008
• Keywords: 予防歯学 / ワクチン / アジュバント / 粘膜免疫 / 自然免疫 / 唾液IgA抗体

Research Abstract

我々はこれまで、粘膜アジュバントコレラトキシン(CT)が粘膜部における胸腺非依存性抗原特異的抗体を誘導し、自然免疫システムにおいても粘膜アジュバントとしての活性能を有する可能性を示してきた。本研究では、胸腺非依存性抗原と共にCTをマウスに経鼻投与した場合、マウス唾液腺および鼻腔粘膜に誘導された樹状細胞とBIB細胞とのクロストークにより抗原特異的lgA抗体産生に関するクラススイッチングが誘導されることを認めた。このことは、CTが獲得免疫システムだけでなく自然免疫システムにおいてもアジュバント活性能を有する可能性を強く示唆するものである。

Research Products

• [Journal Article] Intranasal immunization with a mixture of PspA and a Toll-Like Receptor Agonist induces specific antibodies and enhances bacterial clearance in the airways of mice (2009)
  • Author(s): Oma K., Jizi Z., Ezoe H., Akeda Y., Koyama S., Ishii J. K., Kataoka K. and Oishi. K
  • Journal Title: Vaccine (in press)
  • Peer Reviewed
• [Journal Article] A combination of Flt3 Ligand cDNA and CpG ODN as nasal adjuvants elicits NALT dendritic cells for prolonged mucosal immunity (2008)
  • Author(s): Fukuiwa T., Sekine S., Kobayashi R., Suzuki H., Kataoka K., Gilbert R. S., Kurono Y., Boyaka P. N., Kreig A. M., McGhee J. R. and Fujihashi K
  • Journal Title: Vaccine 26(37) Pages: 4849-4859
  • Peer Reviewed
• [Journal Article] Association between involuntary smoking and salivary markers related to periodontitis (2008)
  • Author(s): Nishida N., Yamamoto Y., Tanaka M., Kataoka K., Kuboniwa M., Nakayama K., Morimoto K., Shizukuishi S
  • Journal Title: a two-year longitudinal study. jr. Periodontal 79(12) Pages: 2233-2240
  • Peer Reviewed
• [Journal Article] Ovalbumin-protein Ql M cell targeting facilitates oral tolerance with reduction of antigen-specific CD4+ T cells (2008)
  • Author(s): Suzuki H., Sekine S., Kataoka K., Pascual D., Maddaloni M., Kobayashi R., Fujihashi K., Kozono H., McGhee J. R. Fujihashi K
  • Journal Title: Gastroenterology 135(3) Pages: 917-925
  • Peer Reviewed
• [Journal Article] A novel adenovirus expressing Flt3 ligand enhances mucosal immunity by inducing mature NALT dendritic cell migration (2008)
  • Author(s): Sekine S., Kataoka K., Fukuyama Y., Adachi Y., Davydova J., Yamamoto M., Kobayashi R., Fujihashi K., Suzuki H., Curiel D., Shizukuishi. S., McGhee J., Fujihashi K
  • Journal Title: J. Immunol 180(12) Pages: 8126
  • Peer Reviewed
• [Journal Article] 「歯周病と喫煙との関連性についてのEBM」2008生命歯科医学のカッティング・エッジCuttint-Edge biodentistryn (2008)
  • Author(s): 雫石聰、田中宗雄、小島美樹、西田伸子、永田英樹、片岡宏介、久保庭雅恵
  • Journal Title: 大阪大学出版会 Pages: 109-119
• [Journal Article] 2008 Effect of eucalyptus extract chewing gum on periodontal health : Double-masked, randomized trial (2008)
  • Author(s): Nagata H., Inagaki Y., Tanaka M. Ojima M., Kataoka K., Kuboniwa M., Nishida N., Shimizu K., Osawa K., Shizukuisihi S
  • Journal Title: J. Periodontol 79(8) Pages: 1378-1385
  • Peer Reviewed
• [Journal Article] 2007Isolated lymphoid follicles are not IgA inductive sites for recombinant Salmonella (2007)
  • Author(s): Hashizume T., Momoi F., Kurita-Ochiai T., Kaminogawa S., Hosono A., Kataoka K., Kuwabara N., Kweon M.-N., Yamamoto M
  • Journal Title: Biochem. Biophys. Res. Commun 360(2) Pages: 388-393
  • Peer Reviewed
• [Journal Article] 特集I : IgAの産生をめぐつて「コレラトキシンの粘膜投与によるIgA産生B細胞の誘導」 (2007)
  • Author(s): 片岡宏介、雫石聰、藤橋浩太郎
  • Journal Title: 2007科学評論社臨床免疫・アレルギー科Clinical Immunology&Allergy 47(6) Pages: 640-646
• [Journal Article] Nasal cholera toxin elicits IL-5 and IL-5 receptor ox-chain expressing B-la B cells for innate mucosal IgA antibody responses (2007)
  • Author(s): Kataoka K., Fujihashi K., Sekine S., Fukuiwa T., Kobayashi R., Suzuki H., Nagata H., Takatsu K., Shizukuishi S., McGhee J. R., Fujihashi K
  • Journal Title: J. Immwiol 178(10) Pages: 6058-65
  • Peer Reviewed
• [Journal Article] Longitudinal study of the association between smoking as a periodontitis risk and salivary biomarkers related to periodontitis (2007)
  • Author(s): Kibayashi M., Tanaka M., Nishida N., Kuboniwa M., Kataoka K., Nagata H., Nakayama K., Morimoto K. Shizukuishi, S
  • Journal Title: J. Periodontol 78(5) Pages: 859-867
  • Peer Reviewed
• [Presentation] NALT CD11b+ Dendritic Cell Migration and Balanced Thl- and Th2-Cytokine Responses Induce Antigen-specific Immunity (2008)
  • Author(s): Sekine S., Fukuyama Y., Kataoka K., Fujihashi K., Kobayashi R., Davidova J., Yamamoto M. , McGhee J. R., Shizukuishi. S., Fujihashi K
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 20081201-03
• [Presentation] Mucosal DCs Activated by Nasal Cholera Toxin Induces Innate IgA CSR of BI B Cells (2008)
  • Author(s): Kataoka K., Fujihashi K., Sekine S., Fukui M., Kawabata S., McGhee J.R., Ito HO., Fujihashi K
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 20081201-03
• [Presentation] Salivary Coltisol and Thl/Th2 Cytokines of Patients with Complaint of Halitosis (2008)
  • Author(s): Fukui M., Baatarjav T., Kataoka K., Hinode D., Ito HO
  • Organizer: 56th JADR Annual Meeting
  • Place of Presentation: Nagoya
  • Year and Date: 20081129-30
• [Presentation] 粘膜アジュバント・コレラ毒素の経鼻投与は唾液腺B1-B細胞のIgAクラススイッチングを誘導する (2008)
  • Author(s): 片岡宏介、関根伸一、福墨、金川裕子、ツエルメグ・バータルジャフ、伊藤博夫
  • Organizer: 第57回口腔衛生学会総会
  • Place of Presentation: 大宮
  • Year and Date: 20081003-05
• [Presentation] 口腔衛生学における基礎研究が社会に寄与できること (2008)
  • Author(s): 片岡宏介
  • Organizer: 第57回口腔衛生学会総会自由集会
  • Place of Presentation: 大宮
  • Year and Date: 20081003-05
• [Presentation] 健常者の唾液中サイトカインの探索的分析 (2008)
  • Author(s): 福差越、片岡宏介、伊藤博夫
  • Organizer: 第57回口腔衛生学会総会
  • Place of Presentation: 大宮
  • Year and Date: 20081003-05
• [Presentation] The Clinical Application of Mucosal Immunology for Preventive Dentistry (2008)
  • Author(s): Kataoka K
  • Organizer: 第56回口腔衛生学会総会自由集会
  • Place of Presentation: 東京
  • Year and Date: 20081003-05
• [Presentation] Nasal Cholera Toxin Up-regulates Mucosal DCs And BJ. B Cell Interactions For The Induction Of Innate IgA CSR (2008)
  • Author(s): Kataoka K., Fujihashi K., Sekine S., Kobayashi R., Gilbert R.L., Ito H., McGhee J.R., Shizukuishi S., Fujihashi K
  • Organizer: The 95^<th> Annual Meeting of The American Association of Immunologists
  • Place of Presentation: San Diego
  • Year and Date: 20080405-07
• [Presentation] Flt3 Ligand Plasmid Enhances Antigen-specific Mucosal and Systemic Immune Responses in Mice Given Nasal Phosphorylcholine (2008)
  • Author(s): Baatarjav T., Kataoka K., Fukui M., Kanagawa H., Ito HO
  • Organizer: The International Symposium on Oral Sciences to Improve the Quality of Life
  • Place of Presentation: Tokushima
  • Year and Date: 2008-09-06
• [Presentation] 金属特異的結合唾液タンパク質の同定 (2008)
  • Author(s): 且岡宏介、関根伸一、東江正裕、田中宗雄、福差誠、便藤博夫、雫石聰
  • Organizer: 第19回口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 徳島
  • Year and Date: 2008-06-22
• [Presentation] 徳島県民の歯科定期健診に対する意識調査 (2008)
  • Author(s): 金川裕子、福差誠、バータルジャフ・ツェルメグ、米津隆仁、横山正秋、安達聡、増田かなめ、片岡宏企、伊藤博夫
  • Organizer: 第回口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 徳島
  • Year and Date: 2008-06-22
• [Presentation] Nasal Flt3 Ligand cDNA Induces Antigen-Specific Immune Responses By Dendritic Cell Recruitment (2008)
  • Author(s): 片岡宏介
  • Organizer: 徳島大学疾患酵素学研究センターセミナー
  • Place of Presentation: 徳島
  • Year and Date: 2008-06-03
• [Presentation] Nasal Cholera Toxin Induces BAFF and APRIL Expressing Dendritic Cells for IgA Class Switching Recombination (2007)
  • Author(s): Kataoka K., Fu jihashi K., Sekine S., Fukuiwa T., Kobayashi R., Tanaka C., McGhee J.R., Shizukuishi S. and Fujihashi K
  • Organizer: 第37回日本免疫学会 総会
  • Place of Presentation: 東京
  • Year and Date: 20071120-22
• [Presentation] nucleaturn Outer Membrane Protein Binds to Salivary Statherin-peptide and Induces Mucosal and Systemic Immune Responses in Mice Given with Cholera Toxin as Mucosal Adjuvant (2007)
  • Author(s): Kataoka K., Nakagaki H., Terao Y., Toe M., Tanaka M., Sekine S., Kawabata S., and Satoshi ShizukuishiFomA, F
  • Organizer: The 93^<rd> Annual Meeting, The American Academy of Periodontology
  • Place of Presentation: Washington DC
  • Year and Date: 20071027-30
• [Presentation] Nasal CT Induces Mucosal DC Activation and BI B Cell IgA CSR For The Enhancement Of Innate S-IgA Antibody Responses (2007)
  • Author(s): Kataoka K., Fujihashi K., Sekine S,, Fukuiwa T., Kobayashi R., Suzuki H., McGhee J. R., Shi zukui shi S. and Fujihashi K.
  • Organizer: 13th International Congress of Mucosal Immunology
  • Place of Presentation: Tokyo
  • Year and Date: 20070709-12
• [Presentation] Nasal Vaccination with New DNA Adjuvants Elicit NALT Dendritic Cell Activation for Prolonged Mucosal Immunity (2007)
  • Author(s): Fukuiwa T., Sekine S., Kobayashi R., Suzuki H., Kataoka K., Boyaka P.N., Kreig A. M., McGhee J.R., Kurono Y. and Fujihashi K
  • Organizer: 13^<th> International Congress of Mucosal Immunology
  • Place of Presentation: Tokyo
  • Year and Date: 20070709-12
• [Presentation] Nasal Immunization with Recombinant Pneumococcal Surface Protein A plus Flt3-Ligand cDNA Provides Protective Immunity against Pneumococcal Pneumonia in Mice (2007)
  • Author(s): Oma K., Oishi K., Kataoka K., Zhao J., Fujihashi K
  • Organizer: 13th International Congress of Mucosal Immunology
  • Place of Presentation: Tokyo
  • Year and Date: 20070709-12
• [Presentation] NALT CD11b+ Dendritic Cell Migration Contributes to Induction of Ag Specific Immunity (2007)
  • Author(s): Sekine S., Kataoka K., Asanuma H., Fukuiwa T., Davydova J., Adachi Y., Kobayashi R., Fujihashi K., Shizukuishi S., Yamamoto M.and Fujihashi K
  • Organizer: The 94^<th> Annual Meeting of The American Association of Immunologists
  • Place of Presentation: Miami
  • Year and Date: 20070518-22
• [Presentation] Contribution of Volatile Organic Compounds to Oral Maldor (2007)
  • Author(s): Nonaka, A., Tanaka M., Noda M., Kataoka K., Kita J. and Shizukuishi S
  • Organizer: The 85th General Session & Exhibition of the International Association for Dental Research
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20070321-24
• [Presentation] 呼気に含まれる揮発性有機化合物のクラスター分析 (2007)
  • Author(s): 野田倫世、田中宗雄、片岡宏介、永田英樹、雫石聰
  • Organizer: 第18回口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-06-17