化学物質が引き起こす野生動物の病態と感受性決定機構の解析

2011 Fiscal Year Annual Research Report

• Project/Area Number: 19671001
• Research Institution: Hokkaido University
• Principal Investigator: 石塚 真由美 北海道大学, 大学院・獣医学研究科, 教授 (50332474)
• Keywords: 環境化学物質 / 野生動物 / アフリカ / シトクロムP450

Research Abstract

環境化学物質は、日常的な曝露レベルであっても、長期曝露によって、ヒトや野生動物に様々な病態を引き起こす。野生動物もヒトと同じく、常に食餌や棲恩環境の汚染を通じて、環境化学物質を生体内に取り込んでいる。そこで、本研究では,「化学物質を起因とする野生動物の病態」について、そめ現状の調査を行うことを目的とした。一方で、残留性汚染物質POPsの分布に国境はなく、その汚染は地球規模で広がっている。特にアフリカでは近年における急激な開発と汚染の進行が懸念されている。そこで、本研究では、野生動物の発癌リスクについて国内における調査はもとより、アフリカについて、初めて汚染物質の実態の把握と、野生動物に対する影響の調査を行うことを目的とした。
本年度は、ザンビア、エジプト、ケニア、ガーナなどより試料を採集し、化学物質汚染に関する分析を進めた。ザンビアにおける鉛やガーナにおけるヒ素など、新たな環境汚染の様式を明らかにすることができた。南アフリカでは、DDTの高濃度汚染地区が明らかとなり、今後重点的な調査が必要であることが分かった。一方、化学物質感受性に関する種差の研究について、哺乳類、鳥類の感受性決定因子のクローニングや、アミノ酸配列によるin silico解析を行った。また、魚類や両生類、鳥類、哺乳類の異物代謝排泄機構に寄与する酵素群やその転写調節因子に関する分子生物学的な分析を行い、新規の代謝酵素を報告したほか、代謝物の種差を同定することができた。

Research Products

• [Journal Article] Molecular cloning and characterization of Th1 and Th2 cytokines of African buffalo (Syncerus caffer) (2012)
  • Author(s): Suzuki S, Konnai S, Okagawa T, Githaka N W, Kariuki E, Gakuya F, Kanduma E, Shirai T, Ikebuchi R, Ikenaka Y, Ishizuka M, Murata S, Ohashi K
  • Journal Title: Int J Immunogenet Volume: 39 Pages: 170-182
  • DOI: 10.1111/j.1744-313X.2011.01062.x
  • Peer Reviewed
• [Journal Article] Sudan III dye strongly induces CYP1A1 mRNA expression in HepG2 cells (2012)
  • Author(s): Ohno, M., Ikenaka, Y., Ishizuka, M.
  • Journal Title: J.Biochem.Mol.Toxicol. Volume: 26 Pages: 16-22
  • DOI: 10.1002/jbt.20408
  • Peer Reviewed
• [Journal Article] Molecular Evidence Predicts Aryl Hydrocarbon Receptor Ligand Insensitivity in the Peregrine Falcon (Falco peregrines) (2012)
  • Author(s): Fujisawa, N., Ikenaka, Y., Kim, E.Y., Lee, J.S., Iwata, H., Ishizuka, M.
  • Journal Title: Eur.J.Wildlife.Res. Volume: 58 Pages: 167-175
  • DOI: 10.1007/s10344-011-0559-1
  • Peer Reviewed
• [Journal Article] All-trans retinoic acid inhibits the recruitment of ARNT to DNA, resulting in the decrease of CYP1A1 mRNA expression in HepG2 cells (2012)
  • Author(s): Ohno, M., Ikenaka, Y., Ishizuka, M.
  • Journal Title: Biochem Biophys Res Commun Volume: 417 Pages: 484-489
  • DOI: 10.1016/j.bbrc.2011.11.146
  • Peer Reviewed
• [Journal Article] Down-Regulation of Hepatic Cytochrome P450 3A in Mice Infected with Babesia microti (2012)
  • Author(s): Shimamoto, Y., Sasaki, M., Ikadai, H., Ishizuka, M., Yokoyama, N., Igarashi, I., Hoshi, F., Kitamura, H.
  • Journal Title: J.Vet.Med.Sci. Volume: 74 Pages: 241-245
  • DOI: 10.1292/jvms.11-0036
  • Peer Reviewed
• [Journal Article] Metal and metalloid contamination in roadside soil and wild rats around a Pb-Zn mine in Kabwe, Zambia (2012)
  • Author(s): Nakayama, S.M.M., Ikenaka, Y., Hamada, K., Muzandu, K., Choongo, K., Teraoka, H., Mizuno, N., Ishizuka, M.
  • Journal Title: Environ.Poll. Volume: 159 Pages: 175-181
  • DOI: 10.1016/j.fct.2011.03.009
  • Peer Reviewed
• [Journal Article] Comparative metabolism of warfarin in rats and chickens (2012)
  • Author(s): Saengtienchai, A., Ikenaka, Y., Watanabe, K., Ishida, T., Ishizuka, M.
  • Journal Title: Poult Sci Volume: 90 Pages: 2775-2781
  • DOI: 10.3382/ps.2010-01257
  • Peer Reviewed
• [Journal Article] Astaxanthin can alter CYP1A-dependent activities via two different mechanisms : Induction of protein expression and inhibition of NADPH P450 reductase dependent electron transfer (2011)
  • Author(s): Ohno, M., Darwish, W.S., Ikenaka, Y., Miki, W., Ishizuka, M
  • Journal Title: Food Chem.Toxicol Volume: 49 Pages: 1285-1291
  • DOI: 10.1016/j.fct.2011.03.009
  • Peer Reviewed
• [Journal Article] Furazolidone induces the activity of microsomal enzymes that metabolize furazolidone in chickens (2011)
  • Author(s): Sasaki, N., Matsumoto, T., Ikenaka, Y., Kazusaka, A., Ishizuka, M., Fujita, S.
  • Journal Title: Pestic.Biochem.Physiol Volume: 100 Pages: 135-139
  • DOI: 10.1016/j.pestbp.2011.02.013
  • Peer Reviewed
• [Journal Article] Phase-II conjugation ability for PAH metabolism in amphibians : Characteristics and inter-species differences (2011)
  • Author(s): Ueda, H., Ikenaka, Y., Nakayama, S.M., Tanaka-Ueno, T., Ishizuka, M.
  • Journal Title: Aquat.Toxicol. Volume: 105 Pages: 337-343
  • DOI: 10.1016/j.aquatox.2011.07.004
  • Peer Reviewed
• [Journal Article] Astaxanthin rich crude extract of Haematococcus pluvialis induces cytochrome P450 1A1 mRNA by activating aryl hydrocarbon receptor in rat hepatoma H4IIE cells (2011)
  • Author(s): Ohno, M., Darwish, W.S., Ikenaka, Y., Miki, W., Fujita, S., Ishizuka, M.
  • Journal Title: Food Chem. Volume: 130 Pages: 356-361
  • Peer Reviewed
• [Journal Article] Uptake of lead, cadmium, and other metals in the liver and kidneys of cattle near a lead-zinc mine in Kabwe, Zambia (2011)
  • Author(s): Yabe, J., Nakayama, S.M., Ikenaka, Y., Muzandu, K., Ishizuka, M., Umemura, T.
  • Journal Title: Environ.Toxicol.Chem. Volume: 30 Pages: 1892-1897
  • DOI: 10.1002/etc.580
  • Peer Reviewed
• [Journal Article] Metal and metalloid levels and bio-accumulation characteristics in soil, sediment, land plants and hippopotami (Hippopotamus amphibius L) from the South Luangwa National Park, Zambia
  • Author(s): Nakayama SM, Ikenaka Y, Muzandu K, Choongo K, M'kandawire E, Yasuda J, Ishizuka M
  • Journal Title: Ecotoxicol.Environ.Safe. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Cytochrome P450 (CYP) in fish
  • Author(s): Uno T, Ishizuka M, Itakura T
  • Journal Title: Environ Toxicol Pharmacol. Volume: (印刷中)
  • DOI: 10.1016/j.etap.2012.02.004
  • Peer Reviewed
• [Presentation] 鳥類における第I相および第II相反応の特徴と種差 (2012)
  • Author(s): 河田みなみ, 池中良徳, 渡邉研右, アクソルン・サエンティエンチャイ, 大野円実, 石塚真由美
  • Organizer: 第153回日本獣医学会
  • Place of Presentation: 大宮ソニックシティー(大宮市)
  • Year and Date: 2012-03-28
• [Presentation] All-trans retinoic acid negatively regulates AHR-mediated CYP1A1 transcription in human hepatoma, HepG2 (2011)
  • Author(s): Marumi Ohno, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 7th Dusseldorf Symposium on Immunotoxicology Biology of the Arylhydrocarbon Receptor-AHR 2011
  • Place of Presentation: The University of Dusseldorf, Dusseldorf, Germany
  • Year and Date: 20110922-20110923
• [Presentation] Species diversity in phase II reaction in polycyclic aromatic hydrocarbons (PAHs) (2011)
  • Author(s): Aksorn Saengtienchai, Yohinori Ikenaka, Michito Shimozuru, Mariko Sajika, Toshio Tsubota, Amnart Poapolathep, Mayumi Ishizuka
  • Organizer: The 14^<th> Seoul National University-Hokkaido University Joint Symposium 21^<st> Century Advances in Veterinary Medical Sciences
  • Place of Presentation: College of Veterinary Medicine, Seoul National University, Seoul, Korea
  • Year and Date: 2011-11-18
• [Presentation] 有害生物の薬剤抵抗性-スーパーラットの汚染環境適応戦略- (2011)
  • Author(s): 石塚真由美
  • Organizer: ペストロジー学会
  • Place of Presentation: 京葉銀行文化プラザ(千葉市)(招待講演)
  • Year and Date: 2011-11-17
• [Presentation] GIS based source estimation of Cu pollution in Lake Itezhi-tezhi and Cu accumulation profile in Oreochromis sp.from the field and laboratory studies (2011)
  • Author(s): Shouta M.M.Nakayama, Yoshinori Ikenaka, Kaampwe Muzandu, Kennedy Choongo, John Yabe, Taro Muroya, Shigeho Ijiri, Takashi Umemura, Maymni Ishizuka
  • Organizer: SETAC North America 32^<nd> Annual Meeting
  • Place of Presentation: Hynes Convention Center (Boston, USA)
  • Year and Date: 2011-11-15
• [Presentation] 汚染環境下の野生動物の適応と毒性 (2011)
  • Author(s): 石塚真由美、池中良徳
  • Organizer: フォーラム2011・衛生薬学環境トキシコロジーシンポジウム
  • Place of Presentation: 金沢エクセルホテル東急(金沢)(招待講演)
  • Year and Date: 2011-10-28
• [Presentation] アカハライモリとアフリカツメガエルにおける異物代謝酵素の比較 (2011)
  • Author(s): 上田春樹、中山翔太、川合佑典、田中-上野寛子、池中良徳・石塚真由美
  • Organizer: 日本爬虫両棲類学会第50回大会
  • Place of Presentation: 京都大学北部構内 理学部(京都)
  • Year and Date: 2011-10-08
• [Presentation] ラットの消化管における異物代謝酵素の比較 (2011)
  • Author(s): 糸谷悠美、池中良徳、大野円実、中山翔太、ワギソベイ、石塚真由美
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学(堺市)
  • Year and Date: 2011-09-21
• [Presentation] ラットにおける薬物代謝酵素シトクロムP450の系統差について (2011)
  • Author(s): 西山義広、池中良徳、大野円実、渡邉研右、川合佑典、中山翔太、田中和之、石塚真由美
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学(堺市)
  • Year and Date: 2011-09-21
• [Presentation] レチノイン酸はAH受容体介在の遺伝子転写調節機構を阻害する (2011)
  • Author(s): 大野円実、池中良徳、石塚真由美
  • Organizer: 第25回カロテノイド研究談話会
  • Place of Presentation: 茨城県つくば市つくば国際会議場
  • Year and Date: 2011-09-13
• [Presentation] Effects of current malaria control in lake environment ; The levels of Organochlorine pesticide and toxic metals in fish species from Lake Awassa, Ethiopia (2011)
  • Author(s): Yoshinori IKENAKA, Yared Beyene, Kensuke WATANABE, Aksorn Saengtienchai, Shouta NAKAYAMA, Mayumi ISHIZUKA
  • Organizer: 3rd International Toxicology Symposium in Africa
  • Place of Presentation: University of Zambia (Lusaka, Zambia)
  • Year and Date: 2011-09-08
• [Presentation] GIS-based source estimation of Cu pollution in Lake Itezhi-tezhi and metal accumulation profiles in Oreochromis sp.and other fish species from both and laboratory studies (2011)
  • Author(s): Shouta M.M.Nakayama, Yoshinori Ikenaka, Kaampwe Muzandu, Kennedy Choongo, John Yabe, Taro Muroya, Shigeho Ijiri, Takashi Umemura, Mayumi Ishizuka
  • Organizer: 3rd International Toxicology Symposium in Africa
  • Place of Presentation: University of Zambia (Lusaka, Zambia)
  • Year and Date: 2011-09-08
• [Presentation] Metal concentration and stable isotope ratio analysis (d13C and d15N) in water, sediment and fish from Lake Itezhi-tezhi, Zambia (2011)
  • Author(s): Shouta M.M.Nakayama, Yoshinori Ikenaka, Kaampwe Muzandu, Kennedy Choongo, John Yabe, Taro Muroya, Takashi Umemura, Mayumi Ishizuka
  • Organizer: 第20回環境化学討論会
  • Place of Presentation: 熊本県立大学(熊本)
  • Year and Date: 2011-07-17
• [Presentation] Metal and metalloid levels and bio-accumulation characteristics in soil, sediment, land plants and hippopotami (Hippopotamus amphibius L) from the South Luangwa National Park, Zambia (2011)
  • Author(s): Shouta M.M.Nakayama, Yoshinori Ikenaka, Kaampwe Muzandu, Kennedy Choongo, Ethel M'kandawire, Jun Yasuda, Mayumi Ishizuka
  • Organizer: 第20回環境化学討論会
  • Place of Presentation: 熊本県立大学(熊本)
  • Year and Date: 2011-07-17
• [Presentation] Effects of current malaria control in lake environment ; The levels of Organochlorine pesticide and toxic metals in fish species from Lake Awassa, ETHIOPIA (2011)
  • Author(s): YARED Beyene, Yoshinori IKENAKA, Kensuke WATANABE, Aksorn SAENGT IENCHAI, Shouta NAKAYAMA, Mayumi ISHIZUKA
  • Organizer: 第20回環境化学討論会
  • Place of Presentation: 熊本県立大学(熊本)
  • Year and Date: 2011-07-17
• [Presentation] ガーナの金鉱山周辺(オブアシ自治区)における金属汚染と野生ラットにおける毒性影響評価 (2011)
  • Author(s): 大谷豪, 池中良徳, 中山翔太, Osei Akoto, Joseph APAU, Elvis BAIDOO, 石塚真由美
  • Organizer: 第20回環境化学討論会
  • Place of Presentation: 熊本県立大学(熊本)
  • Year and Date: 2011-07-17
• [Presentation] Species diversity in phase II reaction of pyrene metabolism (2011)
  • Author(s): SAENGTIENCHAI, Yoshinori IKENAKA, Mayumi ISHIZUKA
  • Organizer: 第38回日本トキシコロジー学会
  • Place of Presentation: Attractive Convention Complex, Pacifico Yokohama (Japan)
  • Year and Date: 2011-07-12
• [Presentation] 多環芳香族炭化水素類(PAHs)に対する魚類の異物代謝機構の解明:魚類で観察された第II相抱合反応の特徴と種差 (2011)
  • Author(s): 小栗真美, 池中良徳, Aksorn Saengtienchai, 上田春樹, 石塚真由美
  • Organizer: 第38回日本トキシコロジー学会
  • Place of Presentation: Attractive Convention Complex, Pacifico Yokohama (Japan)
  • Year and Date: 2011-07-12
• [Presentation] All-trans retinoic acid negatively regulates AHR-mediated CYP1A1 transcription in human hepatoma, HepG2 (2011)
  • Author(s): Marumi Ohno, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 17th International Symposium on Cytochrome P450
  • Place of Presentation: The University of Manchester, Manchester (UK)
  • Year and Date: 2011-06-27
• [Presentation] Metal concentration and stable isotope ratio analysis (d13C and d15N) in water, sediment and fish from Lake Itezhi-tezhi, Zambia (2011)
  • Author(s): Shouta M.M.Nakayama, Yoshinori Ikenaka, Kaampwe Muzandu, Kennedy Choongo, Jun Yasuda, Mayumi Ishizuka
  • Organizer: 3rd CSTS and 5th SETAC-Africa Joint Toxicology Conference
  • Place of Presentation: University of Buea (Buea, Cameroon)
  • Year and Date: 2011-06-03
• [Presentation] Concentration and bioaccumulation of 10 metals in soil, river sediment, plant and hippopotamus (Hippopotamus amphibius L) in the South Luangwa National Park, Zambia (2011)
  • Author(s): Shouta M.M.Nakayama, Yoshinori Ikenaka, Kaampwe Muzandu, Kennedy Choongo, Jun Yasuda, Mayumi Ishizuka
  • Organizer: 3rd CSTS and 5th SETAC-Africa Joint Toxicology Conference
  • Place of Presentation: University of Buea (Buea, Cameroon)
  • Year and Date: 2011-06-02