Study of the function and structure of single inhibitory synapses revealed by two-photon uncaging of GABA

2009 Fiscal Year Self-evaluation Report

• Project/Area Number: 19680020
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology and muscle physiology
• Research Institution: The University of Tokyo
• Principal Investigator: MATSUZAKI Masanori The University of Tokyo, 大学院・医学系研究科, 准教授 (50353438)
• Project Period (FY): 2007 – 2010
• Keywords: ニューロン / シナプス機能

Research Abstract

本研究では、2光子励起ケイジドGABA活性化法を新たに開発し、これをラット大脳海馬及び新皮質の興奮性細胞に適用し、単一シナプスレベルでの機能的GABA受容体の細胞全体にわたるマッピングを行い、単一抑制性シナプス後部の機能・形態を明らかにすることを目的としている。

Research Products

• [Journal Article] Two-photon uncaging of γ-aminobutyric acid in intact brain tissue. (2010)
  • Author(s): Matsuzaki M., Hayama T., Kasai H., #Ellis-Davies G.C.R.
  • Journal Title: Nature Chemical Biology 6 Pages: 255-257
  • Peer Reviewed
• [Journal Article] Two-color, two-photon uncaging of glutamate and GABA. (2010)
  • Author(s): Kantevari S., *Matsuzaki M., Kanemoto Y., Kasai H., Ellis-Davies G.C.R.
  • Journal Title: Nature Methods 7 Pages: 123-125
  • Peer Reviewed
• [Journal Article] Protein-synthesis and neurotrophin dependent structural plasticity of single dendritic spines. (2008)
  • Author(s): Tanaka J., Horiike Y., Matsuzaki M., Miyazaki T., Ellis-Davies G.C.R., Kasai H.
  • Journal Title: Science 319 Pages: 1683-1687
  • Peer Reviewed
• [Journal Article] Ellis-Davies G.C.R. and Kasai H. The subspine organization of actin fibers regulates the structure and plasticity of dendritic spines. (2008)
  • Author(s): Honkura N., Matsuzaki M., Noguchi J.
  • Journal Title: Neuron 57 Pages: 719-729
  • Peer Reviewed
• [Journal Article] 4-carboxymethoxy-5, 7-dinitroindolinyl-glu: an improved caged glutamate for expeditious ultraviolet and 2-photon photolysis in brain slices. (2007)
  • Author(s): Ellis-Davies G.. C.R., Matsuzaki M., Paukert M., Kasai H., Bergles D.E.
  • Journal Title: Journal of Neuroscience 27 Pages: 6601-6604
  • Peer Reviewed
• [Presentation] Optical Stimulation of synapses and neurons. (2009)
  • Author(s): Matsuzaki M.
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: Tokushima
  • Year and Date: 2009-11-01
• [Remarks]
  • URL: http://www.bm2.m.u-tokyo.ac.jp/