2010 Fiscal Year Final Research Report
Vibrational spectroscopy of single proteins
| Project/Area Number |
19685001
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
FUJIYOSHI Satoru Tokyo Institute of Technology, 大学院・理工学研究科, 助教 (70371705)
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| Project Period (FY) |
2007 – 2011
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| Keywords | 分光 / 低温 / タンパク質 / 単一分子分光 / 赤外 / 中赤外 / アミドI |
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| Research Abstract |
Vibrational infrared absorption of a single protein molecule was detected at a few kelvins as infrared-induced recovery of visible fluorescence of a dye with which the protein was labeled. This sensitive method of detecting infrared absorption was demonstrated for a single bovine serum albumin (BSA) molecule labeled with Alexa Fluor 660 by determining the vibrational infrared absorption spectrum of the backbone vibrations of the R-helical structure in the wavelength region around 6μm (1650cm-1). In addition to measuring the vibrational infrared absorption spectrum, the visible fluorescence can be simultaneously used for imaging of the same dye-labeled single protein molecules.
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