The molecular mechanisms of heterochromatin establishment and maintenance

2010 Fiscal Year Final Research Report

• Project/Area Number: 19687001
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NAKAYAMA Jun-ichi The Institute of Physical and Chemical Research, クロマチン動態研究チーム, チームリーダー (60373338)
• Project Period (FY): 2007 – 2010
• Keywords: 遺伝子 / 発現制御 / ヘテロクロマチン / RNA干渉 / 分裂酵母

Research Abstract

In this research program, we tried to gain a better understanding of the molecular mechanisms underlying the establishment and maintenance of higher-order chromatin structure, heterochromatin. Using fission yeast as an model organism, we employed an genetic screening and successfully identified novel RNAi-related factors involved in heterochromatin assembly. Further genetic analyses of mutant cells uncovered a novel insight into the molecular mechanisms of how RNAi effector complexes are recruited to the heterochromatin.

Research Products

• [Journal Article] Roles of fission yeast Grc3 protein in ribosomal RNA processing and heterochromatic gene silencing. (2011)
  • Author(s): Kitano, E., Hayashi, A., Kanai, D., Shinmyozu, K., Nakayama, J.
  • Journal Title: J.Biol.Chem. 286 Pages: 15391-15402
  • Peer Reviewed
• [Journal Article] N-terminal phosphorylation of HP1α promotes its chromatin binding. (2011)
  • Author(s): Hiragami-Hamada, K., Shinmyozu, K., Hamada, D., Tatsu, Y., Uegaki, S., Fujiwara, S., Nakayama, J.
  • Journal Title: Mol.Cell.Biol. 31 Pages: 1186-1200
  • Peer Reviewed
• [Journal Article] Physiological roles of Class I HDAC complex and Histone demethylase. (2011)
  • Author(s): Hayakawa, T., Nakayama, J.
  • Journal Title: J.Biomed.Biotech. 2011 Pages: 129383
  • Peer Reviewed
• [Journal Article] Methylation of ribosomal protein L42 regulates ribosomal function and stress-adapted cell growth. (2010)
  • Author(s): Shirai, A., Sadaie, M., Shinmyozu, K., Nakayama, J.
  • Journal Title: J.Biol.Chem. 285 Pages: 22448-22460
  • Peer Reviewed
• [Journal Article] R.MRG15 directly binds to PALB2 and stimulates homology-directed repair of chromosomal breaks. (2010)
  • Author(s): Hayakawa, T., Zhang, F., Hayakawa, N., Ohtani, Y., Shinmyozu, K., Nakayama, J., Andressen, P.
  • Journal Title: J.Cell Sci. 123 Pages: 1124-1130
  • Peer Reviewed
• [Journal Article] Reconstitution of Arabidopsis thaliana SUMO pathways in E.coli : functional evaluation of SUMO machinery proteins and mapping of SUMOylation sites by mass spectrometry. (2009)
  • Author(s): Okada, S., Nagabuchi, M., Takamura, Y., Nakagawa, T., Shinmyozu, K., Nakayama, J., Tanaka, K.
  • Journal Title: Plant Cell Physiol. 50 Pages: 1049-1061
  • Peer Reviewed
• [Journal Article] Phosphorylation of Swi6/HP1 regulates transcriptional gene silencing at heterochromatin. (2009)
  • Author(s): Shimada, A., Dohke, K., Sadaie, M., Shinmyozu, K., Nakayama, J., Urano, T., Murakami, Y.
  • Journal Title: Genes Dev. 23 Pages: 18-23
  • Peer Reviewed
• [Journal Article] The heterochromatin protein Swi6/HP1 activates replication origins at pericentromeres and silent mating-type locus. (2009)
  • Author(s): Hayashi, M.T., Takahashi, T.S., Nakagawa, T., Nakayama, J., Masukata, H.
  • Journal Title: Nat.Cell Biol. 11 Pages: 357-362
  • Peer Reviewed
• [Journal Article] Balance between distinct HP1 family proteins controls heterochromatin assembly in fission yeast. (2008)
  • Author(s): Sadaie, M., Kawaguchi, R., Ohtani, Y., Arisaka, F., Tanaka, K., Shirahige, K., Nakayama, J.
  • Journal Title: Mol.Cell.Biol. 28 Pages: 6973-6988
  • Peer Reviewed
• [Journal Article] siRNA-mediated heterochromatin establishment requires HP1 and is associated with antisense transcription. (2008)
  • Author(s): Iida, T., Nakayama, J., Moazed, D.
  • Journal Title: Mol.Cell 31 Pages: 178-189
  • Peer Reviewed
• [Journal Article] A conserved SET-domain methyl transferase, Set11, modifies ribosomal protein Rpl12 in fission yeast. (2008)
  • Author(s): Sadaie, M., Shinmyozu, K., Nakayama, J.
  • Journal Title: J.Biol.Chem. 283 Pages: 7185-7195
  • Peer Reviewed
• [Journal Article] Gene expression and distribution of Swi6 in partial aneuploids of the fission yeast Schizosaccharomyces pombe. (2008)
  • Author(s): Chikashige, Y., Nakayama, J., Hiraoka, Y.
  • Journal Title: Cell Struct.Funct. 32 Pages: 149-161
  • Peer Reviewed
• [Presentation] 高次クロマチン構造形成におけるクロモドメインタンパク質の役割 (2010)
  • Author(s): 中山潤一
  • Organizer: 第33回日本分子生物学会,第83回日本生化学会合同年会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-09
• [Presentation] Chromodomain proteins and RNAi-mediated heterochromatin assembly (2009)
  • Author(s): 中山潤一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-09
• [Presentation] Distinct roles of chromodomain proteins in the formation of higher-order chromatin structure (2009)
  • Author(s): 中山潤一
  • Organizer: The 5th International Fission Yeast Meeting
  • Place of Presentation: 東京都
  • Year and Date: 2009-10-29
• [Presentation] Distinct roles of chromodomain proteins in the formation of higher-order chromatin structure (2008)
  • Author(s): 中山潤一
  • Organizer: 第21回内藤カンファレンス
  • Place of Presentation: 北杜
  • Year and Date: 2008-06-26
• [Presentation] 高次クロマチン構造形成とRNA代謝ネットワーク (2008)
  • Author(s): 中山潤一
  • Organizer: 第2回日本エピジェネティクス研究会年会
  • Place of Presentation: 三島市
  • Year and Date: 2008-05-10
• [Presentation] 分裂酵母ヘテロクロマチン構造形成には2つのHP1タンパク質の別個の機能が必要である (2007)
  • Author(s): 中山潤一
  • Organizer: 第30回日本分子生物学会年会,第80回日本生化学会合同年会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-12-12
• [Book] 細胞工学2011年7月号(「高次クロマチン構造とノンコーディングRNA」) (2011)
  • Author(s): 中山潤一
  • Publisher: 学研メディカル秀潤社(印刷中)
• [Book] エピジェネティクス(堀越正美 監訳)(「3章 概観と概念(Overview and Concept)」) (2010)
  • Author(s): 中山潤一
  • Total Pages: 27-73
  • Publisher: 培風館
• [Book] 蛋白質核酸酵素増刊「染色体サイクル」(正井久雄、升方久夫、釣本敏樹、仁木宏典、篠原彰編),54巻(染色体サイクルのクロマチン制御:概論) (2009)
  • Author(s): 中山潤一
  • Total Pages: 485-487
  • Publisher: 共立出版
• [Book] 蛋白質核酸酵素2008年6月号,53巻(「高次クロマチンの形成機構とエピジェネティック制御」) (2008)
  • Author(s): 中山潤一
  • Total Pages: 801-808
  • Publisher: 共立出版
• [Book] RNA実験ノート(稲田利文・塩見春彦編)(「小分子RNAとクロマチン動態」無敵のバイオテクニカルシリーズ) (2008)
  • Author(s): 石田真由美・中山潤一
  • Total Pages: 16-17
  • Publisher: 羊土社
• [Book] 蛋白質核酸酵素2007年6月号,52巻(「ヒストンのメチル化修飾と高次クロマチン構造の形成機構」) (2007)
  • Author(s): 定家真人・中山潤一
  • Total Pages: 739-746
  • Publisher: 共立出版
• [Book] 実験医学増刊「染色体サイクル」(正井久雄・渡辺良典編),25巻(RNAiによるヘテロクロマチン制御機構) (2007)
  • Author(s): 飯田哲史・中山潤一
  • Total Pages: 186-190
  • Publisher: 羊土社