2010 Fiscal Year Final Research Report
Genetic analysis of hybrid breakdown in rice
| Project/Area Number |
19688002
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | Nagoya University |
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Principal Investigator |
ASHIKARI Motoyuki Nagoya University, 生物機能開発利用研究センター, 教授 (80324383)
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| Project Period (FY) |
2007 – 2010
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| Keywords | イネ / 生殖的隔離 |
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| Research Abstract |
Reproductive barriers are important for the maintenance of species identity. We discovered a reproductive barrier via hybrid breakdown among the progeny of a cross between the japonica rice cultivar Koshihikari and the indica rice cultivar Habataki. Genetic analysis indicated that the hybrid breakdown is regulated by the interaction of two recessive genes: hbd2 in Habataki and hbd3 in Koshihikari. Linkage mapping showed that hbd2 is located near the 100 cM region of chromosome 2 in Habataki, whereas hbd3 is located near the 60 cM region of chromosome 11 in Koshihikari. Fine mapping of hbd2 resulted in the identification of the causal gene as casein kinase I (CKI1). Further analysis revealed that hbd2-CKI1 allele gains its deleterious function that causes the weakness phenotype by a change of one amino acid. As for the other gene, hbd3 was mapped to the NBS-LRR gene cluster region. It is the most common class of R-gene that triggers the immune signal in response to pathogen attack. Expression analysis of pathogen response marker genes suggested that weakness phenotype in this hybrid breakdown can be attributed to an autoimmune response. This finding provides a new insight in understanding the molecular and evolutionary mechanisms establishing reproductive barriers in plants.
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Research Products
(5 results)
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[Journal Article] Interaction of two recessive genes, hbd2 and hbd3, induces hybrid breakdown in rice.2007
Author(s)
Yamamoto, E., Takashi, T., Morinaka, Y., Lin, S., Kitano, H., Matsuoka, M., Ashikari, M.
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Journal Title
Theoretical and applied genetics. 115
Pages: 187-194
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[Presentation] イネにおける自己免疫応答と生殖的隔離2010
Author(s)
山本英司, 高師知紀, 森中洋一, 林少揚, 呉健忠, 松本隆, 北野英己, 松岡信, 芦苅基行
Organizer
イネ遺伝学・分子生物学ワークショップ2010
Place of Presentation
名古屋大学野依記念学術交流館
Year and Date
2010-07-12
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