2010 Fiscal Year Final Research Report
Developing phytoplankton detection and monitoring methods in lakes
| Project/Area Number |
19688011
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Lake Biwa Environmental Research Institute |
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Principal Investigator |
ISHIKAWA Kanako Lake Biwa Environmental Research Institute, 総合解析部門, 主任研究員 (80393180)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 環境微生物 / 分子識別法 / プランクトン / 環境監視 / 琵琶湖 |
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| Research Abstract |
A plankton DNA database and monitoring method were developed in this project. Plankton cultures were isolated from Lake Biwa, and 18S or 16S rRNA genes were amplified with each universal primer set. The precise sequence of nucleotides in a sample of SSU-rRNA gene was analyzed after TA cloning treatment. Primers for real-time PCR to detect 18 target species from Lake Biwa were designed by Primer 3 software based on the alignment of the Lake Biwa Plankton DNA Database using DNASIS Pro V2.10. These primer sets were tested using a real time PCR system, and the results of 15 species' detection were synchronized using a microscope. PCR-DGGE (Denaturing Gradient Gel Electrophoresis) analysis successfully showed pico-plankton species that have not been previously detected in Lake Biwa. We believe that these molecular methods will be useful for future diversity studies as well as for long-term plankton monitoring.
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