Quantitative proteomics of membrane transporters using application of a simultaneous LC/MS/MS method

2009 Fiscal Year Self-evaluation Report

• Project/Area Number: 19689028
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Tohoku University
• Principal Investigator: ONOGAWA Tohru Tohoku University, 病院, 助教 (50431557)
• Project Period (FY): 2007 – 2010
• Keywords: 薬物輸送体 / 抗癌剤感受性 / プロテオミクス / バイオマーカー

Research Abstract

本研究の目的は、当科手術検体を用いて、それぞれの癌細胞における機能膜蛋白質である複数の薬物輸送体及び代謝酵素蛋白群の発現をプロテオミクスにて網羅的同時絶対定量し、その特異的蛋白発現プロファイルと臨床における抗癌剤治療成績との相関を検討することにより、新規抗癌剤感受性予測システムを構築し、その臨床応用を目指すことにある。

Research Products

• [Journal Article] Up-regulated p27Kip1 reduces matrix metalloproteinase-9 and inhibits invasion of human breast cancer cells. (2008)
  • Author(s): Mizuma M, Katayose Y, Yamamoto K, Shiraso S, Sasaki T, Yabuuchi S, Oda A, Masuda K, Rikiyama T, Onogawa T, Ohtsuka H, Motoi F, Egawa S, Unno M.
  • Journal Title: Anticancer Res. 28(5A) Pages: 2669-2677
  • Peer Reviewed
• [Journal Article] Bile acids repress E-cadherin through the induction of Snail and increase cancer invasiveness in human hepatobiliary carcinoma. (2008)
  • Author(s): Fukase K, Ohtsuka H, Onogawa T, Oshio H, Ii T, Mutoh M, Katayose Y, Rikiyama T, Oikawa M, Motoi F, Egawa S, Abe T, Unno M.
  • Journal Title: Cancer Sci. 99(9) Pages: 1785-1792
  • Peer Reviewed
• [Journal Article] Peroxisome proliferator-activated receptor alpha activates cyclooxygenase-2 gene transcription through bile acid transport in human colorectal cancer cell lines. (2008)
  • Author(s): Oshio H, Abe T, Onogawa T, Ohtsuka H, Sato T, Ii T, Fukase K, Muto M, Katayose Y, Oikawa M, Rikiyama T, Egawa S, Unno M.
  • Journal Title: J Gastroenterol. 43(7) Pages: 538-549
  • Peer Reviewed
• [Journal Article] Human liver-specific organic anion transporter-2 is a potent prognostic factor for human breast carcinoma. (2007)
  • Author(s): Onogawa T, Muto M, Suzuki T, Ishida T, Rikiyama T, Katayose Y, Ohuchi N, Sasano H, Abe T, Unno M.
  • Journal Title: Cancer Sci. 98(10) Pages: 1570-1576
  • Peer Reviewed
• [Journal Article] Expression of organic cation transporter SLC22A16 in human epithelial ovarian cancer: a possible role of the adriamycin importer. (2007)
  • Author(s): Ota K, Ito K, Akahira J, Sato N, Onogawa T, Moriya T, Unno M, Abe T, Niikura H, Takano T, Yaegashi N.
  • Journal Title: Int J Gynecol Pathol. 26(3) Pages: 334-340
  • Peer Reviewed
• [Presentation] 胆道癌ホルマリン固定パラフィン包埋切片からのディスカバリープロテオミクス (2009)
  • Author(s): 小野川徹, ら(21名中1番目)
  • Organizer: 日本ヒトプロテオーム機構第7回大会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-27
• [Presentation] 胆道癌ホルマリン固定パラフィン包埋組織からのディスカバリープロテオミクス (2009)
  • Author(s): 小野川徹, ら(21名中1番目)
  • Organizer: 第5回日本臨床プロテオーム研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-09
• [Presentation] 新規乳癌独立予後規定因子LST-2/SLCO1B3の癌細胞特異的発現とその腫瘍生物学的意義 (2008)
  • Author(s): 小野川徹, ら(8名中1番目)
  • Organizer: 第3回トランスポーター研究会年会
  • Place of Presentation: 京都
  • Year and Date: 2008-06-07
• [Presentation] 検体微量ペプチド試料からの薬物輸送体及び代謝酵素蛋白群の網羅的同時絶対定量解析と抗癌剤感受性予測 (2008)
  • Author(s): 小野川徹ら(18名中1番目)
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2008-05-15
• [Book] Diseases of the Pancreas -Current Surgical Therapy (2008)
  • Author(s): Beger HG, Matsuno S, Cameron JL Eds.
  • Total Pages: 895-904
  • Publisher: Springer-Verlag