Analysis of Molecular Mechanism of Female Reproductive Organ Tissue Stem Cell Systems for clinical application

2009 Fiscal Year Final Research Report

• Project/Area Number: 19689034
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: KAJITANI Takashi Keio University, 医学部, 助教 (60407111)
• Project Period (FY): 2007 – 2009
• Keywords: 再生医学 / 幹細胞学 / 子宮内膜 / 子宮筋

Research Abstract

In this project, we have investigated the molecular mechanisms of the physiological function of the endometrial- and myometrial tissue stem cells, by profiling the specific gene expression pattern of the putative stem cells, and by analyzing each gene's function. The results of this project may solve the some problems for the clinical usage of the stem cells.

Research Products

• [Journal Article] Human uterine stem/progenitor cells: their possible role in uterine physiology. (2010)
  • Author(s): Maruyama T, Masuda H, Ono M, Kajitani T, Yoshimura Y
  • Journal Title: Reproduction (in press.)
  • Peer Reviewed
• [Journal Article] Stem cell-like properties of the endometrial side population: implication in endometrial regeneration. (2010)
  • Author(s): Masuda H, Matsuzaki Y, Hiratsu E, Ono M, Nagashima T, Kajitani T, Arase T, Oda H, Uchida H, Asada H, Ito M, Yoshimura Y, Maruyama T, Okano H
  • Journal Title: PLoS One 5(4) Pages: e10387
  • Peer Reviewed
• [Journal Article] The UDP-glucose receptor P2RY14 triggers innate mucosal immunity in the female reproductive tract by inducing IL-8. (2009)
  • Author(s): Arase T, Uchida H, Kajitani T, Ono M, Tamaki K, Oda H, Nishikawa S, Kagami M, Nagashima T, Masuda H, Asada H, Yoshimura Y, Maruyama T
  • Journal Title: J Immunol. 182(11) Pages: 7074-7084
  • Peer Reviewed
• [Journal Article] Glycodelin blocks progression to S phase and inhibits cell growth: a possible progesterone-induced regulator for endometrial epithelial cell growth. (2008)
  • Author(s): Ohta K, Maruyama T, Uchida H, Ono M, Nagashima T, Arase T, Kajitani T, Oda H, Morita M, Yoshimura Y
  • Journal Title: Mol Hum Reprod. 14(1) Pages: 17-22
  • Peer Reviewed
• [Journal Article] Cyp11b1 is induced in the murine gonad by luteinizing hormone/ human chorionic gonadotropin and involved in the production of 11-ketotestosterone, a major fish androgen; conservation and evolution of androgen metabolic pathway. (2008)
  • Author(s): Yazawa T, Uesaka M, Inaoka Y, Mizutani T, Sekiguchi T, Kajitani T, Kitano T, Umezawa A, Miyamoto K
  • Journal Title: Endocrinology. 149(4) Pages: 1786-1792
  • Peer Reviewed
• [Journal Article] Activation of SRC kinase and phosphorylation of STAT5 are required for decidual transformation of human endometrial stromal cells. (2008)
  • Author(s): Nagashima T, Maruyama T, Uchida H, Kajitani T, Arase T, Ono M, Oda H, Kagami M, Masuda H, Nishikawa S, Asada H, Yoshimura Y
  • Journal Title: Endocrinology. 149(3) Pages: 1227-1234
  • Peer Reviewed
• [Journal Article] Analysis of serum FSH bioactivity in a patient with an FSH-secreting pituitary microadenoma and multicystic ovaries: A Case Report. (2008)
  • Author(s): Kajitani T, Liu S, Maruyama T, Uchida H, Sakurai R, Masuda H, Nagashima T, Ono M, Arase T, Yoshimura Y
  • Journal Title: Hum Reprod. 23(2) Pages: 435-439
  • Peer Reviewed
• [Journal Article] 子宮内膜症・腺筋症由来疼痛に関連する遺伝子の発現解析. (2008)
  • Author(s): 梶谷宇, 丸山哲夫, 浅田弘法, 小野政徳, 荒瀬透, 小田英之, 西川明花, 内田浩, 吉村泰典
  • Journal Title: エンドメトリオーシス研会誌 29 Pages: 91-93
  • Peer Reviewed
• [Journal Article] 宮内膜症の病因に関する基礎研究子宮内膜症関連遺伝子同定のための新しいアプローチ. (2008)
  • Author(s): 丸山哲夫, 梶谷宇, 小田英之, 西川明花, 荒瀬透, 内田浩, 浅田弘法, 吉村泰典
  • Journal Title: エンドメトリオーシス研会誌 29 Pages: 42
  • Peer Reviewed
• [Journal Article] ヒト子宮内膜様幹細胞の同定・分離とその幹細胞特性の機能解析. (2008)
  • Author(s): 升田博隆, 丸山哲夫, 小野政徳, 長島隆, 梶谷宇, 内田浩, 浅田弘法, 岡野栄之, 松崎有未, 吉村泰典
  • Journal Title: 日本内分泌学会雑誌 84(2) Pages: 626-627
  • Peer Reviewed
• [Journal Article] 妊娠子宮における子宮筋Side Population細胞の役割. (2008)
  • Author(s): 小野政徳, 丸山哲夫, 梶谷宇, 内田浩, 荒瀬透, 各務真紀, 小田英之, 西川明花, 升田博隆, 長島隆, 太田邦明, 伊藤守, 浅田弘法, 岡野栄之, 松崎有未, 吉村泰典
  • Journal Title: 日本生殖内分泌学会雑誌 13 Pages: 25-29
  • Peer Reviewed
• [Journal Article] 異所性子宮内膜症. (2008)
  • Author(s): 浅田弘法, 古谷正敬, 西尾浩, 升田博隆, 内田浩, 丸山哲夫, 梶谷宇, 木挽貢慈, 吉村泰典
  • Journal Title: 産婦人科治療 96(3) Pages: 241-247
  • Peer Reviewed
• [Journal Article] 子宮内膜脱落膜化の機序の解明-チロシンキナーゼSRCの役割と意義- (2008)
  • Author(s): 丸山哲夫, 長島隆, 梶谷宇, 内田浩, 吉村泰典
  • Journal Title: 産婦人科の実際 57(2) Pages: 193-198
  • Peer Reviewed
• [Journal Article] Side population in human uterine myometrium displays phenotypic and functional characteristics of myometrial stem cells. (2007)
  • Author(s): Ono M, Maruyama T, Masuda H, Kajitani T, Nagashima T, Arase T, Ito M, Ohta K, Uchida H, Asada H, Yoshimura Y, Okano H, Matsuzaki Y
  • Journal Title: Proc Natl Acad Sci USA. 104(47) Pages: 18700-18705
  • Peer Reviewed
• [Journal Article] Histone deacetylase inhibitor-induced glycodelin enhances the initial step of implantation. (2007)
  • Author(s): Uchida H, Maruyama T, Ohta K, Ono M, Arase T, Kagami M, Oda H, Kajitani T, Asada H, Yoshimura Y
  • Journal Title: Hum Reprod. 22(10) Pages: 2615-2622
  • Peer Reviewed
• [Journal Article] Histone acetylation and subcellular localization of chromosomal protein BRD4 during mouse oocyte meiosis and mitosis. (2007)
  • Author(s): Nagashima T, Maruyama T, Furuya M, Kajitani T, Uchida H, Masuda H, Ono M, Arase T, Ozato K, Yoshimura Y
  • Journal Title: Mol Hum Reprod. 13(3) Pages: 1-8
  • Peer Reviewed
• [Journal Article] Histone deacetylase inhibitors stimulate cell migration in human endometrial adenocarcinoma cells through up-regulation of glycodelin. (2007)
  • Author(s): Uchida H, Maruyama T, Ono M, Ohta K, Kajitani T, Masuda H, Nagashima T, Arase T, Asada H, Yoshimura Y
  • Journal Title: Endocrinology. 148(2) Pages: 896-902
  • Peer Reviewed
• [Journal Article] 子宮内膜と幹細胞. (2007)
  • Author(s): 丸山哲夫, 升田博隆, 梶谷宇, 小野政徳, 吉村泰典
  • Journal Title: 産科と婦人科 74 Pages: 1635-1643
  • Peer Reviewed
• [Presentation] 腹水と血清中における代謝物質の網羅的分析による子宮内膜症特異的な代謝物質の探索. (2010)
  • Author(s): 川田陽子、島田智恵、浅田弘法、杉本昌弘、平山明由、阿部しのぶ、古谷正敬、内田浩、浜谷敏生、丸山哲夫、梶谷宇、久慈直昭、吉村泰典、曽我朋義、富田勝
  • Organizer: 第31回日本エンドメトリオーシス学会
  • Place of Presentation: 京都
  • Year and Date: 20100116-20100117
• [Presentation] FSH産生下垂体腫瘍症例における卵巣過剰刺激の発症メカニズムの解析. (2009)
  • Author(s): 西川明花、丸山哲夫、梶谷宇、内田浩、各務真紀、小田英之、宮崎薫、吉村泰典
  • Organizer: 第55回日本生殖医学会学術講演会
  • Place of Presentation: 金沢
  • Year and Date: 20091121-20091123
• [Presentation] Up-regulation of 5- and 15-lipoxygenases in endometriosis (2009)
  • Author(s): Kajitani T, Maruyama T, Asada H, Yamasaki A, Oda H, Nishikawa S, Uchida H, Yoshimura Y
  • Organizer: he 25th Annual Meeting of the European Society of Human Reproduction and Embryology
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 20090628-20090701
• [Presentation] 新しい方法で単離された子宮平滑筋幹細胞の妊娠子宮リモデリングにおける役割. (2009)
  • Author(s): 小野政徳、丸山哲夫、梶谷宇、内田浩、荒瀬透、小田英之、西川明花、升田博隆、岡野栄之、松崎有未、青木大輔、吉村泰典
  • Organizer: 第61回日本産科婦人科学会学術講演会
  • Place of Presentation: 京都
  • Year and Date: 20090403-20090405
• [Presentation] The Role of uterine smooth muscle stem cells, isolated based on cell surface markers, in myometrial remodeling in the pregnant uterus. (2009)
  • Author(s): Ono M, Maruyama T, Kajitani T, Uchida H, Oda H, Nishikawa S, Kagami M, Arase T, Nagashima T, Masuda H, Morikawa S, Mabuchi Y, Okano H, Matsuzaki Y, Yoshimura Y
  • Organizer: Society for Gynecologic Investigation's 55th Annual Scientific Meeting
  • Place of Presentation: Glasgow, Scotland
  • Year and Date: 20090317-20090321
• [Presentation] 子宮内膜症組織におけるリポキシゲナーゼ経路関連遺伝子の発現解析. (2009)
  • Author(s): 梶谷宇, 丸山哲夫, 浅田弘法, 内田浩, 各務真紀, 小田英之, 西川明花, 山崎彰子, 吉村泰典
  • Organizer: 第30回日本エンドメトリオーシス学会
  • Place of Presentation: 仙台
  • Year and Date: 20090117-20090118
• [Presentation] CE-TOFMSによる子宮内膜症患者腹水のメタボローム解析. (2009)
  • Author(s): 川田陽子, 浅田弘法, 杉本昌弘, 平山明由, 阿部しのぶ, 古谷正敬, 内田浩, 浜谷敏生, 梶谷宇, 丸山哲夫, 吉村泰典, 曽我朋義, 冨田勝
  • Organizer: 第30回日本エンドメトリオーシス学会
  • Place of Presentation: 仙台
  • Year and Date: 20090117-20090118
• [Presentation] 腹腔鏡下ダグラス窩子宮内膜症病巣切除術の定型化と疼痛緩和効果に関する検討. (2009)
  • Author(s): 浅田弘法, 古谷正敬, 梶谷宇, 内田浩, 丸山哲夫, 吉村泰典, 升田博隆, 岸郁子, 田島敏秀, 寺西貴英, 木挽貢慈
  • Organizer: 第30回日本エンドメトリオーシス学会
  • Place of Presentation: 仙台
  • Year and Date: 20090117-20090118
• [Presentation] 子宮内膜におけるP2RY14を介した新たな粘膜防御機構. (2008)
  • Author(s): 荒瀬透, 丸山哲夫, 内田浩, 梶谷宇, 西川明花, 小田英之, 各務真紀, 浅田弘法, 吉村泰典
  • Organizer: 第23回日本生殖免疫学会学術集会
  • Place of Presentation: 富山
  • Year and Date: 20081206-20081207
• [Presentation] Laparoscopic treatment of rectovaginal endometriosis(Japanese experience). (2008)
  • Author(s): Asada H, Furuya M, Uchida H, Maruyama T, Kobiki K, Kajitani T, Yoshimura Y
  • Organizer: 9th Annual Congress of APAGE
  • Place of Presentation: Daegu, Korea
  • Year and Date: 20081009-20081012
• [Presentation] Isolation and functional analysis of putative human endometrial stem/progenitor cells. (2008)
  • Author(s): Masuda H, Maruyama T, Ono M, Kajitani T, Okano H, Yoshimura Y
  • Organizer: The 24th European Society of Human Reproduction and Embryology.
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20080706-20080709
• [Presentation] 子宮平滑筋幹細胞の新しい分離法とその機能解析. (2008)
  • Author(s): 小野政徳, 丸山哲夫, 梶谷宇, 内田浩, 荒瀬透, 小田英之, 西川明花, 長島隆, 升田博隆, 馬渕洋, 森川暁, 岡野栄之, 松崎有未, 吉村泰典
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: 青森
  • Year and Date: 20080516-20080518
• [Presentation] ヒト着床モデルにおけるepithelial-to-mesenchymal transition -N-cadherinの時期特異的機能関与-. (2008)
  • Author(s): 内田浩, 丸山哲夫, 荒瀬透, 小野政徳, 各務真紀, 小田英之, 西川明花, 梶谷宇, 浅田弘法, 青木大輔, 吉村泰典
  • Organizer: 第60回日本産婦人科学会学術講演
  • Place of Presentation: 横浜
  • Year and Date: 20080412-20080415
• [Presentation] ヒト雌性生殖器官における新しい感染防御システム-g蛋白共役型受容体P2Y14とそのリガンドUDP-glucose-. (2008)
  • Author(s): 荒瀬透, 丸山哲夫, 内田浩, 梶谷宇, 小野政徳, 小田英之, 西川明花, 各務真紀, 浅田弘法, 青木大輔, 吉村泰典
  • Organizer: 第60回日本産婦人科学会学術講演会
  • Place of Presentation: 横浜
  • Year and Date: 20080412-20080415
• [Presentation] ヒストン脱アセチル化酵素阻害剤HC toxinによるhCG分泌および細胞増殖を標的にした絨毛性疾患の制御. (2008)
  • Author(s): 太田邦明, 丸山哲夫, 内田浩, 小野政徳, 梶谷宇, 荒瀬透, 森田峰人, 吉村泰典
  • Organizer: 第60回日本産婦人科学会学術講演会
  • Place of Presentation: 横浜
  • Year and Date: 20080412-20080415
• [Presentation] UDP-glucose and its receptor P2Y14 as a new innate immune system in the female reproductive tract. (2008)
  • Author(s): Arase T, Maruyama T, Uchida H, Kajitani T, Ono M, Kagami M, Asada H, Yoshimura Y
  • Organizer: Society for Gynecologic Investigation's 55th Annual Scientific Meeting.
  • Place of Presentation: San Diego, USA
  • Year and Date: 20080326-20080329
• [Presentation] N-Cadherin Regulates Epithelio-Mesenchymal Transition and Endometrial Ephithelial Cell Dynamics during Human Implantation. (2008)
  • Author(s): Uchida H, Maruyama T, Arase T, Ono M, Kajitani T, Kagami M, Oda H, Nishikawa S, Yoshimura Y
  • Organizer: Society for Gynecologic Investigation's 55th Annual Scientific Meeting.
  • Place of Presentation: San Diego, USA
  • Year and Date: 20080326-20080329
• [Presentation] Expression analysis of pain-related genesin endometriosis and adenomyosis. (2008)
  • Author(s): Kajitani T, Maruyama T, Asada H, Ono M, Arase T, Kagami M, Oda H, Nishikawa S, Uchida H, Yoshimura Y
  • Organizer: 10th World Congress on Endometriosis.
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20080311-20080314
• [Presentation] Genome-wide approaches for identification of novel endometriosis-associated genes. (2008)
  • Author(s): Maruyama T, Kajitani T, Oda H, Nishikawa S, Arase T, Kagami M, Ono M, Asada H, Uchida H, Yoshimura Y
  • Organizer: 10th World Congress on Endometriosis.
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20080311-20080314
• [Presentation] 子宮内膜症・腺筋症由来疼痛に関連する遺伝子の発現解析. (2008)
  • Author(s): 梶谷宇, 丸山哲夫, 浅田弘法, 小野政徳, 荒瀬透, 小田英之, 西川明花, 内田浩, 吉村泰典
  • Organizer: 第29回エンドメトリオーシス研究会
  • Place of Presentation: 高知
  • Year and Date: 20080119-20080120
• [Presentation] 子宮内膜症関連遺伝子同定のための新しいアプローチ. (2008)
  • Author(s): 丸山哲夫, 梶谷宇, 小田英之, 西川明花, 荒瀬透, 内田浩, 浅田弘法, 吉村泰典
  • Organizer: 第29回エンドメトリオーシス研究会
  • Place of Presentation: 高知
  • Year and Date: 20080119-20080120
• [Presentation] ヒト子宮内膜幹様細胞の同定・分離とその幹細胞特性の機能解析. (2008)
  • Author(s): 升田博隆, 丸山哲夫, 小野政徳, 長島隆, 梶谷宇, 内田浩, 浅田弘法, 岡野栄之, 松崎有未, 吉村泰典
  • Organizer: 第13回日本生殖内分泌学会
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-29
• [Presentation] 核移行転写因子STAT5の上流シグナル分子としてSRCチロシンキナーゼはヒト子宮内膜脱落膜化を制御する. (2007)
  • Author(s): 長島隆, 丸山哲夫, 内田浩, 梶谷宇, 小野政徳, 荒瀬透, 各務真紀, 浅田弘法, 吉村泰典
  • Organizer: 第52回日本生殖医学会学術講演会
  • Place of Presentation: 秋田
  • Year and Date: 20071025-20071026
• [Presentation] OCT-4 expression in human myometrium. (2007)
  • Author(s): Ono M, Maruyama T, Kajitani T, Uchida H, Masuda H, Nagashima T, Arase T, Ohta K, Asada H, Yoshimura Y
  • Organizer: AOCOG2007
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20070921-20070925
• [Presentation] ヒト子宮平滑筋成体幹細胞の同定とその機能解析. (2007)
  • Author(s): 小野政徳, 丸山哲夫, 升田博隆, 梶谷宇, 長島隆, 荒瀬透, 太田邦明, 各務真紀, 小田英之, 浅田弘法, 伊藤守, 内田浩, 岡野栄之, 松崎有未, 吉村泰典
  • Organizer: 第49回日本平滑筋学会総会
  • Place of Presentation: 奈良
  • Year and Date: 20070705-20070706
• [Presentation] Src kinase activity is essential for decidualization of human endometrial stromal cells. (2007)
  • Author(s): Nagashima T, Maruyama T, Uchida H, Kajitani T, Ono M, Arase T, Kagami M, Ohta K, Asada H, Yoshimura Y
  • Organizer: The 23th Annual Meeting of the European Society of Human Reproduction and Embryology
  • Place of Presentation: Lyon, France
  • Year and Date: 20070701-20070704
• [Presentation] Reconstruction and in vivo bioluminescence imaging of human endometrium in immunodeficient mice: its potential application as an endometriosis model. (2007)
  • Author(s): Masuda H, Maruyama T, Nagashima T, Ono M, Kajitani T, Ohta K, Arase T, Kagami M, Asada H, Okano H, Matsuzaki Y, Yoshimura Y
  • Organizer: The 23th Annual Meeting of the European Society of Human Reproduction and Embryology
  • Place of Presentation: Lyon, France
  • Year and Date: 20070701-20070704
• [Presentation] 疼痛関連遺伝子Nerve Growth Factorの子宮内膜症・腺筋症における発現解析. (2007)
  • Author(s): 梶谷宇、丸山哲夫、浅田弘法、内田浩、升田博隆、長島隆、小野政徳、荒瀬透、各務真紀、太田邦明、吉村泰典
  • Organizer: 第80回日本内分泌学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 20070614-20070616
• [Presentation] Srcキナーゼ活性はヒト子宮内膜間質細胞の脱落膜化に必須である. (2007)
  • Author(s): 長島隆, 丸山哲夫, 内田浩, 梶谷宇, 荒瀬透, 小野政徳, 各務真紀, 吉村泰典
  • Organizer: 第80回日本内分泌学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 20070614-20070616
• [Presentation] ヒト子宮内膜再生モデルマウスを用いた内膜幹細胞解析システムの開発. (2007)
  • Author(s): 升田博隆, 丸山哲夫, 長島隆, 小野政徳, 梶谷宇, 内田浩, 浅田弘法, 岡野栄之, 松崎有未, 青木大輔, 吉村泰典
  • Organizer: 第59回日本産科婦人科学会学術講演会
  • Place of Presentation: 京都
  • Year and Date: 20070414-20070417
• [Presentation] アデノウィルスベクターによるSrcチロシンキナーゼを標的にしたヒト子宮内膜脱落膜化の制御. (2007)
  • Author(s): 長島隆, 丸山哲夫, 内田浩, 梶谷宇, 荒瀬透, 小野政徳, 各務真紀, 太田邦明, 青木大輔, 吉村泰典
  • Organizer: 第59回日本産科婦人科学会学術講演会
  • Place of Presentation: 京都
  • Year and Date: 20070414-20070417
• [Presentation] 子宮内膜感染防御におけるG蛋白共役型受容体P2Y14/GPR105の役割. (2007)
  • Author(s): 荒瀬透, 丸山哲夫, 内田浩, 梶谷宇, 小野政徳, 長島隆, 各務真紀, 太田邦明, 青木大輔, 吉村泰典
  • Organizer: 第59回日本産科婦人科学会学術講演会
  • Place of Presentation: 京都
  • Year and Date: 20070414-20070417
• [Remarks]
  • URL: http://www.keio.ac.jp/english/research/atoz_lifesci.html