Cbln1, novel synaptogenic factor secreted from presynaptic site〜The mechanism of its acceptance.

2008 Fiscal Year Final Research Report

• Project/Area Number: 19700349
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Keio University
• Principal Investigator: MATSUDA Keiko Keio University, 医学部, 助教 (40383765)
• Project Period (FY): 2007 – 2008
• Keywords: 細胞内情報伝達 / シナプス / 小脳

Research Abstract

Cbln1分子は小脳顆粒細胞軸索-プルキンエ細胞間でのシナプスの形成・維持と機能的可塑性に必須である分泌性因子である。我々はCbln1全長がリガンドとして働き、その受容体がプルキンエ細胞神経棘上に特異的に局在することを示した。申請者はCbln1と共役して働く受容体候補の膜タンパク質を同定し、この受容体がCbln1によるシナプス形成において必須な受容体複合体の一部であることを見出した。

Research Products

• [Journal Article] The N-terminal domain of GluD2 (GluRδ2) recruits presynaptic terminals and regulates synaptogenesis in the cerebellum in vivo. (2009)
  • Author(s): Kakegawa, W., Miyazaki, T., Kohda, K., Matsuda, K., Emi, K., Motohashi, J., Watanabe, M., Yuzaki, M.
  • Journal Title: Journal of Neuroscience 29 Pages: 5738-5748
  • Peer Reviewed
• [Journal Article] Cbln1 binds to specific postsynaptic sites at parallel fiber-Purkinje cell synapses in the cerebellum. (2009)
  • Author(s): Matsuda K., Kondo, T., Iijima, T., Matsuda, S., Watanabe, M., Yuzaki, M.
  • Journal Title: European Journal of Neuroscience 29 Pages: 707-717
  • Peer Reviewed
• [Journal Article] Cbln1 accumulates and colocalizes with Cbln3 and GluRδ2 at parallel fiber-Purkinje cell synapses in the mouse cerebellum. (2009)
  • Author(s): Miura, E., Matsuda K., Morgan, J.I., Yuzaki, M., Watanabe, M.
  • Journal Title: European Journal of Neuroscience 29 Pages: 693-706
  • Peer Reviewed
• [Journal Article] Cbln1 Regulates Rapid Formation and Maintenance of Excitatory Synapses in Mature Cerebellar Purkinje Cells in vitro and in vivo. (2008)
  • Author(s): Ito-Ishida, A., Miura, E., Emi, K., Matsuda K., Iijima, T., Kondo, T., Kohda, K. Watanabe, M., Yuzaki, M.
  • Journal Title: Journal of Neuroscience 28 Pages: 5920-5930
  • Peer Reviewed
• [Journal Article] Accumulation of AMPA Receptors in Autophagosomes in Neuronal Axons Lacking Adaptor Protein AP-4. (2008)
  • Author(s): Matsuda S., Miura E., Matsuda K., Kakegawa W., Kohda K., Watanabe M., Yuzaki M.
  • Journal Title: Neuron 57 Pages: 730-745
  • Peer Reviewed
• [Journal Article] Differential Regulation of Synaptic Plasticity and Cerebellar Motor Learning by the C-terminal PDZ-Binding Motif of GluRdelta2. (2008)
  • Author(s): Kakegawa W., Miyazaki T., Emi K., Matsuda K., Kohda K., Motohash, J., Mishina M., Kawahara S., Watanabe M., Yuzaki M.
  • Journal Title: Journal of Neuroscience 28 Pages: 1460-1468
  • Peer Reviewed
• [Journal Article] Characterization of a transneuronal cytokine family Cbln-Regulation of secretion by heteromeric assembly. (2007)
  • Author(s): Iijima I, Miura E, Matsuda K ., Kamekawa Y, Watanabe M, Yuzaki M
  • Journal Title: European Journal of Neuroscience 25 Pages: 1049-1057
  • Peer Reviewed
• [Presentation] 神経細胞におけるイオンチャンネル足場タンパク質から核への信号伝達経路の解析 (2007)
  • Author(s): 松田恵子 柚崎通介
  • Organizer: 第30回日本分子生物学会第80回日本生化学会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 20071211-14
• [Presentation] Mechanism of hexametric Cbln1 ligand binding to PSD fraction prepared from cerebellum : implication of Cbln1 receptor protein in Purkinje cells. (2007)
  • Author(s): Matsuda, K., Kondo, T., Iijima, T., Ishida,A., Yuzaki, M.
  • Organizer: The 30th Annual Meeting of the Japan Neuroscience Society.
  • Place of Presentation: 横浜
  • Year and Date: 20070910-12