2009 Fiscal Year Final Research Report
Site-selective anatomy of molecular interactions using dynamic force spectroscopy
| Project/Area Number |
19710109
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | University of Tsukuba |
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Principal Investigator |
TANINAKA Atsushi University of Tsukuba, 大学院・数理物質科学研究科, 助教 (80400638)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 1分子ナノ計測 |
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| Research Abstract |
We have demonstrated a methodology that realizes the site-selective anatomy of molecular interactions at the single-molecule level. With the combination of cross-linkers and the atomic force microscope that we developed to enable a precise analysis by dynamic force spectroscopy, direct and bridged interactions at each reaction site in a typical ligand-receptor system, sreptavidin-biotin complex, were clearly distinguished and individually analyzed for the first time, providing a greater understanding of step-by-step progress of the bonding process.
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