Molecular sorting system using kinesin and dynein motor protein

2008 Fiscal Year Final Research Report

• Project/Area Number: 19710111
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Ritsumeikan University
• Principal Investigator: YOKOKAWA Ryuji Ritsumeikan University, 理工学部, 講師 (10411216)
• Project Period (FY): 2007 – 2008
• Keywords: ナノソーティング / キネシン / ダイニン / MicroTAS / MEMS

Research Abstract

モータタンパク質であるキネシンとダイニンの微小管上での運動極性を利用して、微小流体デバイス内でナノスケールの対象分子などを大量・並列に操作し、分離することのできるナノアクチュエータの創出を目指した。微小管の極性配置技術を確立し、二種類のモータタンパク質が極性に従って効率よく運動する系を微小流体デバイス内で構築した。

Research Products

• [Journal Article] Versatile Microfluidic Total Internal Reflection (TIR)-based Devices: Application to Microbeads Velocity Measurement and Single Molecule Detection with Upright and Inverted Microscope (2009)
  • Author(s): N. C. H. Le, R. Yokokawa, D. V. Dao, T. D. Nguyen, J. Wells, and S. Sugiyama,
  • Journal Title: Lab Chip vol. 9 Pages: 244-250
  • Peer Reviewed
• [Journal Article] DNA molecule manipulation by motor proteins for its evaluation in single molecule level (2008)
  • Author(s): R. Yokokawa, J. Miwa, M. C. Tarhan, H. Fujita, and M. Kasahara
  • Journal Title: Anal. Bioanal. Chem vol. 391 Pages: 2735-43
  • Peer Reviewed
• [Journal Article] Simultaneous and Bidirectional Transport of Kinesin-Coated Microspheres and Dynein-Coated Microspheres on Polarity-Oriented Microtubules (2008)
  • Author(s): R. Yokokawa, M. C. Tarhan, T. Kon, and H. Fujita
  • Journal Title: Biotechnol. Bioeng vol. 101 Pages: 1-8
  • Peer Reviewed
• [Presentation] Development and Application of Tir-Based Chip Based on Mems Thechnology (2008)
  • Author(s): R. Yokokawa, N. C. H. Le, D. V. Dao, J. C. Wells, and S. Sugiyama
  • Organizer: The first International Symposium on Micro/Nano Systems Technology
  • Place of Presentation: Hanoi, VietNam
  • Year and Date: 20081218-21
• [Presentation] Kinesin-based transportation of hydrophobic and hydrophilic micro-containers (2008)
  • Author(s): C. Bottier, M.C. Tarhan, J. Fattaccioli, R. Yokokawa, D. Collard, H. Fujita
  • Organizer: International Symposium on Microchemistry and Microsystems (ISMM2008)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20081207-08
• [Presentation] Kinesin-Based Transportation and Electrofusion of Lipid Vesicles (2008)
  • Author(s): C. Bottier, M. C. Tarhan, D. Collard, R. Yokokawa, and H. Fujita
  • Organizer: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (・TAS2008)
  • Place of Presentation: San Diego, USA
  • Year and Date: 20081012-16
• [Presentation] Isolation and Manipulation of Single Microtubule by Silicon Microtweezers (2008)
  • Author(s): M. C. Tarhan, D. Collard, C. Bottier, R. Yokokawa, M. Hosogi, G. Hashiguchi, and H. Fujita,
  • Organizer: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: San Diego, USA
  • Year and Date: 20081012-16
• [Presentation] Effective Dilution of Protein for Single Molecule Assay in an Integrated Assay Device (2008)
  • Author(s): T. Nakayama, K. Tabata, H. Noji, and R. Yokokawa
  • Organizer: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (・TAS2008)
  • Place of Presentation: San Diego, USA
  • Year and Date: 20081012-16
• [Patent(Industrial Property Rights)] 蛍光顕微鏡用全反射バイオチップ及びその製法、並びに蛍光顕微鏡用全反射バイオチップアセンブリ (2008)
  • Inventor(s): 杉山進、横川隆司、ジョンシーウェルズ、ナムカオホアイレ、ズンベトダオ
  • Industrial Property Rights Holder: 学校法人立命館
  • Industrial Property Number: 特願2008-119835
  • Filing Date: 2008-05-01
• [Patent(Industrial Property Rights)] 蛍光顕微鏡用全反射バイオチップ及びその製法、並びに蛍光顕微鏡用全反射バイオチップアセンブリ (2007)
  • Inventor(s): 杉山進、横川隆司、ジョンシーウェルズ、ナムカオホアイレ、ズンベトダオ
  • Industrial Property Rights Holder: 学校法人立命館
  • Industrial Property Number: 特願2007-121901
  • Filing Date: 2007-05-02